Calcium in PDB 4pl8: Structure of Rabbit Skeletal Muscle Actin in Complex with A Hybrid Peptide Comprising Thymosin BETA4 and the Lysine-Rich Region of Cordon-Bleu

The structure of Structure of Rabbit Skeletal Muscle Actin in Complex with A Hybrid Peptide Comprising Thymosin BETA4 and the Lysine-Rich Region of Cordon-Bleu, PDB code: 4pl8 was solved by B.Xue, R.C.Robinson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.90 / 2.00
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	93.640, 93.640, 206.274, 90.00, 90.00, 90.00
R / Rfree (%)	17.1 / 20.7

The binding sites of Calcium atom in the Structure of Rabbit Skeletal Muscle Actin in Complex with A Hybrid Peptide Comprising Thymosin BETA4 and the Lysine-Rich Region of Cordon-Bleu (pdb code 4pl8). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of Rabbit Skeletal Muscle Actin in Complex with A Hybrid Peptide Comprising Thymosin BETA4 and the Lysine-Rich Region of Cordon-Bleu, PDB code: 4pl8:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of Rabbit Skeletal Muscle Actin in Complex with A Hybrid Peptide Comprising Thymosin BETA4 and the Lysine-Rich Region of Cordon-Bleu


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Rabbit Skeletal Muscle Actin in Complex with A Hybrid Peptide Comprising Thymosin BETA4 and the Lysine-Rich Region of Cordon-Bleu within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:16.8 occ:1.00 O1B A:ATP401 2.2 18.8 1.0 O A:HOH564 2.4 14.8 1.0 O2G A:ATP401 2.4 13.5 1.0 O A:HOH563 2.4 15.2 1.0 O A:HOH568 2.5 18.7 1.0 O A:HOH566 2.5 13.7 1.0 O A:HOH567 2.7 14.9 1.0 PG A:ATP401 3.5 12.6 1.0 PB A:ATP401 3.6 13.6 1.0 O3B A:ATP401 3.9 13.0 1.0 O3G A:ATP401 3.9 18.1 1.0 O A:HOH569 4.2 14.0 1.0 OE1 A:GLN137 4.2 16.2 1.0 O3A A:ATP401 4.2 20.7 1.0 O1A A:ATP401 4.3 17.9 1.0 CA A:GLY13 4.3 16.0 1.0 NZ A:LYS18 4.4 19.5 1.0 CD A:GLN137 4.5 19.4 1.0 O A:HOH598 4.5 20.8 1.0 OD2 A:ASP11 4.6 18.3 1.0 OD1 A:ASP11 4.7 17.7 1.0 OD1 A:ASP154 4.7 24.9 1.0 PA A:ATP401 4.8 17.3 1.0 O A:HOH618 4.8 35.9 1.0 O1G A:ATP401 4.8 18.3 1.0 O2B A:ATP401 4.8 16.7 1.0 NE2 A:GLN137 4.9 13.3 1.0 CG A:GLN137 4.9 12.6 1.0 OD2 A:ASP154 5.0 30.6 1.0

Calcium binding site 2 out of 2 in the Structure of Rabbit Skeletal Muscle Actin in Complex with A Hybrid Peptide Comprising Thymosin BETA4 and the Lysine-Rich Region of Cordon-Bleu


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of Rabbit Skeletal Muscle Actin in Complex with A Hybrid Peptide Comprising Thymosin BETA4 and the Lysine-Rich Region of Cordon-Bleu within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca402 b:19.1 occ:1.00 O2G B:ATP401 2.3 18.9 1.0 O1B B:ATP401 2.4 19.3 1.0 O B:HOH639 2.4 19.9 1.0 O B:HOH554 2.5 19.2 1.0 O B:HOH551 2.5 20.3 1.0 O B:HOH552 2.6 20.6 1.0 O B:HOH550 2.6 20.2 1.0 PG B:ATP401 3.5 22.8 1.0 PB B:ATP401 3.6 19.6 1.0 O3G B:ATP401 3.9 24.8 1.0 O3B B:ATP401 3.9 20.5 1.0 O B:HOH656 4.1 22.7 1.0 OE1 B:GLN137 4.1 25.9 1.0 O3A B:ATP401 4.3 19.0 1.0 CA B:GLY13 4.3 24.3 1.0 O1A B:ATP401 4.4 26.2 1.0 NZ B:LYS18 4.4 24.0 1.0 CD B:GLN137 4.4 25.2 1.0 O B:HOH580 4.6 29.0 1.0 OD2 B:ASP11 4.6 22.9 1.0 OD1 B:ASP154 4.6 24.8 1.0 O B:HOH640 4.6 33.5 1.0 PA B:ATP401 4.8 21.6 1.0 OD1 B:ASP11 4.8 22.7 1.0 O1G B:ATP401 4.8 20.9 1.0 O2B B:ATP401 4.9 19.9 1.0 NE2 B:GLN137 4.9 20.9 1.0 CG B:GLN137 4.9 18.1 1.0 OD2 B:ASP154 4.9 27.7 1.0 O B:ASN12 5.0 24.1 1.0

B.Xue, C.Leyrat, J.M.Grimes, R.C.Robinson. Structural Basis of Thymosin-Beta 4/Profilin Exchange Leading to Actin Filament Polymerization. Proc.Natl.Acad.Sci.Usa V. 111 E4596 2014.
ISSN: ESSN 1091-6490
PubMed: 25313062
DOI: 10.1073/PNAS.1412271111