Calcium in PDB 4v0c: Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin

The structure of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin, PDB code: 4v0c was solved by D.Sachyani, J.A.Hirsch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.784 / 2.86
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	152.092, 152.092, 56.330, 90.00, 90.00, 120.00
R / Rfree (%)	22.59 / 26.79

The binding sites of Calcium atom in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin (pdb code 4v0c). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin, PDB code: 4v0c:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca201 b:0.1 occ:1.00 OD2 C:ASP22 2.3 0.5 1.0 OD1 C:ASP24 2.3 0.5 1.0 O C:THR26 2.4 0.1 1.0 OE2 C:GLU31 2.4 0.8 1.0 OG1 C:THR26 2.4 0.2 1.0 OD1 C:ASP20 2.4 0.4 1.0 OD2 C:ASP24 2.4 0.7 1.0 CG C:ASP24 2.7 0.8 1.0 C C:THR26 3.1 0.0 1.0 CG C:ASP22 3.2 0.6 1.0 CB C:ASP22 3.4 0.5 1.0 CD C:GLU31 3.4 0.8 1.0 CB C:THR26 3.5 1.0 1.0 CG C:ASP20 3.5 0.2 1.0 CA C:THR26 3.6 0.4 1.0 N C:THR26 3.6 0.4 1.0 OE1 C:GLU31 3.7 0.6 1.0 OD2 C:ASP20 4.0 0.6 1.0 N C:ILE27 4.1 99.2 1.0 CB C:ASP24 4.2 0.5 1.0 OD1 C:ASP22 4.3 0.4 1.0 CA C:ILE27 4.5 90.5 1.0 CG2 C:THR28 4.6 0.0 1.0 CG2 C:THR26 4.7 0.3 1.0 CA C:ASP22 4.7 0.5 1.0 CG C:GLU31 4.8 0.9 1.0 CB C:ASP20 4.8 90.1 1.0 N C:GLY25 4.8 0.2 1.0 N C:ASP24 4.8 0.1 1.0 N C:THR28 4.9 95.4 1.0 C C:GLY25 4.9 0.6 1.0 CA C:ASP24 5.0 0.8 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca202 b:0.5 occ:0.83 ND2 C:ASN60 2.3 0.1 1.0 OE1 C:GLU67 2.4 94.2 1.0 OD1 C:ASP58 2.4 0.5 1.0 OD1 C:ASP56 2.4 0.1 1.0 O C:THR62 2.4 0.5 1.0 OD1 C:ASN60 2.4 0.1 1.0 CG C:ASN60 2.6 0.4 1.0 CD C:GLU67 3.3 0.0 1.0 CG C:ASP58 3.4 0.9 1.0 C C:THR62 3.5 0.7 1.0 OE2 C:GLU67 3.6 0.6 1.0 CG C:ASP56 3.6 0.6 1.0 OD2 C:ASP58 3.7 0.7 1.0 N C:ASP64 4.1 93.1 1.0 CB C:ASN60 4.1 0.4 1.0 N C:ILE63 4.2 91.5 1.0 CA C:ILE63 4.2 90.6 1.0 OG1 C:THR62 4.2 0.3 1.0 OD2 C:ASP56 4.3 0.8 1.0 C C:ILE63 4.5 86.3 1.0 CA C:THR62 4.5 0.3 1.0 N C:THR62 4.5 0.8 1.0 CG C:GLU67 4.7 94.4 1.0 CB C:ASP56 4.7 0.4 1.0 CB C:ASP58 4.7 0.7 1.0 N C:ASN60 4.8 0.9 1.0 OD2 C:ASP64 4.8 0.6 1.0 CB C:ASP64 4.8 0.4 1.0 CG C:ASP64 4.9 0.0 1.0 CA