Calcium in PDB 4gx5: Gsuk Channel

The structure of Gsuk Channel, PDB code: 4gx5 was solved by C.Kong, W.Zeng, S.Ye, L.Chen, D.B.Sauer, Y.Lam, M.G.Derebe, Y.Jiang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.39 / 3.70
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	234.976, 108.364, 165.787, 90.00, 134.96, 90.00
R / Rfree (%)	26.1 / 29.3

The structure of Gsuk Channel also contains other interesting chemical elements:

Potassium	(K)	12 atoms
Zinc	(Zn)	4 atoms

The binding sites of Calcium atom in the Gsuk Channel (pdb code 4gx5). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Gsuk Channel, PDB code: 4gx5:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Gsuk Channel


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Gsuk Channel within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca606 b:0.4 occ:1.00 O A:GLU449 2.9 0.8 1.0 O B:THR183 3.0 0.0 1.0 OD1 A:ASN450 3.1 0.4 1.0 OG1 B:THR214 3.3 0.8 1.0 OE1 A:GLN453 3.4 0.4 1.0 OD1 B:ASN210 3.4 0.6 1.0 CG A:ASN450 3.6 0.9 1.0 C A:GLU449 3.6 0.9 1.0 CA A:ASN450 3.6 0.8 1.0 C B:THR183 3.8 0.8 1.0 N A:ASN450 3.9 0.4 1.0 CD A:GLN453 3.9 0.2 1.0 CG2 B:THR214 4.0 0.8 1.0 CG A:GLN453 4.1 0.2 1.0 CB A:ASN450 4.2 0.7 1.0 ND2 A:ASN450 4.2 0.6 1.0 CB B:THR214 4.2 0.4 1.0 CB B:THR183 4.3 0.0 1.0 CG A:GLU449 4.3 0.8 1.0 CG B:ASN210 4.4 0.2 1.0 CA B:THR183 4.5 0.5 1.0 ND2 B:ASN210 4.6 0.3 1.0 OG1 B:THR183 4.6 0.2 1.0 CB A:GLU449 4.6 0.5 1.0 N B:ASP184 4.6 0.7 1.0 CB A:GLN453 4.6 0.3 1.0 CA A:GLU449 4.7 0.7 1.0 C A:ASN450 4.8 0.7 1.0 N B:ALA185 4.8 0.0 1.0 NE2 A:GLN453 4.8 0.6 1.0 CA B:ASP184 4.8 0.2 1.0

Calcium binding site 2 out of 4 in the Gsuk Channel


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Gsuk Channel within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca602 b:0.8 occ:1.00 OD1 B:ASN450 3.0 0.5 1.0 OE1 B:GLN453 3.1 0.9 1.0 O B:GLU449 3.1 0.2 1.0 CD B:GLN453 3.5 0.4 1.0 CA B:ASN450 3.6 1.0 1.0 CG B:ASN450 3.6 1.0 1.0 CG B:GLN453 3.6 0.0 1.0 C B:GLU449 3.8 0.4 1.0 N B:ASN450 4.1 0.5 1.0 CB B:GLN453 4.1 0.9 1.0 CB B:ASN450 4.1 0.8 1.0 ND2 B:ASN450 4.4 0.3 1.0 NE2 B:GLN453 4.5 0.2 1.0 C B:ASN450 4.6 0.9 1.0 CG B:GLU449 4.8 0.6 1.0 O B:ASN450 4.9 0.2 1.0

Calcium binding site 3 out of 4 in the Gsuk Channel


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Gsuk Channel within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca606 b:0.2 occ:1.00 O C:THR183 3.0 0.6 1.0 OG1 C:THR214 3.0 0.6 1.0 OD1 C:ASN210 3.4 0.4 1.0 CG2 C:THR214 3.8 0.3 1.0 C C:THR183 3.9 0.9 1.0 CB C:THR214 4.0 0.1 1.0 CG C:ASN210 4.4 0.2 1.0 CB C:THR183 4.4 1.0 1.0 CA C:THR183 4.6 0.5 1.0 ND2 C:ASN210 4.7 0.3 1.0 OG1 C:THR183 4.8 0.7 1.0 N C:ASP184 4.8 1.0 1.0 N C:ALA185 4.8 0.8 1.0 O C:ASN210 5.0 0.4 1.0 CA C:ASP184 5.0 0.2 1.0

Calcium binding site 4 out of 4 in the Gsuk Channel


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Gsuk Channel within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca607 b:0.1 occ:1.00 OD1 C:ASN450 2.9 0.1 1.0 O C:GLU449 3.0 0.4 1.0 O D:THR183 3.0 0.6 1.0 OD1 D:ASN210 3.3 0.9 1.0 OE1 C:GLN453 3.4 0.1 1.0 CG C:ASN450 3.4 0.6 1.0 CA C:ASN450 3.5 0.8 1.0 C C:GLU449 3.6 0.6 1.0 OG1 D:THR214 3.6 0.6 1.0 C D:THR183 3.7 0.7 1.0 N C:ASN450 3.8 0.2 1.0 CD C:GLN453 3.9 0.2 1.0 CB D:THR183 4.0 0.3 1.0 CB C:ASN450 4.0 0.9 1.0 ND2 C:ASN450 4.1 0.1 1.0 CG C:GLN453 4.1 0.8 1.0 OG1 D:THR183 4.1 0.2 1.0 CG D:ASN210 4.2 0.7 1.0 ND2 D:ASN210 4.2 0.1 1.0 CG C:GLU449 4.3 0.1 1.0 CA D:THR183 4.3 0.8 1.0 N D:ASP184 4.4 0.3 1.0 CG2 D:THR214 4.5 0.6 1.0 CB C:GLN453 4.5 0.3 1.0 C C:ASN450 4.7 0.8 1.0 CB D:THR214 4.7 0.0 1.0 CB C:GLU449 4.7 0.2 1.0 CA D:ASP184 4.7 0.0 1.0 CA C:GLU449 4.8 0.1 1.0 NE2 C:GLN453 4.9 0.5 1.0 N D:ALA185 4.9 0.8 1.0

C.Kong, W.Zeng, S.Ye, L.Chen, D.B.Sauer, Y.Lam, M.G.Derebe, Y.Jiang. Distinct Gating Mechanisms Revealed By the Structures of A Multi-Ligand Gated K(+) Channel. Elife V. 1 00184 2012.
PubMed: 23240087
DOI: 10.7554/ELIFE.00184