Calcium in PDB 4zfg: Dual-Specificity Fab 5A12 in Complex with Angiopoietin 2

The structure of Dual-Specificity Fab 5A12 in Complex with Angiopoietin 2, PDB code: 4zfg was solved by S.F.Harris, P.Wu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.21 / 2.27
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	181.668, 109.292, 43.946, 90.00, 100.47, 90.00
R / Rfree (%)	17.7 / 22.3

The binding sites of Calcium atom in the Dual-Specificity Fab 5A12 in Complex with Angiopoietin 2 (pdb code 4zfg). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Dual-Specificity Fab 5A12 in Complex with Angiopoietin 2, PDB code: 4zfg:

Calcium binding site 1 out of 1 in the Dual-Specificity Fab 5A12 in Complex with Angiopoietin 2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Dual-Specificity Fab 5A12 in Complex with Angiopoietin 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:54.4 occ:1.00 O A:CYS433 2.2 45.1 1.0 OD1 A:ASP431 2.3 48.6 1.0 OD2 A:ASP429 2.4 64.7 1.0 OD1 A:ASP429 2.4 59.9 1.0 O A:CYS435 2.4 45.5 1.0 O A:HOH707 2.6 49.3 1.0 O A:HOH730 2.7 56.0 1.0 CG A:ASP429 2.7 59.2 1.0 CG A:ASP431 3.2 51.0 1.0 C A:CYS433 3.3 46.0 1.0 OD2 A:ASP431 3.6 51.6 1.0 C A:CYS435 3.6 46.2 1.0 O A:ILE434 4.1 47.4 1.0 C A:ILE434 4.1 46.3 1.0 CA A:CYS433 4.1 43.2 1.0 N A:CYS433 4.1 45.0 1.0 CB A:ASP429 4.2 53.7 1.0 O A:ASP431 4.3 48.4 1.0 O H:HOH415 4.3 70.2 1.0 N A:ILE434 4.3 43.1 1.0 N A:CYS435 4.3 40.4 1.0 CB A:CYS433 4.3 43.5 1.0 CA A:LYS436 4.4 43.7 1.0 N A:LYS436 4.4 43.5 1.0 CA A:ILE434 4.5 42.4 1.0 CB A:ASP431 4.5 48.4 1.0 C A:ASP431 4.5 49.9 1.0 O A:HOH711 4.6 61.2 1.0 CA A:CYS435 4.6 42.0 1.0 O A:HOH736 4.7 67.5 1.0 O A:HOH819 4.7 85.4 1.0 N A:ASP431 4.7 49.3 1.0 CA A:ASP431 4.8 47.9 1.0

P.Koenig, C.V.Lee, S.Sanowar, P.Wu, J.Stinson, S.F.Harris, G.Fuh. Deep Sequencing-Guided Design of A High Affinity Dual Specificity Antibody to Target Two Angiogenic Factors in Neovascular Age-Related Macular Degeneration. J.Biol.Chem. V. 290 21773 2015.
ISSN: ESSN 1083-351X
PubMed: 26088137
DOI: 10.1074/JBC.M115.662783