Calcium in PDB 4l41: Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase

All present enzymatic activity of Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase:
2.4.1.22;

The structure of Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase, PDB code: 4l41 was solved by B.Ramakrishnan, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.52 / 2.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	62.390, 65.210, 71.720, 90.00, 114.45, 90.00
R / Rfree (%)	18.8 / 23.7

The binding sites of Calcium atom in the Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase (pdb code 4l41). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase, PDB code: 4l41:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca201 b:28.3 occ:1.00 O A:ASP85 2.3 26.6 1.0 O A:LYS80 2.3 28.8 1.0 OD1 A:ASP83 2.3 35.4 1.0 OD1 A:ASP88 2.5 35.5 1.0 OD1 A:ASP89 2.5 33.3 1.0 O A:HOH302 2.7 29.2 1.0 OD2 A:ASP83 2.9 26.8 1.0 CG A:ASP83 3.0 32.9 1.0 CG A:ASP88 3.4 28.2 1.0 C A:ASP85 3.5 28.8 1.0 C A:LYS80 3.5 30.8 1.0 OD2 A:ASP88 3.7 30.9 1.0 CG A:ASP89 3.7 29.8 1.0 CA A:PHE81 4.2 30.6 1.0 CA A:ILE86 4.3 27.6 1.0 N A:PHE81 4.3 29.7 1.0 N A:ILE86 4.3 24.5 1.0 N A:ASP89 4.4 29.1 1.0 CD1 A:PHE81 4.4 28.0 1.0 CA A:ASP85 4.4 28.9 1.0 CB A:ASP83 4.5 25.6 1.0 OD2 A:ASP89 4.5 33.0 1.0 CA A:LYS80 4.5 26.2 1.0 CB A:LYS80 4.6 26.0 1.0 N A:ASP85 4.6 31.2 1.0 CB A:ASP85 4.6 23.9 1.0 N A:ASP88 4.7 26.5 1.0 CB A:ASP89 4.7 26.5 1.0 CB A:ASP88 4.8 20.1 1.0 N A:ASP83 4.8 27.8 1.0 C A:ILE86 4.9 26.7 1.0 N A:THR87 4.9 23.1 1.0 C A:PHE81 4.9 33.5 1.0

Calcium binding site 2 out of 2 in the Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Human Lactose Synthase: A 2:1 Complex Between Human Alpha-Lactalbumin and Human BETA1,4-Galactosyltransferase within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca201 b:37.9 occ:1.00 OD1 B:ASP89 2.3 31.2 1.0 OD1 B:ASP83 2.3 45.2 1.0 O B:ASP85 2.4 32.3 1.0 O B:LYS80 2.4 33.4 1.0 OD1 B:ASP88 3.0 40.6 1.0 CG B:ASP83 3.2 44.4 1.0 CG B:ASP89 3.3 27.2 1.0 C B:ASP85 3.4 37.7 1.0 C B:LYS80 3.5 34.1 1.0 OD2 B:ASP89 3.6 23.1 1.0 CA B:PHE81 3.6 31.2 1.0 O B:ASP83 3.6 43.5 1.0 OD2 B:ASP83 3.8 55.9 1.0 N B:ASP83 3.8 34.6 1.0 C B:PHE81 3.8 35.8 1.0 N B:LEU82 4.0 41.9 1.0 CG B:ASP88 4.0 37.1 1.0 N B:PHE81 4.0 30.3 1.0 CA B:ILE86 4.0 29.0 1.0 N B:ILE86 4.1 34.5 1.0 C B:ASP83 4.1 37.5 1.0 CB B:ASP83 4.1 33.4 1.0 OD2 B:ASP88 4.1 34.8 1.0 CA B:ASP83 4.2 32.7 1.0 N B:ASP85 4.4 37.3 1.0 CA B:ASP85 4.4 39.2 1.0 O B:PHE81 4.5 31.3 1.0 CB B:ASP89 4.6 27.5 1.0 CD1 B:PHE81 4.6 27.9 1.0 C B:LEU82 4.6 36.5 1.0 N B:ASP89 4.7 24.4 1.0 CA B:LYS80 4.8 29.0 1.0 CA B:LEU82 4.8 34.2 1.0 CB B:PHE81 4.8 29.5 1.0 CG2 B:ILE86 4.9 20.8 1.0 CB B:ASP85 4.9 36.4 1.0 C B:ILE86 4.9 27.5 1.0

B.Ramakrishnan, P.K.Qasba. Crystal Structure of Human Lactose Synthase Forms A Novel 1:2 Complex Between BETA1,4GALACTOSYLTRANSFERASE 1 and Alpha-Lactalbumin Proteins To Be Published.