Calcium in PDB 4jws: Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex

All present enzymatic activity of Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex:
1.14.15.1;

The structure of Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex, PDB code: 4jws was solved by S.M.Tripathi, H.Li, T.L.Poulos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	54.80 / 2.15
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	57.590, 106.720, 88.700, 90.00, 107.91, 90.00
R / Rfree (%)	17.5 / 22.5

The structure of Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex also contains other interesting chemical elements:

Iron

(Fe)

6 atoms

The binding sites of Calcium atom in the Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex (pdb code 4jws). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex, PDB code: 4jws:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:54.7 occ:1.00 OE2 A:GLU198 2.3 68.2 1.0 OD2 A:ASP202 2.4 66.9 1.0 OD1 A:ASP202 2.4 58.2 1.0 O B:ALA36 2.7 54.0 1.0 CG A:ASP202 2.8 57.2 1.0 CD A:GLU198 3.3 67.9 1.0 C B:ALA36 3.5 54.7 1.0 CG A:GLU198 4.0 59.3 1.0 CA B:ALA36 4.2 54.2 1.0 OE1 A:GLU198 4.2 60.3 1.0 NZ B:LYS266 4.2 76.5 1.0 N B:GLY37 4.3 48.6 1.0 CB A:ASP202 4.3 57.4 1.0 OE2 B:GLU40 4.5 62.7 1.0 CA B:GLY37 4.5 48.8 1.0 CD B:GLU40 4.7 59.2 1.0 OE1 B:GLU40 4.8 53.7 1.0 O B:SER35 4.9 53.5 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Cytochrome P450CAM-Putidaredoxin Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca502 b:54.9 occ:1.00 OE2 B:GLU198 2.4 81.0 1.0 OD1 B:ASP202 2.5 56.2 1.0 OD2 B:ASP202 2.7 54.8 1.0 CG B:ASP202 2.9 57.3 1.0 CD B:GLU198 3.4 82.0 1.0 CG B:GLU198 4.2 73.1 1.0 OE1 B:GLU198 4.2 79.4 1.0 CB B:ASP202 4.4 46.2 1.0

S.Tripathi, H.Li, T.L.Poulos. Structural Basis For Effector Control and Redox Partner Recognition in Cytochrome P450. Science V. 340 1227 2013.
ISSN: ISSN 0036-8075
PubMed: 23744947
DOI: 10.1126/SCIENCE.1235797