Calcium in PDB 5bir: Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations

All present enzymatic activity of Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations:
3.1.27.3;

The structure of Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations, PDB code: 5bir was solved by J.Doumen, J.Steyaert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.00
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	58.390, 58.390, 133.390, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 25.5

The binding sites of Calcium atom in the Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations (pdb code 5bir). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations, PDB code: 5bir:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca501 b:27.7 occ:1.00 OE1 B:GLN85 2.4 10.8 1.0 O B:HOH626 2.6 25.6 1.0 CD B:GLN85 3.5 4.8 1.0 CG B:GLN85 4.1 6.7 1.0 NE2 B:GLN85 4.4 5.9 1.0 O B:HOH634 4.4 28.9 1.0 O B:HOH624 4.5 25.1 1.0 CB B:GLN85 4.6 11.4 1.0

Calcium binding site 2 out of 2 in the Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Disecting Histidine Interactions in Ribonuclease T1 Using Asn and Gln Mutations within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:18.7 occ:1.00 OD1 B:ASP49 2.2 20.0 1.0 O B:GLY47 2.3 11.4 1.0 CG B:ASP49 3.2 21.6 1.0 C B:GLY47 3.4 10.9 1.0 OD2 B:ASP49 3.5 31.8 1.0 CA B:GLY47 4.0 9.4 1.0 N B:PHE48 4.5 10.8 1.0 N B:ASP49 4.5 13.1 1.0 C B:PHE48 4.6 12.2 1.0 CB B:ASP49 4.6 19.1 1.0 CA B:PHE48 4.7 12.5 1.0 O B:PHE48 4.9 10.9 1.0 CA B:ASP49 4.9 17.0 1.0

S.De Vos, J.Doumen, U.Langhorst, J.Steyaert. Dissecting Histidine Interactions of Ribonuclease T1 with Asparagine and Glutamine Replacements: Analysis of Double Mutant Cycles at One Position. J.Mol.Biol. V. 275 651 1998.
ISSN: ISSN 0022-2836
PubMed: 9466938
DOI: 10.1006/JMBI.1997.1480