Calcium in PDB 4ol7: Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature

All present enzymatic activity of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature:
3.1.31.1;

The structure of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature, PDB code: 4ol7 was solved by P.C.Bell-Upp, J.L.Schlessman, B.Garcia-Moreno E., with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.47 / 1.67
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	47.145, 54.159, 108.125, 90.00, 90.00, 90.00
R / Rfree (%)	15.8 / 19.1

The binding sites of Calcium atom in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature (pdb code 4ol7). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature, PDB code: 4ol7:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca202 b:9.2 occ:1.00 O A:HOH370 2.3 20.0 1.0 O A:HOH312 2.4 8.4 1.0 O A:HOH307 2.4 10.7 1.0 OD1 A:ASP21 2.4 9.3 1.0 OD2 A:ASP19 2.4 9.4 1.0 O A:HOH310 2.4 10.0 1.0 OD1 A:ASP19 2.9 8.8 1.0 CG A:ASP19 3.0 8.4 1.0 CG A:ASP21 3.3 8.4 1.0 OD2 A:ASP21 3.6 8.0 1.0 OE2 B:GLU135 4.2 11.3 1.0 N A:ASP21 4.3 7.3 1.0 O5P A:THP201 4.3 7.7 1.0 CB A:ASP19 4.4 8.1 1.0 O A:HOH343 4.4 14.6 1.0 OG1 A:THR22 4.5 9.2 0.6 O B:HOH410 4.5 24.3 1.0 CB A:ASP21 4.6 7.9 1.0 OG1 A:THR22 4.6 9.1 0.4 O A:HOH316 4.7 11.5 1.0 O A:HOH390 4.7 26.4 1.0 CG2 A:THR22 4.7 8.8 0.4 CA A:ASP21 4.7 7.5 1.0 O6P A:THP201 4.7 7.7 1.0 O A:HOH383 4.8 21.6 1.0 N A:THR22 4.8 7.8 1.0 C A:ASP21 4.8 7.8 1.0 OG1 A:THR41 4.9 11.7 1.0 N A:GLY20 4.9 7.3 1.0 OE2 A:GLU109 4.9 7.5 1.0 O A:HOH347 4.9 19.8 1.0 P2 A:THP201 5.0 7.6 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca202 b:9.7 occ:1.00 O B:HOH322 2.3 11.0 1.0 O B:HOH347 2.3 18.3 1.0 OD2 B:ASP19 2.3 9.1 1.0 O B:HOH304 2.4 9.7 1.0 O B:HOH314 2.4 11.1 1.0 OD1 B:ASP21 2.4 7.2 1.0 OD1 B:ASP19 2.9 9.4 1.0 CG B:ASP19 3.0 9.2 1.0 CG B:ASP21 3.3 7.4 1.0 O B:HOH418 3.5 35.4 1.0 OD2 B:ASP21 3.6 7.7 1.0 N B:ASP21 4.3 7.5 1.0 O5P B:THP201 4.3 8.4 1.0 OG1 B:THR22 4.3 8.5 0.5 CB B:ASP19 4.4 9.2 1.0 O B:HOH353 4.5 15.8 1.0 CB B:ASP21 4.6 7.3 1.0 O B:HOH313 4.6 10.5 1.0 OG1 B:THR22 4.7 9.1 0.5 CG2 B:THR22 4.7 8.5 0.5 O6P B:THP201 4.7 9.0 1.0 CA B:ASP21 4.7 7.1 1.0 OG1 B:THR41 4.8 12.8 1.0 N B:THR22 4.8 7.4 1.0 C B:ASP21 4.8 7.1 1.0 N B:GLY20 4.9 8.7 1.0 OE2 B:GLU109 4.9 10.4 1.0 O B:HOH331 5.0 15.9 1.0 P2 B:THP201 5.0 8.6 1.0 NE B:ARG35 5.0 7.4 1.0

P.C.Bell-Upp, J.L.Sorenson, A.C.Robinson, E.L.Wheeler, M.Peck, I.Caturegli, J.L.Schlessman, B.Garcia-Moreno E.. Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs V66E A109E at Cryogenic Temperature To Be Published.