Calcium in PDB 4zsf: Crystal Structure of Pre-Specific Restriction Endonuclease Bsawi-Dna Complex

The structure of Crystal Structure of Pre-Specific Restriction Endonuclease Bsawi-Dna Complex, PDB code: 4zsf was solved by G.Tamulaitiene, M.Rutkauskas, S.Grazulis, V.Siksnys, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.40 / 1.80
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	89.562, 136.336, 73.482, 90.00, 90.00, 90.00
R / Rfree (%)	15.2 / 18.8

The binding sites of Calcium atom in the Crystal Structure of Pre-Specific Restriction Endonuclease Bsawi-Dna Complex (pdb code 4zsf). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Pre-Specific Restriction Endonuclease Bsawi-Dna Complex, PDB code: 4zsf:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Pre-Specific Restriction Endonuclease Bsawi-Dna Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Pre-Specific Restriction Endonuclease Bsawi-Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca303 b:40.2 occ:0.47 O A:HOH477 2.4 36.7 1.0 O A:HOH444 2.4 26.5 1.0 O A:HOH521 2.4 36.2 1.0 O A:HOH609 2.5 42.0 1.0 O A:HOH502 2.5 36.9 1.0 O A:HOH516 2.6 41.0 1.0 OD2 A:ASP233 4.1 28.0 1.0 O A:HOH600 4.1 53.9 1.0 O A:ASN209 4.1 28.2 1.0 NE A:ARG234 4.2 23.6 1.0 NH2 A:ARG234 4.3 20.4 1.0 OD1 A:ASN218 4.7 24.6 1.0 O A:HOH436 4.7 38.2 1.0 CZ A:ARG234 4.8 20.4 1.0 OD1 A:ASP233 4.8 26.7 1.0 CG A:ASP233 4.9 26.2 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Pre-Specific Restriction Endonuclease Bsawi-Dna Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Pre-Specific Restriction Endonuclease Bsawi-Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca304 b:41.3 occ:1.00 OD2 A:ASP239 2.3 32.3 1.0 O A:HOH443 2.5 38.7 1.0 O A:HOH575 2.7 47.3 1.0 CG A:ASP239 3.2 28.5 1.0 OD1 A:ASP239 3.7 25.6 1.0 OE2 A:GLU243 4.2 27.2 1.0 O A:HOH588 4.3 48.0 1.0 CB A:ASP239 4.3 25.0 1.0 O A:HOH545 4.4 45.8 1.0 ND2 A:ASN241 4.7 30.2 1.0 O A:HOH451 4.9 21.9 1.0

G.Tamulaitis, M.Rutkauskas, M.Zaremba, S.Grazulis, G.Tamulaitiene, V.Siksnys. Functional Significance of Protein Assemblies Predicted By the Crystal Structure of the Restriction Endonuclease Bsawi. Nucleic Acids Res. V. 43 8100 2015.
ISSN: ESSN 1362-4962
PubMed: 26240380
DOI: 10.1093/NAR/GKV768