Calcium in PDB 4z94: Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2

The structure of Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2, PDB code: 4z94 was solved by G.Rebowski, M.Boczkowska, R.Dominguez, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.81 / 2.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	71.234, 70.799, 81.324, 90.00, 101.74, 90.00
R / Rfree (%)	18.5 / 24.3

The binding sites of Calcium atom in the Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2 (pdb code 4z94). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2, PDB code: 4z94:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:26.2 occ:1.00 O1B A:ATP401 2.4 15.1 1.0 O A:HOH551 2.4 19.2 1.0 O3G A:ATP401 2.4 6.9 1.0 O A:HOH579 2.6 17.1 1.0 HZ1 A:LYS18 3.1 21.6 1.0 PG A:ATP401 3.5 28.7 1.0 HA3 A:GLY13 3.6 21.4 1.0 PB A:ATP401 3.7 11.9 1.0 O2G A:ATP401 3.7 19.6 1.0 HA2 A:GLY13 3.8 21.4 1.0 O3B A:ATP401 3.9 16.0 1.0 NZ A:LYS18 4.0 18.0 1.0 CA A:GLY13 4.2 17.9 1.0 HZ2 A:LYS18 4.2 21.6 1.0 HG21 A:VAL339 4.2 16.1 1.0 HZ3 A:LYS18 4.3 21.6 1.0 OE1 A:GLN137 4.3 11.2 1.0 O3A A:ATP401 4.4 17.5 1.0 CD A:GLN137 4.4 5.1 1.0 O A:HOH552 4.4 21.3 1.0 HG2 A:GLN137 4.4 18.0 1.0 O1A A:ATP401 4.4 6.5 1.0 O A:HOH513 4.5 18.2 1.0 OD2 A:ASP11 4.6 8.0 1.0 NE2 A:GLN137 4.6 11.3 1.0 HA3 A:GLY156 4.6 24.6 1.0 H A:SER14 4.6 18.9 1.0 HE22 A:GLN137 4.7 13.5 1.0 OD1 A:ASP11 4.7 10.7 1.0 HB3 A:GLN137 4.8 9.6 1.0 PA A:ATP401 4.8 20.4 1.0 O1G A:ATP401 4.8 10.6 1.0 OD2 A:ASP154 4.9 10.3 1.0 CG A:GLN137 4.9 15.0 1.0 O2B A:ATP401 4.9 20.5 1.0 HG11 A:VAL339 4.9 28.5 1.0 N A:GLY13 4.9 23.0 1.0 HE3 A:LYS18 5.0 22.1 1.0 HE21 A:GLN137 5.0 13.5 1.0

Calcium binding site 2 out of 3 in the Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:46.4 occ:1.00 O G:GLY90 2.4 36.6 1.0 O G:ALA92 2.5 34.9 1.0 OD2 G:ASP85 2.5 12.4 1.0 OE1 A:GLU167 2.6 32.4 1.0 C G:GLY90 3.2 37.5 1.0 OD1 G:ASP85 3.3 25.1 1.0 CG G:ASP85 3.3 31.2 1.0 HA2 G:GLY90 3.3 39.0 1.0 C G:ALA92 3.6 33.7 1.0 H G:ALA92 3.7 21.6 1.0 CD A:GLU167 3.7 33.2 1.0 CA G:GLY90 3.7 32.5 1.0 N G:ALA92 3.9 18.0 1.0 HA3 G:GLY90 4.0 39.0 1.0 N G:ARG91 4.1 24.1 1.0 HE21 G:GLN94 4.2 31.1 1.0 OE2 A:GLU167 4.2 31.1 1.0 HA G:VAL93 4.2 17.5 1.0 C G:ARG91 4.3 35.1 1.0 CA G:ALA92 4.3 25.0 1.0 HA G:ARG91 4.5 38.5 1.0 O A:HOH535 4.5 33.5 1.0 HB3 A:GLU167 4.5 38.6 1.0 CA G:ARG91 4.5 32.1 1.0 HB3 G:ALA92 4.6 20.4 1.0 N G:VAL93 4.6 25.5 1.0 HG3 G:GLN94 4.7 14.5 1.0 H G:ARG91 4.8 28.9 1.0 O A:HOH540 4.8 23.0 1.0 CB G:ASP85 4.8 17.5 1.0 NE2 G:GLN94 4.8 25.9 1.0 HB2 A:GLU167 4.9 38.6 1.0 CA G:VAL93 4.9 14.6 1.0 CG A:GLU167 4.9 33.6 1.0 HE2 A:TYR166 5.0 29.7 1.0 CB A:GLU167 5.0 32.1 1.0

