Calcium in PDB 5d15: Crystal Structure of An Adenylyl Cyclase MA1120 From Mycobacterium Avium in Complex with Atp and Calcium Ion
Protein crystallography data
The structure of Crystal Structure of An Adenylyl Cyclase MA1120 From Mycobacterium Avium in Complex with Atp and Calcium Ion, PDB code: 5d15
was solved by
N.G.Bharambe,
D.V.Barathy,
K.Suguna,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
37.92 /
1.50
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
52.530,
55.640,
55.070,
90.00,
109.79,
90.00
|
R / Rfree (%)
|
19.5 /
21.2
|
Calcium Binding Sites:
The binding sites of Calcium atom in the Crystal Structure of An Adenylyl Cyclase MA1120 From Mycobacterium Avium in Complex with Atp and Calcium Ion
(pdb code 5d15). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the
Crystal Structure of An Adenylyl Cyclase MA1120 From Mycobacterium Avium in Complex with Atp and Calcium Ion, PDB code: 5d15:
Jump to Calcium binding site number:
1;
2;
Calcium binding site 1 out
of 2 in 5d15
Go back to
Calcium Binding Sites List in 5d15
Calcium binding site 1 out
of 2 in the Crystal Structure of An Adenylyl Cyclase MA1120 From Mycobacterium Avium in Complex with Atp and Calcium Ion
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Crystal Structure of An Adenylyl Cyclase MA1120 From Mycobacterium Avium in Complex with Atp and Calcium Ion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca302
b:19.5
occ:1.00
|
OD1
|
A:ASP113
|
2.3
|
32.8
|
1.0
|
O
|
A:ILE114
|
2.3
|
22.1
|
1.0
|
O2A
|
A:ATP301
|
2.3
|
21.8
|
1.0
|
O2B
|
A:ATP301
|
2.3
|
18.1
|
1.0
|
OD2
|
A:ASP157
|
2.3
|
30.4
|
1.0
|
O2G
|
A:ATP301
|
2.4
|
22.4
|
1.0
|
OD2
|
A:ASP113
|
2.6
|
26.2
|
1.0
|
CG
|
A:ASP113
|
2.8
|
26.4
|
1.0
|
O
|
A:HOH402
|
3.1
|
29.8
|
1.0
|
CG
|
A:ASP157
|
3.4
|
31.0
|
1.0
|
PB
|
A:ATP301
|
3.4
|
20.0
|
1.0
|
C
|
A:ILE114
|
3.5
|
20.1
|
1.0
|
PA
|
A:ATP301
|
3.5
|
26.0
|
1.0
|
HH12
|
A:ARG195
|
3.6
|
36.5
|
1.0
|
H
|
A:ILE114
|
3.6
|
22.2
|
1.0
|
PG
|
A:ATP301
|
3.6
|
21.3
|
1.0
|
O3A
|
A:ATP301
|
3.7
|
24.1
|
1.0
|
OD1
|
A:ASP157
|
3.8
|
33.9
|
1.0
|
H
|
A:SER117
|
3.8
|
22.1
|
1.0
|
N
|
A:ILE114
|
3.8
|
18.2
|
1.0
|
O3B
|
A:ATP301
|
3.9
|
20.4
|
1.0
|
H5'2
|
A:ATP301
|
4.0
|
48.9
|
1.0
|
HH11
|
A:ARG195
|
4.0
|
36.5
|
1.0
|
HG
|
A:SER117
|
4.1
|
24.7
|
1.0
|
H
|
A:GLU116
|
4.1
|
24.6
|
1.0
|
NH1
|
A:ARG195
|
4.1
|
25.0
|
1.0
|
HB3
