Calcium in PDB 4uoj: Structure of Fungal Beta-Mannosidase (GH2) From Trichoderma Harzianum

The structure of Structure of Fungal Beta-Mannosidase (GH2) From Trichoderma Harzianum, PDB code: 4uoj was solved by J.R.C.Muniz, R.Aparicio, J.C.Santos, A.S.Nascimento, A.M.Golubev, I.Polikarpov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	117.23 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	165.161, 165.631, 123.562, 90.00, 90.00, 90.00
R / Rfree (%)	17.851 / 20.113

The structure of Structure of Fungal Beta-Mannosidase (GH2) From Trichoderma Harzianum also contains other interesting chemical elements:

Cadmium	(Cd)	28 atoms
Chlorine	(Cl)	1 atom
Sodium	(Na)	3 atoms

The binding sites of Calcium atom in the Structure of Fungal Beta-Mannosidase (GH2) From Trichoderma Harzianum (pdb code 4uoj). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Structure of Fungal Beta-Mannosidase (GH2) From Trichoderma Harzianum, PDB code: 4uoj:

Calcium binding site 1 out of 1 in the Structure of Fungal Beta-Mannosidase (GH2) From Trichoderma Harzianum


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Fungal Beta-Mannosidase (GH2) From Trichoderma Harzianum within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1996 b:54.9 occ:1.00 ND1 B:HIS848 2.5 46.5 1.0 O B:HOH2637 2.7 39.9 1.0 O B:HOH2682 2.8 33.1 1.0 O B:HOH2544 3.1 23.9 1.0 CE1 B:HIS848 3.3 47.2 1.0 CG B:HIS848 3.5 45.0 1.0 N B:HIS848 3.9 43.6 1.0 CG2 B:VAL905 3.9 46.7 1.0 CB B:HIS848 3.9 44.4 1.0 C B:ASN847 4.1 45.4 1.0 NH2 B:ARG735 4.1 36.6 1.0 CA B:ASN847 4.2 41.3 1.0 O B:HOH2543 4.2 44.8 1.0 O B:HOH2639 4.4 40.9 1.0 OD1 B:ASN847 4.4 45.2 1.0 NE2 B:HIS848 4.4 49.0 1.0 O B:ALA903 4.5 42.1 1.0 CA B:HIS848 4.5 44.2 1.0 N B:VAL905 4.5 42.5 1.0 CD2 B:HIS848 4.6 47.9 1.0 NZ B:LYS684 4.7 47.3 1.0 O B:ASN847 4.8 43.8 1.0 CA B:PHE904 4.8 40.2 1.0 CZ B:ARG735 4.8 44.1 1.0 CB B:VAL905 4.8 46.7 1.0 O B:HOH2579 4.8 37.2 1.0 N B:ASN847 5.0 41.4 1.0 C B:PHE904 5.0 43.0 1.0

A.S.Nascimento, J.R.C.Muniz, R.Aparicio, A.M.Golubev, I.Polikarpov. Insights Into the Structure and Function of Fungal Beta- Mannosidases From Glycoside Hydrolase Family 2 Based on Multiple Crystal Structures of the Trichoderma Harzianum Enzyme. Febs J. V. 281 4165 2014.
ISSN: ISSN 1742-464X
PubMed: 24975648
DOI: 10.1111/FEBS.12894