Calcium in PDB 4lt6: Crystal Structure of Human Poly(A) Polymerase Gamma

All present enzymatic activity of Crystal Structure of Human Poly(A) Polymerase Gamma:
2.7.7.19;

The structure of Crystal Structure of Human Poly(A) Polymerase Gamma, PDB code: 4lt6 was solved by Q.Yang, L.Nausch, G.Martin, W.Keller, S.Doublie, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.88 / 2.79
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	68.870, 89.920, 201.630, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 25.7

The binding sites of Calcium atom in the Crystal Structure of Human Poly(A) Polymerase Gamma (pdb code 4lt6). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Human Poly(A) Polymerase Gamma, PDB code: 4lt6:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Human Poly(A) Polymerase Gamma


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Human Poly(A) Polymerase Gamma within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca602 b:31.7 occ:1.00 O2G A:3AT601 2.3 29.3 1.0 OD2 A:ASP114 2.4 21.3 1.0 OD1 A:ASP112 2.4 23.6 1.0 O1B A:3AT601 2.5 28.4 1.0 O1A A:3AT601 2.9 32.4 1.0 CG A:ASP114 3.4 21.4 1.0 CG A:ASP112 3.4 26.2 1.0 PG A:3AT601 3.6 24.5 1.0 PB A:3AT601 3.6 27.6 1.0 O A:HOH722 3.8 17.5 1.0 OD2 A:ASP112 3.8 31.8 1.0 OD1 A:ASP114 3.8 17.6 1.0 C5' A:3AT601 4.0 26.2 1.0 PA A:3AT601 4.0 37.6 1.0 O3B A:3AT601 4.0 23.8 1.0 O3G A:3AT601 4.2 26.8 1.0 O3A A:3AT601 4.2 24.8 1.0 O A:ASP112 4.2 16.1 1.0 O5' A:3AT601 4.4 28.5 1.0 C A:ASP112 4.6 14.4 1.0 CB A:ASP114 4.6 16.1 1.0 CB A:SER101 4.7 12.6 1.0 N A:ASP114 4.8 13.7 1.0 CB A:ASP112 4.8 16.8 1.0 C4' A:3AT601 4.8 25.9 1.0 C3' A:3AT601 4.8 24.5 1.0 OG A:SER101 4.8 18.5 1.0 O1G A:3AT601 4.8 20.9 1.0 N A:SER101 4.9 12.8 1.0 O2B A:3AT601 5.0 21.9 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Human Poly(A) Polymerase Gamma


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Human Poly(A) Polymerase Gamma within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca602 b:34.0 occ:1.00 O1G B:3AT601 2.3 24.2 1.0 O1B B:3AT601 2.4 27.7 1.0 OD2 B:ASP114 2.5 29.7 1.0 OD1 B:ASP112 2.6 31.0 1.0 O1A B:3AT601 2.8 37.5 1.0 CG B:ASP114 3.5 22.3 1.0 PB B:3AT601 3.6 32.5 1.0 PG B:3AT601 3.6 28.8 1.0 CG B:ASP112 3.7 31.8 1.0 PA B:3AT601 3.9 46.6 1.0 OD1 B:ASP114 4.0 29.2 1.0 O3A B:3AT601 4.0 31.4 1.0 O3B B:3AT601 4.0 25.0 1.0 C5' B:3AT601 4.1 33.3 1.0 O3G B:3AT601 4.1 23.3 1.0 OD2 B:ASP112 4.1 41.7 1.0 O B:ASP112 4.3 21.6 1.0 O5' B:3AT601 4.5 36.8 1.0 CB B:ASP114 4.7 17.4 1.0 C B:ASP112 4.8 22.3 1.0 OG B:SER101 4.8 24.6 1.0 CB B:SER101 4.8 15.3 1.0 O2G B:3AT601 4.8 24.7 1.0 N B:ASP114 4.9 27.7 1.0 N B:SER101 4.9 19.6 1.0 O2B B:3AT601 4.9 20.8 1.0 CB B:ASP112 5.0 23.5 1.0

Q.Yang, L.W.Nausch, G.Martin, W.Keller, S.Doublie. Crystal Structure of Human Poly(A) Polymerase Gamma Reveals A Conserved Catalytic Core For Canonical Poly(A) Polymerases. J.Mol.Biol. V. 426 43 2014.
ISSN: ISSN 0022-2836
PubMed: 24076191
DOI: 10.1016/J.JMB.2013.09.025