C:ASN60 5.0 0.8 1.0 CB C:THR62 5.0 0.9 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca201 b:0.2 occ:1.00 OD2 D:ASP22 2.3 0.3 1.0 OD1 D:ASP20 2.4 0.7 1.0 OD1 D:ASP24 2.4 0.6 1.0 OE2 D:GLU31 2.4 0.8 1.0 O D:THR26 2.4 0.8 1.0 OD2 D:ASP24 2.4 0.3 1.0 OE1 D:GLU31 2.4 0.6 1.0 OG1 D:THR26 2.4 0.2 1.0 CG D:ASP22 2.6 0.3 1.0 CB D:ASP22 2.7 0.4 1.0 CD D:GLU31 2.7 0.8 1.0 CG D:ASP24 2.7 0.3 1.0 C D:THR26 3.3 0.1 1.0 OD1 D:ASP22 3.6 1.0 1.0 CG D:ASP20 3.6 0.9 1.0 CB D:THR26 3.6 0.2 1.0 CA D:THR26 3.9 0.1 1.0 N D:THR26 4.0 0.8 1.0 CA D:ASP22 4.1 0.7 1.0 CG D:GLU31 4.1 0.2 1.0 CB D:ASP24 4.2 0.3 1.0 OD2 D:ASP20 4.4 1.0 1.0 N D:ILE27 4.4 98.6 1.0 N D:ASP22 4.5 0.3 1.0 CB D:ASP20 4.6 0.5 1.0 CG2 D:THR26 4.7 0.7 1.0 CA D:ILE27 4.8 86.2 1.0 N D:ASP24 4.8 0.8 1.0 CA D:ASP20 4.8 0.5 1.0 C D:ASP22 4.9 1.0 1.0 CA D:ASP24 5.0 0.8 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of the KV7.1 Proximal C-Terminal Domain in Complex with Calmodulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca202 b:0.9 occ:1.00 OD1 D:ASP56 2.4 1.0 1.0 OD2 D:ASP58 2.4 0.6 1.0 OE1 D:GLU67 2.4 0.2 1.0 OE2 D:GLU67 2.4 94.3 1.0 O D:THR62 2.4 0.2 1.0 OD1 D:ASN60 2.4 0.5 1.0 CD D:GLU67 2.7 0.4 1.0 O D:HOH2001 3.1 0.7 1.0 CG D:ASP58 3.2 0.7 1.0 CG D:ASP56 3.4 0.2 1.0 CG D:ASN60 3.4 1.0 1.0 C D:THR62 3.5 0.7 1.0 OD1 D:ASP58 3.5 0.2 1.0 ND2 D:ASN60 4.0 0.8 1.0 OD2 D:ASP56 4.1 0.3 1.0 CG D:GLU67 4.2 93.5 1.0 CA D:ILE63 4.2 85.2 1.0 CB D:ASP56 4.3 0.9 1.0 N D:ILE63 4.3 92.1 1.0 N D:ASP64 4.3 80.6 1.0 CB D:ASP58 4.5 0.1 1.0 N D:THR62 4.6 0.2 1.0 CA D:ASP56 4.6 0.1 1.0 N D:ASP58 4.6 0.4 1.0 CA D:THR62 4.6 0.3 1.0 C D:ILE63 4.6 88.2 1.0 OD2 D:ASP64 4.6 0.4 1.0 CB D:ASN60 4.6 0.8 1.0 OG1 D:THR62 4.7 0.6 1.0 CG D:ASP64 4.7 97.3 1.0 N D:ASN60 4.7 0.4 1.0 OD1 D:ASP64 4.7 0.9 1.0 N D:ALA57 4.8 1.0 1.0 N D:GLY59 5.0 0.0 1.0 CA D:ASP58 5.0 0.2 1.0

D.Sachyani, M.Dvir, R.Strulovich, G.Tria, W.Tobelaim, A.Peretz, O.Pongs, D.Svergun, B.Attali, J.A.Hirsch. Structural Basis of A KV7.1 Potassium Channel Gating Module: Studies of the Intracellular C-Terminal Domain in Complex with Calmodulin Structure 2014.
ISSN: ESSN 1878-4186
DOI: 10.1016/J.STR.2014.07.016