Calcium in PDB 4hht: T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions

All present enzymatic activity of T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions:
3.1.26.4;

The structure of T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions, PDB code: 4hht was solved by M.P.Rychlik, M.Nowotny, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.02 / 3.10
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	106.952, 47.626, 77.451, 90.00, 131.26, 90.00
R / Rfree (%)	17.8 / 25.4

The binding sites of Calcium atom in the T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions (pdb code 4hht). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions, PDB code: 4hht:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:43.6 occ:1.00 O D:HOH104 2.6 31.6 1.0 OP2 D:C6 2.6 32.4 1.0 OD2 A:ASP124 2.9 31.9 1.0 O D:HOH103 3.1 24.2 1.0 O D:HOH101 3.2 19.9 1.0 OD1 A:ASP124 3.3 38.7 1.0 CG A:ASP124 3.4 38.4 1.0 NE2 A:GLN125 3.6 52.1 1.0 P D:C6 3.9 34.3 1.0 OP1 D:C6 4.3 33.2 1.0 OD2 A:ASP107 4.6 37.3 1.0 CD A:GLN125 4.8 53.1 1.0 O3' D:DC5 4.8 37.3 1.0 CB A:ASP124 4.9 33.1 1.0 CG A:LYS122 4.9 39.6 1.0 O5' D:C6 4.9 29.0 1.0 OP1 D:DC5 5.0 43.1 1.0

Calcium binding site 2 out of 2 in the T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of T. Maritima Rnase H2 G21S in Complex with Nucleic Acid Substrate and Calcium Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca302 b:28.2 occ:1.00 OP1 D:C6 2.4 33.2 1.0 OD2 A:ASP18 2.5 30.0 1.0 OP2 D:DT7 2.5 34.9 1.0 O A:GLU19 2.7 28.7 1.0 O A:HOH410 2.7 26.6 1.0 O D:HOH102 3.0 27.8 1.0 CG A:ASP18 3.2 32.7 1.0 OD1 A:ASP18 3.3 30.6 1.0 P D:C6 3.6 34.3 1.0 O5' D:C6 3.7 29.0 1.0 C A:GLU19 3.9 29.4 1.0 P D:DT7 3.9 32.8 1.0 C5' D:C6 4.0 32.1 1.0 OD2 A:ASP124 4.1 31.9 1.0 CB A:SER134 4.1 26.6 1.0 N A:GLU19 4.4 33.7 1.0 OP2 D:C6 4.4 32.4 1.0 OD2 A:ASP107 4.4 37.3 1.0 O3' D:C6 4.4 30.3 1.0 CB A:ASP18 4.5 27.0 1.0 CA A:SER134 4.6 27.4 1.0 CA A:ALA20 4.7 34.1 1.0 O5' D:DT7 4.7 25.9 1.0 OE2 A:GLU19 4.8 42.0 1.0 O3' D:DC5 4.8 37.3 1.0 N A:ALA20 4.8 31.7 1.0 CA A:GLU19 4.8 30.6 1.0 OP1 D:DT7 4.9 32.7 1.0 O A:SER134 4.9 28.0 1.0 C3' D:C6 4.9 35.8 1.0 CD A:GLU19 5.0 42.8 1.0 C4' D:C6 5.0 34.2 1.0 CG A:ASP124 5.0 38.4 1.0

H.Chon, J.L.Sparks, M.Rychlik, M.Nowotny, P.M.Burgers, R.J.Crouch, S.M.Cerritelli. Rnase H2 Roles in Genome Integrity Revealed By Unlinking Its Activities. Nucleic Acids Res. V. 41 3130 2013.
ISSN: ISSN 0305-1048
PubMed: 23355612
DOI: 10.1093/NAR/GKT027