Calcium in PDB 5avj: Crystal Structure of Proteinase K From Engyodontium Album

All present enzymatic activity of Crystal Structure of Proteinase K From Engyodontium Album:
3.4.21.64;

The structure of Crystal Structure of Proteinase K From Engyodontium Album, PDB code: 5avj was solved by M.Sugahara, K.Numata, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.54 / 1.45
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	67.549, 67.549, 107.083, 90.00, 90.00, 90.00
R / Rfree (%)	16.3 / 18.4

The binding sites of Calcium atom in the Crystal Structure of Proteinase K From Engyodontium Album (pdb code 5avj). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Proteinase K From Engyodontium Album, PDB code: 5avj:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Proteinase K From Engyodontium Album


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Proteinase K From Engyodontium Album within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1006 b:4.1 occ:0.76 O A:VAL177 2.4 8.7 1.0 O A:PRO175 2.4 8.8 1.0 O A:HOH1202 2.4 8.9 1.0 O A:HOH1279 2.4 11.5 1.0 OD2 A:ASP200 2.4 8.7 1.0 O A:HOH1165 2.5 10.3 1.0 O A:HOH1342 2.5 13.1 1.0 OD1 A:ASP200 2.6 10.0 1.0 CG A:ASP200 2.9 8.9 1.0 C A:PRO175 3.5 7.0 1.0 C A:VAL177 3.6 7.4 1.0 O A:HOH1105 3.6 22.3 1.0 N A:VAL177 4.1 9.0 1.0 O A:VAL198 4.2 11.2 1.0 CA A:PRO175 4.3 9.0 1.0 CB A:ASP200 4.4 8.5 1.0 C A:SER176 4.4 9.1 1.0 N A:SER176 4.5 6.8 1.0 O A:HOH1431 4.5 18.2 1.0 CA A:CYS178 4.5 8.2 1.0 N A:CYS178 4.5 6.7 1.0 O A:GLU174 4.5 8.1 1.0 CA A:VAL177 4.6 7.8 1.0 N A:THR179 4.6 6.7 1.0 O A:HOH1264 4.7 11.6 1.0 O A:HOH1401 4.7 24.5 1.0 CA A:SER176 4.7 7.9 1.0 O A:HOH1447 4.8 29.1 1.0 OG1 A:THR179 4.9 7.9 1.0 SG A:CYS249 4.9 11.0 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Proteinase K From Engyodontium Album


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Proteinase K From Engyodontium Album within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1007 b:14.5 occ:0.78 O A:HOH1289 2.4 24.5 1.0 O A:THR16 2.4 16.0 1.0 OD2 A:ASP260 2.4 17.4 1.0 O A:HOH1375 2.5 19.8 1.0 O A:HOH1352 2.5 24.6 1.0 OD1 A:ASP260 2.5 19.8 1.0 O A:HOH1265 2.5 18.1 1.0 CG A:ASP260 2.7 18.8 1.0 C A:THR16 3.6 13.3 1.0 O A:HOH1113 4.1 24.0 1.0 CB A:ASP260 4.2 17.0 1.0 CA A:THR16 4.5 11.8 1.0 N A:SER17 4.5 11.7 1.0 CB A:THR16 4.5 13.1 1.0 OD1 A:ASN257 4.5 15.0 1.0 CA A:SER17 4.5 13.4 1.0 NH2 A:ARG12 4.6 12.1 1.0 O A:HOH1464 4.8 26.3 1.0 CG A:ASN257 4.8 13.3 1.0 O A:HOH1130 5.0 17.4 1.0 OG1 A:THR16 5.0 16.6 1.0 CB A:ASN257 5.0 10.7 1.0

K.Yazawa, M.Sugahara, K.Yutani, M.Takehira, K.Numata. Derivatization of Proteinase K with Heavy Atoms Enhances Its Thermal Stability. Acs Catalysis V. 6 3036 2016.
ISSN: ESSN 2155-5435
DOI: 10.1021/ACSCATAL.6B00100