Calcium in PDB 4s10: Gelsolin Nanobody Shielding Mutant Plasma Gelsolin From Furin Proteolysis

The structure of Gelsolin Nanobody Shielding Mutant Plasma Gelsolin From Furin Proteolysis, PDB code: 4s10 was solved by J.Wongsantichon, R.C.Robinson, J.Gettemans, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.72 / 2.61
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	37.598, 46.334, 76.438, 103.00, 92.03, 89.85
R / Rfree (%)	22.3 / 31.2

The binding sites of Calcium atom in the Gelsolin Nanobody Shielding Mutant Plasma Gelsolin From Furin Proteolysis (pdb code 4s10). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Gelsolin Nanobody Shielding Mutant Plasma Gelsolin From Furin Proteolysis, PDB code: 4s10:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Gelsolin Nanobody Shielding Mutant Plasma Gelsolin From Furin Proteolysis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Gelsolin Nanobody Shielding Mutant Plasma Gelsolin From Furin Proteolysis within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca301 b:35.5 occ:1.00 OD2 C:ASP187 2.2 46.0 1.0 O C:GLY186 2.5 27.1 1.0 OD2 C:ASP259 2.5 29.6 1.0 OE2 C:GLU209 2.6 37.1 1.0 OD1 C:ASP259 2.8 25.7 1.0 OE1 C:GLU209 2.9 21.1 1.0 CG C:ASP259 3.0 24.5 1.0 CD C:GLU209 3.1 37.0 1.0 CG C:ASP187 3.4 35.8 1.0 C C:GLY186 3.5 24.4 1.0 ND2 C:ASN206 4.0 23.4 1.0 CA C:ASP187 4.1 20.0 1.0 N C:ASP187 4.2 20.6 1.0 OD1 C:ASP187 4.2 29.8 1.0 CB C:ASP187 4.3 20.3 1.0 CA C:GLY186 4.5 21.1 1.0 CB C:ASP259 4.5 28.4 1.0 CG C:GLU209 4.6 21.3 1.0 O C:ASN204 4.6 42.6 1.0 NZ C:LYS166 4.7 39.8 1.0 N C:ASN206 4.8 21.8 1.0 N C:GLY167 4.8 27.5 1.0 CG C:ASN206 4.8 23.1 1.0 O C:THR257 4.9 54.6 1.0 CG C:LYS166 4.9 25.5 1.0

Calcium binding site 2 out of 2 in the Gelsolin Nanobody Shielding Mutant Plasma Gelsolin From Furin Proteolysis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Gelsolin Nanobody Shielding Mutant Plasma Gelsolin From Furin Proteolysis within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca301 b:59.5 occ:1.00 OD2 D:ASP187 2.2 39.6 1.0 OD1 D:ASP259 2.3 25.5 1.0 O D:GLY186 2.5 23.6 1.0 OE2 D:GLU209 2.8 32.1 1.0 O D:HOH405 2.8 26.0 1.0 OD2 D:ASP259 2.9 38.4 1.0 CG D:ASP259 3.0 33.1 1.0 OE1 D:GLU209 3.0 22.6 1.0 CD D:GLU209 3.3 22.6 1.0 CG D:ASP187 3.5 32.4 1.0 C D:GLY186 3.5 22.7 1.0 ND2 D:ASN206 4.1 26.7 1.0 CA D:ASP187 4.1 25.6 1.0 N D:ASP187 4.2 21.9 1.0 OD1 D:ASP187 4.3 33.6 1.0 CB D:ASP187 4.4 31.4 1.0 NZ D:LYS166 4.4 27.5 1.0 CA D:GLY186 4.5 30.3 1.0 CB D:ASP259 4.5 31.4 1.0 O D:THR257 4.6 49.5 1.0 O D:ASN204 4.6 33.9 1.0 CG D:GLU209 4.7 22.1 1.0 CG D:ASN206 4.8 32.6 1.0 N D:ASN206 4.9 23.9 1.0 N D:GLY167 4.9 39.0 1.0 CG D:LYS166 5.0 35.6 1.0

W.Van Overbeke, J.Wongsantichon, I.Everaert, A.Verhelle, O.Zwaenepoel, A.Loonchanta, L.D.Burtnick, A.De Ganck, T.Hochepied, J.Haigh, C.Cuvelier, W.Derave, R.C.Robinson, J.Gettemans. An Er-Directed Gelsolin Nanobody Targets the First Step in Amyloid Formation in A Gelsolin Amyloidosis Mouse Model. Hum.Mol.Genet. V. 24 2492 2015.
ISSN: ISSN 0964-6906
PubMed: 25601851
DOI: 10.1093/HMG/DDV010