Calcium in PDB 4i5k: PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz

The structure of PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz, PDB code: 4i5k was solved by Y.Xing, P.D.Jeffrey, Y.Shi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.93 / 2.90
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	132.812, 109.086, 67.651, 90.00, 109.05, 90.00
R / Rfree (%)	20.7 / 25.8

The binding sites of Calcium atom in the PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz (pdb code 4i5k). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz, PDB code: 4i5k:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca501 b:61.6 occ:1.00 OD1 A:ASP294 2.1 56.8 1.0 OD1 A:ASP290 2.2 48.7 1.0 O A:TYR296 2.3 50.0 1.0 OD2 A:ASP292 2.3 95.7 1.0 OD2 A:ASP301 2.3 58.6 1.0 CG A:ASP292 3.0 75.2 1.0 CG A:ASP294 3.2 54.2 1.0 OD1 A:ASP292 3.3 87.8 1.0 CG A:ASP301 3.3 60.8 1.0 CG A:ASP290 3.4 48.0 1.0 C A:TYR296 3.5 53.2 1.0 OD2 A:ASP294 3.6 72.8 1.0 OD1 A:ASP301 3.6 66.5 1.0 N A:ASP294 4.1 39.0 1.0 CA A:ASP290 4.1 45.5 1.0 N A:TYR296 4.1 53.2 1.0 N A:ASP292 4.2 57.1 1.0 OD2 A:ASP290 4.3 44.1 1.0 CB A:ASP292 4.3 47.6 1.0 CB A:ASP290 4.3 48.0 1.0 N A:HIS293 4.4 43.1 1.0 C A:ASP290 4.4 45.1 1.0 CA A:TYR296 4.4 55.5 1.0 N A:ILE297 4.4 55.0 1.0 CB A:ASP294 4.4 43.2 1.0 CA A:ILE297 4.4 48.6 1.0 N A:SER298 4.5 51.0 1.0 CA A:ASP292 4.5 46.2 1.0 C A:ASP292 4.5 53.9 1.0 N A:THR291 4.6 48.1 1.0 CB A:ASP301 4.7 67.0 1.0 CA A:ASP294 4.7 39.6 1.0 N A:LEU295 4.7 45.0 1.0 OG A:SER298 4.8 51.3 1.0 O A:ASP290 4.9 55.8 1.0 C A:ILE297 4.9 46.6 1.0 C A:ASP294 4.9 48.4 1.0 CB A:TYR296 4.9 53.2 1.0

Calcium binding site 2 out of 4 in the PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:63.0 occ:1.00 O A:VAL370 2.0 32.8 1.0 OD1 A:ASP368 2.3 93.6 1.0 OE1 A:GLU375 2.4 55.5 1.0 OD1 A:ASP364 2.4 58.1 1.0 OE2 A:GLU375 2.4 35.0 1.0 OD1 A:ASP366 2.4 67.9 1.0 CD A:GLU375 2.7 60.8 1.0 CG A:ASP368 2.9 89.5 1.0 OD2 A:ASP368 3.0 0.2 1.0 CG A:ASP366 3.1 66.4 1.0 OD2 A:ASP366 3.2 74.2 1.0 C A:VAL370 3.3 56.6 1.0 CG A:ASP364 3.5 61.3 1.0 N A:SER372 4.0 74.3 1.0 CA A:ASP364 4.1 54.8 1.0 CA A:LEU371 4.1 50.4 1.0 N A:LEU371 4.1 45.0 1.0 CG A:GLU375 4.2 47.4 1.0 N A:VAL370 4.2 61.5 1.0 CB A:ASP364 4.3 56.2 1.0 OG A:SER372 4.3 67.1 1.0 CA A:VAL370 4.3 63.1 1.0 CB A:ASP368 4.3 64.3 1.0 N A:ASP368 4.3 62.8 1.0 OD2 A:ASP364 4.4 71.2 1.0 CG2 A:VAL370 4.4 82.4 1.0 C A:ASP364 4.4 60.4 1.0 N A:ASP366 4.5 67.7 1.0 N A:VAL365 4.5 54.6 1.0 CB A:ASP366 4.5 53.6 1.0 C A:LEU371 4.6 63.6 1.0 N A:GLY367 4.6 60.2 1.0 CB A:SER372 4.7 62.4 1.0 CA A:ASP368 4.8 61.8 1.0 CD2 A:LEU371 4.9 74.1 1.0 N A:GLY369 4.9 65.8 1.0 CA A:ASP366 4.9 62.2 1.0 CA A:SER372 5.0 58.8 1.0 CB A:GLU375 5.0 45.4 1.0