Calcium binding site 3 out of 3 in the Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Actin Complex with A Chimera of Tropomodulin-1 and Leiomodin-1 Actin- Binding Site 2 within 5.0Å range: probe atom residue distance (Å) B Occ G:Ca2000 b:31.9 occ:1.00 O G:GLY41 2.2 18.6 1.0 O G:HOH2121 2.2 17.3 1.0 O G:HOH2122 2.3 31.5 1.0 O G:VAL121 2.4 28.4 1.0 OD1 G:ASP42 2.4 32.7 1.0 OE2 G:GLU73 2.4 26.8 1.0 OE1 G:GLU73 2.5 29.6 1.0 CD G:GLU73 2.8 27.6 1.0 HA G:ASP42 3.1 25.9 1.0 C G:GLY41 3.3 31.2 1.0 CG G:ASP42 3.5 30.4 1.0 C G:VAL121 3.6 27.6 1.0 HG13 G:VAL121 3.7 49.4 1.0 H G:SER70 3.8 16.7 1.0 CA G:ASP42 3.9 21.6 1.0 HA G:CYS69 3.9 39.3 1.0 N G:ASP42 4.0 25.9 1.0 HA3 G:GLY120 4.0 33.1 1.0 HA G:ALA122 4.2 31.5 1.0 N G:VAL121 4.2 32.3 1.0 O G:HOH2123 4.3 31.1 1.0 CG G:GLU73 4.3 26.1 1.0 HA3 G:GLY41 4.3 24.6 1.0 CB G:ASP42 4.3 18.1 1.0 C G:GLY120 4.3 19.2 1.0 H G:VAL121 4.4 38.8 1.0 HB2 G:CYS69 4.4 41.3 1.0 CA G:GLY41 4.4 20.5 1.0 OD2 G:ASP42 4.4 27.7 1.0 OG G:SER70 4.5 26.1 1.0 CA G:VAL121 4.5 24.9 1.0 CG1 G:VAL121 4.5 41.1 1.0 N G:ALA122 4.5 18.4 1.0 HG12 G:VAL121 4.6 49.4 1.0 N G:SER70 4.6 13.9 1.0 HG3 G:GLU73 4.6 31.3 1.0 C G:ALA122 4.6 40.5 1.0 CA G:ALA122 4.6 26.2 1.0 O G:GLY120 4.7 32.4 1.0 HG2 G:GLU73 4.7 31.3 1.0 CA G:GLY120 4.7 27.6 1.0 N G:SER123 4.7 36.2 1.0 HA G:SER123 4.8 39.8 1.0 CA G:CYS69 4.8 32.8 1.0 HA2 G:GLY41 4.8 24.6 1.0 H G:SER123 4.8 43.4 1.0 H G:ASP42 4.8 31.0 1.0 HB3 G:ASP42 4.8 21.8 1.0 HG G:SER70 4.9 31.3 1.0 HB2 G:GLU73 4.9 22.9 1.0

M.Boczkowska, G.Rebowski, E.Kremneva, P.Lappalainen, R.Dominguez. How Leiomodin and Tropomodulin Use A Common Fold For Different Actin Assembly Functions. Nat Commun V. 6 8314 2015.
ISSN: ESSN 2041-1723
PubMed: 26370058
DOI: 10.1038/NCOMMS9314