Calcium in PDB 4wo1: Crystal Structure of the DAP12 Transmembrane Domain in Lipid Cubic Phase

The structure of Crystal Structure of the DAP12 Transmembrane Domain in Lipid Cubic Phase, PDB code: 4wo1 was solved by M.J.Call, M.E.Call, K.Knoblich, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.12 / 2.14
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	50.797, 43.685, 50.605, 90.00, 90.00, 90.00
R / Rfree (%)	23.2 / 29.6

The binding sites of Calcium atom in the Crystal Structure of the DAP12 Transmembrane Domain in Lipid Cubic Phase (pdb code 4wo1). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the DAP12 Transmembrane Domain in Lipid Cubic Phase, PDB code: 4wo1:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of the DAP12 Transmembrane Domain in Lipid Cubic Phase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the DAP12 Transmembrane Domain in Lipid Cubic Phase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca101 b:26.5 occ:0.50 CA A:CA101 0.0 26.5 0.5 O A:HOH203 1.5 21.3 0.5 OD2 B:ASP23 2.2 31.1 1.0 O D:HOH205 2.3 37.4 1.0 O A:HOH205 2.3 27.7 1.0 CA A:CA101 2.4 42.2 0.5 O A:HOH203 2.4 41.2 0.5 OD2 A:ASP23 2.5 30.9 1.0 OD1 A:ASP23 2.7 44.5 1.0 CG A:ASP23 2.9 32.0 1.0 O B:HOH204 3.0 43.6 1.0 CG B:ASP23 3.3 34.2 1.0 OD1 B:ASP23 3.8 36.8 1.0 O A:HOH204 3.9 25.6 0.5 OD1 D:ASP23 4.0 29.2 1.0 O C:HOH205 4.2 30.0 1.0 OG1 D:THR27 4.3 23.4 1.0 OG1 A:THR27 4.3 24.4 1.0 CB A:ASP23 4.4 23.5 1.0 O C:HOH204 4.5 28.5 0.7 OG1 B:THR27 4.5 28.7 1.0 CB B:ASP23 4.5 23.8 1.0 O B:ASP23 4.7 25.5 1.0 CA B:ASP23 4.8 18.3 1.0 CG2 D:THR27 4.9 18.3 1.0 CG D:ASP23 4.9 34.4 1.0 OD2 D:ASP23 4.9 36.5 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of the DAP12 Transmembrane Domain in Lipid Cubic Phase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the DAP12 Transmembrane Domain in Lipid Cubic Phase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca101 b:42.2 occ:0.50 CA A:CA101 0.0 42.2 0.5 O A:HOH203 0.1 41.2 0.5 OD1 A:ASP23 2.3 44.5 1.0 O A:HOH203 2.3 21.3 0.5 O C:HOH205 2.4 30.0 1.0 O C:HOH204 2.4 28.5 0.7 CA A:CA101 2.4 26.5 0.5 OD1 B:ASP23 2.6 36.8 1.0 O A:HOH204 2.8 25.6 0.5 OD2 B:ASP23 2.8 31.1 1.0 CG B:ASP23 3.1 34.2 1.0 CG A:ASP23 3.4 32.0 1.0 O A:HOH205 3.8 27.7 1.0 OD2 A:ASP23 3.8 30.9 1.0 O B:HOH204 3.9 43.6 1.0 OG1 B:THR27 4.0 28.7 1.0 OD1 C:ASP23 4.1 40.6 1.0 OG1 C:THR27 4.1 22.6 1.0 CG2 C:THR27 4.4 18.6 1.0 CB B:ASP23 4.6 23.8 1.0 O D:HOH205 4.6 37.4 1.0 O C:HOH210 4.7 59.0 1.0 CB A:ASP23 4.7 23.5 1.0 OG1 A:THR27 4.7 24.4 1.0 O A:ASP23 4.9 21.2 1.0 O B:ASP23 4.9 25.5 1.0 CB C:THR27 4.9 17.8 1.0 OD2 C:ASP23 4.9 34.2 1.0 CG C:ASP23 5.0 37.9 1.0

K.Knoblich, S.Park, M.Lutfi, L.Van 't Hag, C.E.Conn, S.A.Seabrook, J.Newman, P.E.Czabotar, W.Im, M.E.Call, M.J.Call. Transmembrane Complexes of DAP12 Crystallized in Lipid Membranes Provide Insights Into Control of Oligomerization in Immunoreceptor Assembly. Cell Rep V. 11 1184 2015.
ISSN: ESSN 2211-1247
PubMed: 25981043
DOI: 10.1016/J.CELREP.2015.04.045