Calcium binding site 3 out of 4 in the PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca501 b:98.0 occ:0.50 OD1 B:ASP294 2.4 94.6 1.0 O B:TYR296 2.4 99.2 1.0 OD1 B:ASP290 2.5 0.2 1.0 OD2 B:ASP292 2.5 0.1 1.0 OD2 B:ASP294 2.6 84.4 1.0 OD2 B:ASP301 2.7 0.2 1.0 CG B:ASP294 2.8 96.5 1.0 CG B:ASP292 3.6 0.2 1.0 CG B:ASP290 3.6 99.5 1.0 C B:TYR296 3.6 96.3 1.0 CA B:ASP290 3.7 0.6 1.0 CG B:ASP301 3.9 0.3 1.0 OD1 B:ASP292 4.0 0.1 1.0 CB B:ASP290 4.2 0.3 1.0 CA B:ILE297 4.2 93.0 1.0 C B:ASP290 4.3 0.5 1.0 CB B:ASP294 4.3 0.4 1.0 N B:THR291 4.4 0.2 1.0 N B:ILE297 4.4 95.6 1.0 O B:LEU289 4.5 86.5 1.0 N B:SER298 4.5 89.3 1.0 N B:ASP292 4.5 0.1 1.0 NH2 B:ARG334 4.6 0.7 1.0 OD1 B:ASP301 4.6 0.4 1.0 OD2 B:ASP290 4.7 74.7 1.0 N B:ASP290 4.7 0.5 1.0 CD1 B:ILE297 4.7 0.5 1.0 N B:ASP294 4.7 0.8 1.0 CA B:TYR296 4.8 95.1 1.0 N B:TYR296 4.8 97.0 1.0 C B:ILE297 4.8 84.8 1.0 CB B:ASP301 4.8 1.0 1.0 CB B:ASP292 4.9 94.0 1.0 C B:LEU289 5.0 80.8 1.0

Calcium binding site 4 out of 4 in the PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of PP2A PR70 Holoenzyme MODEL3_DICA_RCSB.Pdb BPPNAT5_EXTEND.Mtz within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca502 b:60.5 occ:1.00 O B:VAL370 2.3 41.8 1.0 OD1 B:ASP366 2.3 73.6 1.0 OD1 B:ASP364 2.3 73.1 1.0 OD1 B:ASP368 2.4 0.7 1.0 OE1 B:GLU375 2.4 52.1 1.0 OE2 B:GLU375 2.5 44.2 1.0 CD B:GLU375 2.7 43.8 1.0 CG B:ASP368 3.0 88.9 1.0 OD2 B:ASP368 3.0 98.3 1.0 CG B:ASP366 3.0 62.2 1.0 OD2 B:ASP366 3.1 60.9 1.0 CG B:ASP364 3.5 69.8 1.0 C B:VAL370 3.5 48.9 1.0 N B:SER372 4.0 52.4 1.0 CG B:GLU375 4.2 36.2 1.0 OG B:SER372 4.2 48.8 1.0 CA B:ASP364 4.2 50.1 1.0 OD2 B:ASP364 4.3 64.6 1.0 CA B:LEU371 4.3 48.5 1.0 N B:ASP368 4.3 56.7 1.0 CB B:ASP368 4.4 73.5 1.0 CB B:ASP364 4.4 61.9 1.0 N B:LEU371 4.4 49.0 1.0 N B:ASP366 4.4 65.3 1.0 N B:VAL370 4.4 48.9 1.0 CB B:ASP366 4.4 56.7 1.0 CA B:VAL370 4.5 59.4 1.0 C B:ASP364 4.6 52.6 1.0 N B:GLY367 4.6 72.1 1.0 CB B:SER372 4.6 46.0 1.0 N B:VAL365 4.6 60.6 1.0 C B:LEU371 4.7 52.2 1.0 CA B:ASP366 4.8 68.4 1.0 CA B:ASP368 4.8 68.2 1.0 CA B:SER372 5.0 38.2 1.0

N.Wlodarchak, F.Guo, K.A.Satyshur, L.Jiang, P.D.Jeffrey, T.Sun, V.Stanevich, M.C.Mumby, Y.Xing. Structure of the Ca(2+)-Dependent PP2A Heterotrimer and Insights Into CDC6 Dephosphorylation. Cell Res. V. 23 931 2013.
ISSN: ISSN 1001-0602
PubMed: 23752926
DOI: 10.1038/CR.2013.77