Calcium in PDB 4i9q: Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase

Enzymatic activity of Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase

All present enzymatic activity of Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase:
2.7.7.7;

Protein crystallography data

The structure of Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase, PDB code: 4i9q was solved by K.E.Guja, A.Jacewicz, A.Trzemecka, D.Plochocka, E.Yakubovskaya, A.Bebenek, M.Garcia-Diaz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 38.40 / 2.30
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 73.781, 119.412, 146.016, 90.00, 90.26, 90.00
R / Rfree (%) 25.2 / 28.7

Other elements in 4i9q:

The structure of Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase also contains other interesting chemical elements:

Sodium (Na) 2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase (pdb code 4i9q). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase, PDB code: 4i9q:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 4i9q

Go back to Calcium Binding Sites List in 4i9q
Calcium binding site 1 out of 2 in the Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca1003

b:51.7
occ:1.00
O2B A:XG41001 2.3 59.9 1.0
OD2 A:ASP411 2.4 37.2 1.0
O3G A:XG41001 2.5 52.8 1.0
O A:HOH1315 2.7 54.6 1.0
O A:LEU412 2.9 28.4 1.0
O A:HOH1314 3.1 36.2 1.0
PB A:XG41001 3.4 36.2 1.0
PG A:XG41001 3.5 49.6 1.0
O2G A:XG41001 3.6 50.5 1.0
N3A A:XG41001 3.6 50.4 1.0
CG A:ASP411 3.7 40.1 1.0
O3B A:XG41001 3.8 58.7 1.0
NA A:NA1004 3.9 44.1 1.0
C A:LEU412 4.0 24.4 1.0
OD2 A:ASP623 4.1 33.9 1.0
CA A:THR413 4.3 28.5 1.0
N A:SER414 4.3 23.1 1.0
O A:HOH1316 4.4 21.8 1.0
OD1 A:ASP411 4.5 47.9 1.0
CB A:ASP411 4.5 33.5 1.0
N A:THR413 4.6 26.9 1.0
O1B A:XG41001 4.6 39.7 1.0
C A:THR413 4.8 26.9 1.0
O1G A:XG41001 4.9 51.0 1.0
C A:ASP411 5.0 29.3 1.0

Calcium binding site 2 out of 2 in 4i9q

Go back to Calcium Binding Sites List in 4i9q
Calcium binding site 2 out of 2 in the Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Ternary Complex of the D714A Mutant of RB69 Dna Polymerase within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca1003

b:78.5
occ:1.00
O2B B:XG41001 2.4 39.0 1.0
O1G B:XG41001 2.5 34.2 1.0
O B:LEU412 2.9 38.7 1.0
OD2 B:ASP411 2.9 51.4 1.0
PG B:XG41001 3.6 33.0 1.0
O B:HOH1233 3.6 37.5 1.0
PB B:XG41001 3.7 37.6 1.0
C B:LEU412 3.9 37.4 1.0
CG B:ASP411 3.9 50.8 1.0
O3G B:XG41001 3.9 32.8 1.0
CA B:THR413 4.1 35.7 1.0
N3A B:XG41001 4.1 27.7 1.0
O3B B:XG41001 4.1 49.6 1.0
NA B:NA1004 4.2 59.9 1.0
N B:SER414 4.2 32.2 1.0
CB B:ASP411 4.4 46.4 1.0
N B:THR413 4.4 37.9 1.0
O B:HOH1234 4.5 30.7 1.0
OD2 B:ASP623 4.7 39.7 1.0
C B:THR413 4.7 35.7 1.0
OD1 B:ASP411 4.8 53.1 1.0
N B:LEU412 4.9 39.1 1.0
C B:ASP411 4.9 41.2 1.0
O1B B:XG41001 4.9 37.7 1.0
O2G B:XG41001 5.0 36.7 1.0
O B:ASP411 5.0 41.4 1.0
CA B:LEU412 5.0 37.9 1.0

Reference:

A.Jacewicz, A.Trzemecka, K.E.Guja, D.Plochocka, E.Yakubovskaya, A.Bebenek, M.Garcia-Diaz. A Remote Palm Domain Residue of RB69 Dna Polymerase Is Critical For Enzyme Activity and Influences the Conformation of the Active Site. Plos One V. 8 76700 2013.
ISSN: ESSN 1932-6203
PubMed: 24116139
DOI: 10.1371/JOURNAL.PONE.0076700
Page generated: Sat Dec 12 04:51:32 2020

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