Calcium in PDB 4ric: FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna

All present enzymatic activity of FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna:
3.1.4.1;

The structure of FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna, PDB code: 4ric was solved by N.P.Pavletich, R.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	97.697, 110.989, 105.427, 90.00, 103.75, 90.00
R / Rfree (%)	22.3 / 25.7

The binding sites of Calcium atom in the FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna (pdb code 4ric). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna, PDB code: 4ric:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1101 b:88.6 occ:1.00 OP1 U:DC4 2.2 99.3 1.0 OD2 A:ASP960 2.3 85.4 1.0 OE2 A:GLU975 2.3 83.6 1.0 O A:VAL976 2.3 89.0 1.0 CG A:ASP960 3.0 82.5 1.0 OD1 A:ASP960 3.0 86.6 1.0 CD A:GLU975 3.5 82.0 1.0 P U:DC4 3.6 0.8 1.0 C A:VAL976 3.6 88.0 1.0 OP2 U:DC4 4.1 0.4 1.0 OE2 A:GLU815 4.1 97.6 1.0 OE1 A:GLU975 4.3 82.9 1.0 N A:VAL976 4.3 81.8 1.0 CA A:VAL976 4.4 84.7 1.0 O5' U:DC4 4.4 0.7 1.0 CB A:ASP960 4.4 76.4 1.0 CG A:GLU975 4.6 80.2 1.0 N A:LYS977 4.6 89.6 1.0 CD A:LYS977 4.6 88.6 1.0 CB A:VAL976 4.7 84.3 1.0 O3' U:DC3 4.7 0.9 1.0 NE2 A:GLN987 4.7 82.1 1.0 OD2 A:ASP981 4.8 0.7 1.0 CA A:LYS977 4.8 91.5 1.0 NZ A:LYS977 4.9 87.3 1.0 CE A:LYS977 5.0 88.0 1.0

Calcium binding site 2 out of 2 in the FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of FAN1 Nuclease Bound to 5' Hydroxyl (Dt-Dt) Single Flap Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1101 b:67.4 occ:1.00 OD2 B:ASP960 2.3 67.2 1.0 OE2 B:GLU975 2.3 63.2 1.0 O B:VAL976 2.3 68.6 1.0 CG B:ASP960 3.0 68.4 1.0 OD1 B:ASP960 3.0 73.8 1.0 CD B:GLU975 3.5 62.9 1.0 C B:VAL976 3.6 65.6 1.0 OE1 B:GLU975 4.3 64.6 1.0 OE2 B:GLU815 4.3 74.7 1.0 N B:VAL976 4.3 62.0 1.0 CA B:VAL976 4.3 63.1 1.0 CB B:ASP960 4.4 64.5 1.0 CD B:LYS977 4.5 73.6 1.0 CG B:GLU975 4.5 63.3 1.0 N B:LYS977 4.6 68.8 1.0 CB B:VAL976 4.6 62.5 1.0 NE2 B:GLN987 4.8 67.7 1.0 CA B:LYS977 4.8 72.6 1.0 OD2 B:ASP981 4.9 0.8 1.0 NZ B:LYS977 4.9 73.1 1.0 CE B:LYS977 5.0 73.8 1.0

N.P.Pavletich, R.Wang. Mechanism of Dna Interstrand Cross-Link Processing By Repair Nuclease FAN1 Science 2014.
ISSN: ESSN 1095-9203
DOI: 10.1126/SCIENCE.1258973