Calcium in PDB 4rib: FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna

All present enzymatic activity of FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna:
3.1.4.1;

The structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna, PDB code: 4rib was solved by N.P.Pavletich, R.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 3.25
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	97.697, 110.989, 105.427, 90.00, 103.75, 90.00
R / Rfree (%)	22.6 / 26.5

The binding sites of Calcium atom in the FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna (pdb code 4rib). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna, PDB code: 4rib:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1101 b:0.1 occ:1.00 OD2 A:ASP960 2.3 0.1 1.0 O A:VAL976 2.3 0.3 1.0 OE2 A:GLU975 2.3 0.0 1.0 OP1 U:DC4 2.4 0.9 1.0 CG A:ASP960 3.1 96.4 1.0 OD1 A:ASP960 3.4 97.9 1.0 OE2 A:GLU815 3.4 0.5 1.0 P U:DC4 3.5 0.2 1.0 C A:VAL976 3.5 0.6 1.0 CD A:GLU975 3.6 0.4 1.0 OP2 U:DC4 3.8 0.7 1.0 NZ A:LYS977 4.1 0.1 1.0 O5' U:DC4 4.1 0.5 1.0 OE1 A:GLU975 4.2 0.8 1.0 N A:VAL976 4.3 0.9 1.0 CA A:VAL976 4.3 0.6 1.0 CD A:LYS977 4.4 0.4 1.0 CB A:ASP960 4.5 92.5 1.0 CB A:VAL976 4.5 0.8 1.0 N A:LYS977 4.5 0.1 1.0 CD A:GLU815 4.6 0.5 1.0 CG A:GLU975 4.6 0.9 1.0 CE A:LYS977 4.7 0.6 1.0 CA A:LYS977 4.7 0.7 1.0 O3' U:DC3 4.8 0.3 1.0 C5' U:DC4 4.9 0.5 1.0 OD1 A:ASP981 5.0 0.9 1.0 NE2 A:GLN987 5.0 95.5 1.0

Calcium binding site 2 out of 2 in the FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of FAN1 Nuclease Bound to 5' Phosphorylated P(Dt) Single Flap Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1101 b:85.9 occ:1.00 O B:VAL976 2.3 89.1 1.0 OD2 B:ASP960 2.3 83.9 1.0 OE2 B:GLU975 2.3 84.3 1.0 CG B:ASP960 3.1 82.4 1.0 OD1 B:ASP960 3.3 84.5 1.0 OE2 B:GLU815 3.4 0.8 1.0 C B:VAL976 3.5 88.1 1.0 CD B:GLU975 3.6 83.4 1.0 NZ B:LYS977 4.2 89.9 1.0 N B:VAL976 4.2 84.8 1.0 OE1 B:GLU975 4.3 82.6 1.0 CA B:VAL976 4.3 85.8 1.0 CD B:LYS977 4.3 88.9 1.0 CB B:VAL976 4.4 85.2 1.0 CB B:ASP960 4.4 79.1 1.0 N B:LYS977 4.5 89.2 1.0 CD B:GLU815 4.6 0.0 1.0 CG B:GLU975 4.6 83.6 1.0 CA B:LYS977 4.7 89.8 1.0 CE B:LYS977 4.7 89.3 1.0 CG1 B:VAL976 4.9 84.3 1.0

R.Wang, N.Persky, B.Yoo, O.Ouerfelli, A.Smogorzewska, S.Elledge, N.P.Pavletich. Mechanism of Dna Interstrand Cross-Link Processing By Repair Nuclease FAN1 Science 2014.
ISSN: ESSN 1095-9203
DOI: 10.1126/SCIENCE.1258973