Calcium in PDB 4umo: Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin

The structure of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin, PDB code: 4umo was solved by D.Sachyani, J.A.Hirsch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.675 / 3.00
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	151.760, 151.760, 56.091, 90.00, 90.00, 120.00
R / Rfree (%)	21.33 / 24.66

The structure of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin also contains other interesting chemical elements:

Potassium

(K)

4 atoms

The binding sites of Calcium atom in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin (pdb code 4umo). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin, PDB code: 4umo:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca201 b:49.4 occ:0.37 OD1 C:ASP24 2.4 0.4 1.0 OE2 C:GLU31 2.4 0.3 1.0 OE1 C:GLU31 2.4 64.0 1.0 OD2 C:ASP22 2.4 0.8 1.0 OD1 C:ASP20 2.4 80.8 1.0 OD1 C:ASP22 2.4 0.5 1.0 OD2 C:ASP24 2.4 1.0 1.0 O C:THR26 2.4 95.0 1.0 CD C:GLU31 2.6 84.6 1.0 CG C:ASP24 2.7 1.0 1.0 CG C:ASP22 2.7 0.9 1.0 C C:THR26 3.5 98.2 1.0 CG C:ASP20 3.6 90.2 1.0 OG1 C:THR26 3.9 0.7 1.0 CG C:GLU31 4.0 82.7 1.0 CB C:ASP24 4.1 0.8 1.0 CB C:ASP22 4.2 0.7 1.0 OD2 C:ASP20 4.3 92.0 1.0 N C:ILE27 4.4 92.0 1.0 CG2 C:THR28 4.4 88.6 1.0 CA C:ILE27 4.4 82.1 1.0 CA C:THR26 4.4 98.6 1.0 N C:THR28 4.4 82.0 1.0 N C:THR26 4.5 98.6 1.0 CB C:ASP20 4.6 68.7 1.0 CA C:ASP20 4.6 69.7 1.0 CB C:THR26 4.7 0.4 1.0 OG1 C:THR28 4.7 89.0 1.0 N C:ASP24 4.8 0.3 1.0 C C:ILE27 4.8 85.8 1.0 N C:ASP22 4.8 0.6 1.0 CA C:ASP24 5.0 0.8 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca202 b:46.7 occ:0.60 OE1 C:GLU67 2.4 84.8 1.0 OD1 C:ASP56 2.4 85.0 1.0 OD2 C:ASP58 2.4 0.1 1.0 OD1 C:ASP58 2.4 0.5 1.0 OE2 C:GLU67 2.4 73.0 1.0 O C:THR62 2.4 74.6 1.0 OD1 C:ASN60 2.4 0.9 1.0 CG C:ASP58 2.7 0.5 1.0 CD C:GLU67 2.7 74.8 1.0 CG C:ASN60 3.4 0.2 1.0 CG C:ASP56 3.5 87.9 1.0 C C:THR62 3.6 75.5 1.0 ND2 C:ASN60 3.9 99.8 1.0 OD2 C:ASP64 4.1 0.6 1.0 CB C:ASP58 4.2 0.5 1.0 CG C:GLU67 4.2 73.8 1.0 OD2 C:ASP56 4.2 82.8 1.0 OG1 C:THR62 4.3 0.2 1.0 N C:ASP64 4.3 74.3 1.0 CA C:ILE63 4.3 65.4 1.0 N C:ILE63 4.4 71.9 1.0 N C:ASP58 4.4 95.6 1.0 CG C:ASP64 4.4 94.0 1.0 CB C:ASP56 4.5 85.0 1.0 N C:THR62 4.5 94.1 1.0 CA C:THR62 4.5 79.7 1.0 CA C:ASP56 4.6 77.8 