Calcium in PDB 4lhk: Structure of the N-Terminal Domain of the Lg-FLO1 Adhesin (N-Lg-FLO1P) From the Yeast Saccharomyces Pastorianus, in Complex with Calcium and Alpha-1,2-Mannobiose

The structure of Structure of the N-Terminal Domain of the Lg-FLO1 Adhesin (N-Lg-FLO1P) From the Yeast Saccharomyces Pastorianus, in Complex with Calcium and Alpha-1,2-Mannobiose, PDB code: 4lhk was solved by F.S.Ielasi, R.G.Willaert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.13 / 1.73
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	38.618, 85.476, 68.240, 90.00, 93.72, 90.00
R / Rfree (%)	13.7 / 19.3

The binding sites of Calcium atom in the Structure of the N-Terminal Domain of the Lg-FLO1 Adhesin (N-Lg-FLO1P) From the Yeast Saccharomyces Pastorianus, in Complex with Calcium and Alpha-1,2-Mannobiose (pdb code 4lhk). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of the N-Terminal Domain of the Lg-FLO1 Adhesin (N-Lg-FLO1P) From the Yeast Saccharomyces Pastorianus, in Complex with Calcium and Alpha-1,2-Mannobiose, PDB code: 4lhk:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of the N-Terminal Domain of the Lg-FLO1 Adhesin (N-Lg-FLO1P) From the Yeast Saccharomyces Pastorianus, in Complex with Calcium and Alpha-1,2-Mannobiose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of the N-Terminal Domain of the Lg-FLO1 Adhesin (N-Lg-FLO1P) From the Yeast Saccharomyces Pastorianus, in Complex with Calcium and Alpha-1,2-Mannobiose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca303 b:15.1 occ:1.00 OD1 A:ASN197 2.3 16.0 1.0 O A:LYS199 2.3 14.0 1.0 OD1 A:ASP133 2.3 15.6 1.0 O A:LEU201 2.4 15.0 1.0 OD2 A:ASP134 2.5 13.9 1.0 O4 A:MAN301 2.5 13.5 0.9 O3 A:MAN301 2.5 13.4 0.9 CG A:ASP133 3.3 15.0 1.0 C3 A:MAN301 3.3 14.8 0.9 CG A:ASP134 3.3 12.7 1.0 CG A:ASN197 3.4 13.0 1.0 C4 A:MAN301 3.4 13.2 0.9 C A:LYS199 3.5 13.0 1.0 C A:LEU201 3.5 15.3 1.0 OD2 A:ASP133 3.7 14.4 1.0 OD1 A:ASP134 3.7 18.5 1.0 N A:LEU201 3.8 15.9 1.0 ND2 A:ASN197 3.9 14.6 1.0 N A:LYS199 4.0 12.9 1.0 C A:ALA200 4.1 15.7 1.0 CA A:LEU201 4.2 16.1 1.0 C A:ASP133 4.3 14.5 1.0 O A:ASP133 4.3 17.3 1.0 CA A:LYS199 4.3 14.8 1.0 N A:ALA198 4.4 15.8 1.0 N A:ASP134 4.4 14.1 1.0 O A:ALA200 4.4 14.7 1.0 N A:ALA200 4.4 14.2 1.0 CB A:ASP133 4.5 14.5 1.0 CB A:ASP134 4.5 14.4 1.0 N A:ALA202 4.6 13.0 1.0 CB A:ASN197 4.6 13.1 1.0 CA A:ALA200 4.6 13.7 1.0 CA A:ASN197 4.7 12.6 1.0 CB A:LEU201 4.7 19.9 1.0 C2 A:MAN301 4.7 17.2 0.9 C5 A:MAN301 4.8 17.6 0.9 CG A:LYS199 4.8 22.1 1.0 CB A:ALA200 4.8 13.9 1.0 CA A:ASP133 4.9 17.0 1.0 CA A:ALA202 4.9 13.8 1.0 CA A:ASP134 4.9 14.5 1.0 C A:ASN197 5.0 13.9 1.0

Calcium binding site 2 out of 2 in the Structure of the N-Terminal Domain of the Lg-FLO1 Adhesin (N-Lg-FLO1P) From the Yeast Saccharomyces Pastorianus, in Complex with Calcium and Alpha-1,2-Mannobiose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of the N-Terminal Domain of the Lg-FLO1 Adhesin (N-Lg-FLO1P) From the Yeast Saccharomyces Pastorianus, in Complex with Calcium and Alpha-1,2-Mannobiose within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca303 b:16.1 occ:1.00 O B:LYS199 2.3 14.8 1.0 OD1 B:ASN197 2.3 16.8 1.0 O B:LEU201 2.3 14.2 1.0 OD1 B:ASP133 2.4 16.0 1.0 OD2 B:ASP134 2.4 15.9 1.0 O4 B:MAN301 2.5 13.3 0.9 O3 B:MAN301 2.7 18.3 0.9 CG B:ASP133 3.3 16.2 1.0 CG B:ASP134 3.3 14.2 1.0 C4 B:MAN301 3.4 15.8 0.9 C3 B:MAN301 3.4 16.9 0.9 CG B:ASN197 3.4 13.4 1.0 C B:LEU201 3.5 15.8 1.0 C B:LYS199 3.5 14.0 1.0 OD2 B:ASP133 3.6 16.6 1.0 OD1 B:ASP134 3.6 16.7 1.0 N B:LEU201 3.8 15.5 1.0 N B:LYS199 4.0 14.8 1.0 ND2 B:ASN197 4.0 14.5 1.0 C B:ALA200 4.1 15.3 1.0 CA B:LEU201 4.2 14.0 1.0 C B:ASP133 4.3 17.2 1.0 CA B:LYS199 4.4 16.6 1.0 O B:ASP133 4.4 16.1 1.0 N B:ALA198 4.4 14.8 1.0 N B:ASP134 4.4 16.6 1.0 N B:ALA200 4.5 13.9 1.0 CB B:ASP134 4.5 14.7 1.0 O B:ALA200 4.5 14.9 1.0 CB B:ASP133 4.5 14.9 1.0 N B:ALA202 4.6 13.9 1.0 CB B:ASN197 4.6 14.1 1.0 CA B:ALA200 4.7 14.6 1.0 CA B:ASN197 4.7 12.4 1.0 C5 B:MAN301 4.7 17.6 0.9 CG B:LYS199 4.8 21.9 1.0 CA B:ALA202 4.8 14.5 1.0 C2 B:MAN301 4.9 19.3 0.9 CB B:ALA200 4.9 14.5 1.0 CA B:ASP133 4.9 17.7 1.0 CA B:ASP134 4.9 14.5 1.0 CB B:LEU201 4.9 17.7 1.0

K.Goossens, F.S.Ielasi, I.Nookaew, I.Stals, L.Alonso-Sarduy, L.Daenen, S.E.Van Mulders, R.G.E.Van Eijsden, C.Stassen, V.Siewers, F.R.Delvaux, S.Kasas, J.Nielsen, B.Devreese, R.G.Willaert. Molecular Mechanism of Flocculation Self-Recognition in Yeast and Its Role in Mating and Survival To Be Published.