Calcium in PDB 4wzf: Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Pseudo Beta-1, 3-Glucanase of Mycobacterium Tuberculosis

The structure of Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Pseudo Beta-1, 3-Glucanase of Mycobacterium Tuberculosis, PDB code: 4wzf was solved by W.Y.Dong, Z.F.Fu, G.Q.Peng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.78 / 1.70
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	135.133, 65.847, 67.449, 90.00, 105.17, 90.00
R / Rfree (%)	18.2 / 21.1

The binding sites of Calcium atom in the Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Pseudo Beta-1, 3-Glucanase of Mycobacterium Tuberculosis (pdb code 4wzf). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Pseudo Beta-1, 3-Glucanase of Mycobacterium Tuberculosis, PDB code: 4wzf:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Pseudo Beta-1, 3-Glucanase of Mycobacterium Tuberculosis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Pseudo Beta-1, 3-Glucanase of Mycobacterium Tuberculosis within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:19.6 occ:1.00 OD1 A:ASP289 2.2 18.3 1.0 O A:SER112 2.3 17.2 1.0 OG A:SER112 2.3 20.6 1.0 O A:ASP289 2.3 14.7 1.0 O A:GLU61 2.4 17.2 1.0 O A:HOH531 2.6 26.5 1.0 CB A:SER112 3.2 20.8 1.0 C A:ASP289 3.3 16.5 1.0 C A:SER112 3.4 18.6 1.0 CG A:ASP289 3.4 21.0 1.0 C A:GLU61 3.5 16.2 1.0 CA A:SER112 3.8 17.3 1.0 CA A:ASP289 3.8 15.8 1.0 CA A:GLU61 4.1 17.0 1.0 CB A:ASP289 4.2 15.4 1.0 OD2 A:ASP289 4.3 21.1 1.0 CB A:GLU61 4.4 17.9 1.0 O A:HOH512 4.4 36.4 1.0 N A:TRP290 4.5 14.7 1.0 N A:ASN113 4.5 17.6 1.0 OE1 A:GLU61 4.6 20.9 1.0 N A:PHE62 4.6 16.5 1.0 OD1 A:ASP63 4.7 25.6 1.0 CB A:PHE62 4.7 18.5 1.0 CB A:TRP290 4.7 16.9 1.0 CD1 A:TRP290 4.8 17.5 1.0 OD1 A:ASN113 4.8 19.7 1.0 O A:HOH690 4.9 28.6 1.0 C A:PHE62 4.9 18.3 1.0 CA A:TRP290 4.9 15.8 1.0 CA A:PHE62 5.0 17.5 1.0 CA A:ASN113 5.0 16.3 1.0 O A:PHE62 5.0 18.5 1.0

Calcium binding site 2 out of 2 in the Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Pseudo Beta-1, 3-Glucanase of Mycobacterium Tuberculosis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Pseudo Beta-1, 3-Glucanase of Mycobacterium Tuberculosis within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca401 b:17.9 occ:1.00 OD1 B:ASP289 2.3 15.7 1.0 OG B:SER112 2.3 18.4 1.0 O B:SER112 2.3 16.5 1.0 O B:HOH507 2.3 18.4 1.0 O B:ASP289 2.4 14.3 1.0 O B:GLU61 2.4 15.5 1.0 CB B:SER112 3.1 18.8 1.0 C B:SER112 3.3 17.8 1.0 C B:ASP289 3.4 15.0 1.0 CG B:ASP289 3.4 16.5 1.0 C B:GLU61 3.5 15.1 1.0 CA B:SER112 3.8 16.1 1.0 CA B:ASP289 3.9 14.5 1.0 CA B:GLU61 4.1 16.0 1.0 CB B:ASP289 4.3 14.3 1.0 OD2 B:ASP289 4.3 19.9 1.0 CB B:GLU61 4.4 16.8 1.0 N B:TRP290 4.5 13.8 1.0 N B:ASN113 4.5 15.0 1.0 OE1 B:GLU61 4.6 22.2 1.0 OD1 B:ASP63 4.6 24.5 1.0 N B:PHE62 4.6 16.0 1.0 CB B:PHE62 4.7 14.0 1.0 CB B:TRP290 4.7 14.2 1.0 CD1 B:TRP290 4.8 15.8 1.0 C B:PHE62 4.8 17.5 1.0 OD1 B:ASN113 4.9 19.9 1.0 CA B:TRP290 4.9 14.1 1.0 CA B:PHE62 4.9 15.8 1.0 O B:PHE62 5.0 15.9 1.0 CA B:ASN113 5.0 15.6 1.0

W.Dong, J.Huang, Y.Li, Y.Tan, Z.Shen, Y.Song, D.Wang, S.Xiao, H.Chen, Z.F.Fu, G.Peng. Crystal Structural Basis For RV0315, An Immunostimulatory Antigen and Inactive Beta-1,3-Glucanase of Mycobacterium Tuberculosis. Sci Rep V. 5 15073 2015.
ISSN: ESSN 2045-2322
PubMed: 26469317
DOI: 10.1038/SREP15073