1.0 CB C:ASN60 4.6 0.0 1.0 N C:ASN60 4.6 95.2 1.0 N C:ALA57 4.7 80.0 1.0 C C:ILE63 4.7 69.3 1.0 OD1 C:ASP64 4.8 92.7 1.0 CA C:ASP58 4.8 98.8 1.0 N C:GLY59 4.9 0.3 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca201 b:62.7 occ:0.63 OD1 D:ASP22 2.4 0.5 1.0 OD1 D:ASP20 2.4 75.7 1.0 OE2 D:GLU31 2.4 90.3 1.0 OE1 D:GLU31 2.4 83.4 1.0 OD2 D:ASP22 2.4 0.5 1.0 OD1 D:ASP24 2.4 95.4 1.0 O D:THR26 2.4 82.2 1.0 CD D:GLU31 2.6 86.4 1.0 CG D:ASP22 2.7 0.1 1.0 CG D:ASP24 3.2 96.3 1.0 OD2 D:ASP24 3.3 98.3 1.0 C D:THR26 3.5 80.8 1.0 CG D:ASP20 3.6 74.2 1.0 CG2 D:THR26 4.0 84.9 1.0 CG D:GLU31 4.1 80.8 1.0 CB D:ASP22 4.2 0.5 1.0 CA D:ILE27 4.3 84.8 1.0 N D:ILE27 4.4 78.3 1.0 CA D:ASP20 4.4 71.4 1.0 OD2 D:ASP20 4.4 75.7 1.0 N D:THR28 4.4 85.8 1.0 N D:THR26 4.5 89.0 1.0 CA D:THR26 4.5 86.4 1.0 CG2 D:THR28 4.5 90.6 1.0 CB D:ASP20 4.5 66.3 1.0 N D:ASP22 4.5 0.4 1.0 CB D:ASP24 4.6 95.2 1.0 OG1 D:THR28 4.7 94.7 1.0 C D:ASP20 4.8 78.7 1.0 N D:ASP24 4.8 97.2 1.0 C D:ILE27 4.8 89.6 1.0 N D:LYS21 4.9 89.8 1.0 CA D:ASP22 4.9 0.9 1.0 CB D:THR26 4.9 90.4 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca202 b:59.5 occ:0.63 OD1 D:ASP56 2.4 88.6 1.0 OE1 D:GLU67 2.4 64.6 1.0 OD2 D:ASP58 2.4 0.2 1.0 OD1 D:ASP58 2.4 0.8 1.0 OE2 D:GLU67 2.4 67.3 1.0 O D:THR62 2.4 89.7 1.0 OD1 D:ASN60 2.4 0.4 1.0 CG D:ASP58 2.7 0.9 1.0 CD D:GLU67 2.7 66.5 1.0 CG D:ASN60 3.4 94.9 1.0 CG D:ASP56 3.5 93.6 1.0 C D:THR62 3.6 86.6 1.0 ND2 D:ASN60 3.9 97.5 1.0 OD2 D:ASP64 4.1 0.1 1.0 CB D:ASP58 4.2 80.3 1.0 CG D:GLU67 4.2 68.2 1.0 OD2 D:ASP56 4.2 95.8 1.0 N D:ASP64 4.2 62.9 1.0 OG1 D:THR62 4.3 0.3 1.0 CA D:ILE63 4.3 64.9 1.0 N D:ILE63 4.4 76.4 1.0 CG D:ASP64 4.4 93.3 1.0 N D:ASP58 4.4 87.8 1.0 CB D:ASP56 4.5 89.0 1.0 N D:THR62 4.5 85.3 1.0 CA D:THR62 4.6 86.3 1.0 CA D:ASP56 4.6 79.2 1.0 CB D:ASN60 4.6 91.4 1.0 N D:ASN60 4.6 93.3 1.0 N D:ALA57 4.7 79.8 1.0 C D:ILE63 4.7 65.8 1.0 OD1 D:ASP64 4.8 96.6 1.0 CA D:ASP58 4.8 85.5 1.0 N D:GLY59 4.9 91.0 1.0 CB D:ASP64 5.0 80.5 1.0

D.Sachyani, M.Dvir, R.Strulovich, G.Tria, W.Tobelaim, A.Peretz, O.Pongs, D.Svergun, B.Attali, J.A.Hirsch. Structural Basis of A KV7.1 Potassium Channel Gating Module: Studies of the Intracellular C-Terminal Domain in Complex with Calmodulin Structure 2014.
ISSN: ESSN 1878-4186
DOI: 10.1016/J.STR.2014.07.016