Calcium in PDB 4z6a: Crystal Structure of A Fviia-Trypsin Chimera (Yt) in Complex with Soluble Tissue Factor

All present enzymatic activity of Crystal Structure of A Fviia-Trypsin Chimera (Yt) in Complex with Soluble Tissue Factor:
3.4.21.21;

The structure of Crystal Structure of A Fviia-Trypsin Chimera (Yt) in Complex with Soluble Tissue Factor, PDB code: 4z6a was solved by A.B.Sorensen, L.A.Svensson, P.S.Gandhi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.51 / 2.25
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	67.030, 81.700, 124.560, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 24.1

The binding sites of Calcium atom in the Crystal Structure of A Fviia-Trypsin Chimera (Yt) in Complex with Soluble Tissue Factor (pdb code 4z6a). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of A Fviia-Trypsin Chimera (Yt) in Complex with Soluble Tissue Factor, PDB code: 4z6a:

Calcium binding site 1 out of 1 in the Crystal Structure of A Fviia-Trypsin Chimera (Yt) in Complex with Soluble Tissue Factor


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of A Fviia-Trypsin Chimera (Yt) in Complex with Soluble Tissue Factor within 5.0Å range: probe atom residue distance (Å) B Occ H:Ca502 b:64.2 occ:1.00 OE1 H:GLU70 2.2 36.6 1.0 O H:GLU75 2.4 55.2 1.0 O H:ASP72 2.5 38.0 1.0 O H:HOH606 2.5 27.0 1.0 OE1 H:GLU80 2.5 33.6 1.0 O H:HOH687 2.5 34.2 1.0 OE2 H:GLU70 2.7 30.4 1.0 CD H:GLU70 2.8 27.6 1.0 HA H:HIS76 3.2 76.7 1.0 HG2 H:GLU80 3.4 37.0 1.0 C H:GLU75 3.5 53.7 1.0 H H:ASP72 3.5 37.9 1.0 C H:ASP72 3.5 36.3 1.0 CD H:GLU80 3.5 34.6 1.0 H H:HIS71 3.5 31.9 1.0 HA H:LEU73 3.8 45.4 1.0 CG H:GLU80 3.8 30.8 1.0 H H:GLU75 3.9 64.1 1.0 HG3 H:GLU80 3.9 37.0 1.0 O H:HOH669 4.0 33.6 1.0 N H:ASP72 4.0 31.6 1.0 CA H:HIS76 4.1 63.9 1.0 H H:ASP77 4.1 51.9 1.0 HA H:GLU70 4.1 33.1 1.0 N H:HIS76 4.2 64.3 1.0 HB3 H:ASP72 4.2 49.6 1.0 CG H:GLU70 4.3 28.4 1.0 CA H:ASP72 4.3 31.0 1.0 N H:LEU73 4.4 37.3 1.0 N H:HIS71 4.4 26.6 1.0 CA H:LEU73 4.5 37.8 1.0 CA H:GLU75 4.5 56.6 1.0 N H:GLU75 4.5 53.4 1.0 HG3 H:GLU70 4.5 34.0 1.0 OE2 H:GLU80 4.6 35.6 1.0 HD23 H:LEU73 4.7 41.2 1.0 HB2 H:GLU75 4.7 73.8 1.0 HG2 H:GLU70 4.8 34.0 1.0 CB H:ASP72 4.8 41.4 1.0 N H:ASP77 4.8 43.2 1.0 C H:LEU73 4.8 41.0 1.0 CA H:GLU70 4.9 27.6 1.0 C H:HIS71 4.9 31.0 1.0 HB2 H:HIS76 4.9 82.6 1.0 HB3 H:GLU75 5.0 73.8 1.0 H H:HIS76 5.0 77.1 1.0

A.B.Sorensen, J.J.Madsen, L.A.Svensson, A.A.Pedersen, H.Stergaard, M.T.Overgaard, O.H.Olsen, P.S.Gandhi. Molecular Basis of Enhanced Activity in Factor Viia-Trypsin Variants Conveys Insights Into Tissue Factor-Mediated Allosteric Regulation of Factor Viia Activity. J.Biol.Chem. V. 291 4671 2016.
ISSN: ESSN 1083-351X
PubMed: 26694616
DOI: 10.1074/JBC.M115.698613