Calcium in PDB 4op5: Crystal Structure of Stabilized Tem-1 Beta-Lactamase Variant V.13 Carrying R164S Mutation

The structure of Crystal Structure of Stabilized Tem-1 Beta-Lactamase Variant V.13 Carrying R164S Mutation, PDB code: 4op5 was solved by E.Dellus-Gur, M.Elias, J.S.Fraser, D.S.Tawfik, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.15 / 1.05
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	153.600, 46.100, 34.500, 90.00, 93.30, 90.00
R / Rfree (%)	13.1 / 15.7

The binding sites of Calcium atom in the Crystal Structure of Stabilized Tem-1 Beta-Lactamase Variant V.13 Carrying R164S Mutation (pdb code 4op5). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Stabilized Tem-1 Beta-Lactamase Variant V.13 Carrying R164S Mutation, PDB code: 4op5:

Calcium binding site 1 out of 1 in the Crystal Structure of Stabilized Tem-1 Beta-Lactamase Variant V.13 Carrying R164S Mutation


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Stabilized Tem-1 Beta-Lactamase Variant V.13 Carrying R164S Mutation within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:5.5 occ:0.50 O A:HOH408 2.2 8.5 1.0 OE2 A:GLU36 2.3 7.1 1.0 O A:HOH401 2.3 9.0 1.0 CD A:GLU36 3.4 6.9 1.0 CG A:GLU36 3.9 7.2 1.0 OD1 A:ASP37 4.1 13.2 1.0 O A:HOH494 4.1 14.4 1.0 NH2 A:ARG60 4.2 7.5 1.0 OE1 A:GLU36 4.4 6.8 1.0 CB A:GLU36 4.5 7.3 1.0 O A:HOH496 4.5 11.3 1.0 CZ A:PHE59 4.9 9.1 1.0

E.Dellus-Gur, M.Elias, E.Caselli, F.Pratti, J.S.Fraser, D.S.Tawfik. Negative Epistasis in Enzyme Evolution the Thin Line Between Conformational Freedom and Anarchy To Be Published.