Calcium in PDB 4lq0: Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna.

The structure of Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna., PDB code: 4lq0 was solved by J.Chik, B.Shen, B.Stoddard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.34 / 2.68
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.553, 71.959, 168.648, 90.00, 90.00, 90.00
R / Rfree (%)	19.7 / 27.1

The binding sites of Calcium atom in the Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna. (pdb code 4lq0). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna., PDB code: 4lq0:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:42.0 occ:1.00 OP1 C:DC15 2.1 35.0 1.0 O A:HOH509 2.3 43.3 1.0 OP2 B:DG15 2.3 29.4 1.0 OE1 A:GLU22 2.3 39.2 1.0 O A:GLY180 2.4 34.6 1.0 C A:GLY180 3.5 33.7 1.0 CD A:GLU22 3.5 41.1 1.0 P C:DC15 3.6 34.5 1.0 P B:DG15 3.7 32.4 1.0 CA A:GLY180 3.9 32.9 1.0 OE2 A:GLU22 4.1 44.0 1.0 CA A:CA403 4.1 79.0 1.0 O3' C:DA14 4.2 36.2 1.0 O5' B:DG15 4.2 32.4 1.0 C5' B:DG15 4.3 30.9 1.0 CE A:MET49 4.3 39.5 1.0 C5' C:DC15 4.4 35.1 1.0 O A:GLU179 4.4 39.2 1.0 O5' C:DC15 4.5 35.9 1.0 C4' C:DC15 4.5 35.3 1.0 OP2 C:DC15 4.5 37.0 1.0 OP1 B:DG15 4.6 32.4 1.0 NZ A:LYS264 4.6 41.0 1.0 N A:GLU181 4.7 31.1 1.0 CG A:GLU22 4.7 38.0 1.0 O3' B:DT14 4.8 30.8 1.0 OE1 A:GLU181 4.9 41.1 1.0 N A:GLY180 5.0 33.0 1.0

Calcium binding site 2 out of 3 in the Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:36.1 occ:1.00 OP2 C:DT16 2.2 33.5 1.0 O A:HOH507 2.2 29.1 1.0 OP1 B:DT14 2.3 39.0 1.0 O A:HOH508 2.3 36.7 1.0 O A:ALA21 2.3 34.4 1.0 OE2 A:GLU181 2.5 35.1 1.0 P C:DT16 3.4 34.7 1.0 C A:ALA21 3.5 34.4 1.0 CD A:GLU181 3.5 37.5 1.0 P B:DT14 3.7 39.8 1.0 CA A:CA403 3.8 79.0 1.0 OE1 A:GLU181 3.9 41.1 1.0 NZ A:LYS106 3.9 47.9 1.0 C5' C:DT16 3.9 31.1 1.0 O5' C:DT16 4.0 31.0 1.0 NE2 A:GLN208 4.0 41.0 1.0 CA A:ALA21 4.1 34.5 1.0 OP1 C:DT16 4.2 36.9 1.0 O3' B:DA13 4.3 39.3 1.0 OE2 A:GLU22 4.3 44.0 1.0 O A:ASP20 4.4 39.4 1.0 C5' B:DT14 4.5 34.5 1.0 N A:GLU22 4.6 34.1 1.0 C4' B:DT14 4.6 33.6 1.0 OP2 B:DT14 4.6 41.6 1.0 O3' C:DC15 4.6 35.0 1.0 O5' B:DT14 4.7 35.5 1.0 OE1 A:GLN208 4.7 41.8 1.0 CG A:GLU181 4.8 38.2 1.0 C4' C:DT16 4.8 32.3 1.0 CD A:GLN208 4.8 41.9 1.0 CA A:GLU22 4.9 35.1 1.0 CB A:ALA21 4.9 34.0 1.0

Calcium binding site 3 out of 3 in the Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of the I-Ltrwi Laglidadg Homing Endonuclease Bound to Target Dna. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:79.0 occ:1.00 OP2 C:DT16 2.3 33.5 1.0 OE2 A:GLU22 2.4 44.0 1.0 O3' B:DT14 2.4 30.8 1.0 OP2 B:DG15 2.7 29.4 1.0 O3' C:DC15 2.7 35.0 1.0 OE1 A:GLU181 2.8 41.1 1.0 P C:DT16 3.1 34.7 1.0 CD A:GLU181 3.1 37.5 1.0 P B:DG15 3.1 32.4 1.0 C3' B:DT14 3.3 32.2 1.0 CD A:GLU22 3.3 41.1 1.0 OE2 A:GLU181 3.4 35.1 1.0 OE1 A:GLU22 3.5 39.2 1.0 C4' B:DT14 3.7 33.6 1.0 CA A:CA402 3.8 36.1 1.0 O A:ALA21 3.8 34.4 1.0 C3' C:DC15 3.8 35.5 1.0 O5' B:DG15 4.0 32.4 1.0 CG A:GLU181 4.0 38.2 1.0 O A:GLY180 4.0 34.6 1.0 CA A:CA401 4.1 42.0 1.0 OP1 C:DT16 4.2 36.9 1.0 C4' C:DC15 4.2 35.3 1.0 O5' C:DT16 4.2 31.0 1.0 OP1 B:DG15 4.4 32.4 1.0 C A:ALA21 4.6 34.4 1.0 C5' B:DT14 4.6 34.5 1.0 OP1 B:DT14 4.6 39.0 1.0 C A:GLY180 4.6 33.7 1.0 C2' B:DT14 4.7 34.2 1.0 CG A:GLU22 4.7 38.0 1.0 O4' B:DT14 4.8 35.6 1.0 CA A:GLU181 5.0 30.8 1.0 CB A:ALA21 5.0 34.0 1.0

J.Chik, B.Shen, B.Stoddard. Structural Comparisons of Laglidadg Homing Endonucleases. To Be Published.