Calcium in PDB 5cv5: Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature

All present enzymatic activity of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature:
3.1.31.1;

The structure of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature, PDB code: 5cv5 was solved by G.Ortega Quintanilla, A.C.Robinson, J.L.Schlessman, B.Garcia-Moreno E., with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	30.831, 60.549, 77.901, 90.00, 93.15, 90.00
R / Rfree (%)	18.5 / 22.5

The binding sites of Calcium atom in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature (pdb code 5cv5). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature, PDB code: 5cv5:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca201 b:12.3 occ:1.00 OD1 A:ASP40 2.6 12.7 1.0 O A:THR41 2.8 10.7 1.0 OD2 A:ASP21 2.9 10.0 1.0 O A:HOH312 2.9 11.3 1.0 O A:HOH306 2.9 12.8 1.0 O5P A:THP202 3.1 13.0 1.0 OE2 A:GLU43 3.1 23.9 1.0 O A:HOH372 3.6 29.0 1.0 CG A:ASP21 3.8 9.6 1.0 CG A:ASP40 3.8 12.8 1.0 OD1 A:ASP21 3.8 9.0 1.0 N A:THR41 3.9 8.8 1.0 C A:THR41 3.9 10.3 1.0 OG1 A:THR41 3.9 9.7 1.0 O A:HOH331 3.9 11.5 1.0 NH2 A:ARG35 4.0 9.5 1.0 P2 A:THP202 4.0 14.3 1.0 O4P A:THP202 4.2 13.0 1.0 CD A:GLU43 4.2 25.6 1.0 CA A:THR41 4.4 9.2 1.0 O6P A:THP202 4.5 15.2 1.0 OD2 A:ASP40 4.5 14.8 1.0 C A:ASP40 4.6 8.9 1.0 CA A:ASP40 4.6 9.1 1.0 O A:HOH303 4.6 26.9 1.0 CZ A:ARG35 4.7 8.5 1.0 OE1 A:GLU43 4.8 25.2 1.0 C A:PRO42 4.8 12.6 1.0 CB A:THR41 4.8 9.2 1.0 CB A:ASP40 4.8 10.5 1.0 NE A:ARG35 4.8 8.5 1.0 O A:PRO42 4.9 13.7 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca201 b:15.7 occ:1.00 OD1 B:ASP40 2.7 19.0 1.0 O B:THR41 2.7 14.2 1.0 O B:HOH302 2.9 13.9 1.0 OE2 B:GLU43 2.9 35.4 1.0 O B:HOH317 2.9 11.9 1.0 OD2 B:ASP21 3.0 13.1 1.0 O4P B:THP202 3.1 18.2 1.0 O B:HOH338 3.5 31.4 1.0 O B:HOH301 3.7 14.4 1.0 CG B:ASP21 3.8 12.8 1.0 CG B:ASP40 3.8 17.8 1.0 OD1 B:ASP21 3.8 11.7 1.0 C B:THR41 3.9 13.2 1.0 N B:THR41 4.0 13.5 1.0 CD B:GLU43 4.0 31.1 1.0 P2 B:THP202 4.1 18.5 1.0 NH2 B:ARG35 4.1 13.9 1.0 OG1 B:THR41 4.1 11.6 1.0 O5P B:THP202 4.2 17.0 1.0 CA B:THR41 4.4 12.8 1.0 OD2 B:ASP40 4.5 20.5 1.0 CA B:ASP40 4.6 14.4 1.0 C B:ASP40 4.6 14.6 1.0 O B:HOH303 4.6 20.7 1.0 OE1 B:GLU43 4.7 32.4 1.0 O6P B:THP202 4.7 22.9 1.0 CZ B:ARG35 4.7 14.4 1.0 C B:PRO42 4.8 17.1 1.0 CB B:ASP40 4.8 15.6 1.0 CG B:GLU43 4.8 26.2 1.0 CB B:THR41 4.9 11.6 1.0 N B:GLU43 4.9 16.6 1.0 N B:PRO42 4.9 15.8 1.0 NE B:ARG35 5.0 13.7 1.0 O B:PRO42 5.0 14.9 1.0

G.Ortega Quintanilla, A.C.Robinson, J.L.Schlessman, B.Garcia-Moreno E.. Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs K64G/V66K/E67G at Cryogenic Temperature To Be Published.