|
A:SER117
|
4.2
|
24.0
|
1.0
|
O
|
A:HOH499
|
4.2
|
27.3
|
1.0
|
OG
|
A:SER117
|
4.2
|
19.7
|
1.0
|
HA
|
A:GLU115
|
4.2
|
23.7
|
1.0
|
CA
|
A:ILE114
|
4.2
|
18.7
|
1.0
|
CB
|
A:ASP113
|
4.2
|
19.8
|
1.0
|
HB
|
A:ILE114
|
4.3
|
26.1
|
1.0
|
O3G
|
A:ATP301
|
4.3
|
21.5
|
1.0
|
O
|
A:HOH515
|
4.3
|
37.6
|
1.0
|
C
|
A:ASP113
|
4.4
|
19.2
|
1.0
|
O5'
|
A:ATP301
|
4.5
|
29.6
|
1.0
|
N
|
A:GLU115
|
4.5
|
19.5
|
1.0
|
N
|
A:GLU116
|
4.6
|
21.9
|
1.0
|
O1A
|
A:ATP301
|
4.6
|
27.7
|
1.0
|
HA
|
A:ASP113
|
4.6
|
21.5
|
1.0
|
O1G
|
A:ATP301
|
4.7
|
19.8
|
1.0
|
HB2
|
A:ASP113
|
4.7
|
22.6
|
1.0
|
N
|
A:SER117
|
4.7
|
19.7
|
1.0
|
CB
|
A:ASP157
|
4.7
|
28.9
|
1.0
|
CB
|
A:SER117
|
4.7
|
19.7
|
1.0
|
CA
|
A:GLU115
|
4.7
|
21.4
|
1.0
|
HB2
|
A:ASP157
|
4.7
|
34.8
|
1.0
|
CA
|
A:ASP113
|
4.7
|
18.3
|
1.0
|
C5'
|
A:ATP301
|
4.7
|
40.7
|
1.0
|
O1B
|
A:ATP301
|
4.7
|
18.5
|
1.0
|
HB3
|
A:ASP113
|
4.8
|
22.6
|
1.0
|
CB
|
A:ILE114
|
4.8
|
20.7
|
1.0
|
C
|
A:GLU115
|
4.9
|
23.3
|
1.0
|
O
|
A:HOH501
|
4.9
|
42.6
|
1.0
|
O
|
A:HOH533
|
4.9
|
32.8
|
1.0
|
O
|
A:HOH490
|
5.0
|
39.4
|
1.0
|
|
Calcium binding site 2 out
of 2 in 5d15
Go back to
Calcium Binding Sites List in 5d15
Calcium binding site 2 out
of 2 in the Crystal Structure of An Adenylyl Cyclase MA1120 From Mycobacterium Avium in Complex with Atp and Calcium Ion
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Crystal Structure of An Adenylyl Cyclase MA1120 From Mycobacterium Avium in Complex with Atp and Calcium Ion within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca302
b:18.5
occ:1.00
|
O1B
|
B:ATP301
|
2.2
|
17.2
|
0.2
|
O1B
|
B:ATP301
|
2.2
|
17.6
|
0.8
|
OD1
|
B:ASP113
|
2.3
|
46.5
|
1.0
|
O
|
B:ILE114
|
2.3
|
28.1
|
1.0
|
OD2
|
B:ASP157
|
2.3
|
29.3
|
1.0
|
O1A
|
B:ATP301
|
2.4
|
33.6
|
0.2
|
O3G
|
B:ATP301
|
2.4
|
19.6
|
0.8
|
O1A
|
B:ATP301
|
2.4
|
29.5
|
0.8
|
O3G
|
B:ATP301
|
2.4
|
18.5
|
0.2
|
OD2
|
B:ASP113
|
2.4
|
40.4
|
1.0
|
CG
|
B:ASP113
|
2.7
|
37.7
|
1.0
|
PB
|
B:ATP301
|
3.4
|
17.9
|
0.2
|
PB
|
B:ATP301
|
3.4
|
18.0
|
0.8
|
O
|
B:HOH402
|
3.4
|
42.8
|
1.0
|
C
|
B:ILE114
|
3.4
|
25.9
|
1.0
|
HH12
|
B:ARG195
|
3.5
|
32.3
|
0.6
|
PA
|
B:ATP301
|
3.5
|
27.2
|
0.2
|
CG
|
B:ASP157
|
3.5
|
35.9
|
1.0
|
HG
|
B:SER117
|
3.6
|
19.5
|
1.0
|
PG
|
B:ATP301
|
3.6
|
17.7
|
0.2
|
PA
|
B:ATP301
|
3.6
|
30.4
|
0.8
|
PG
|
B:ATP301
|
3.6
|
21.2
|
0.8
|
H
|
B:ILE114
|
3.7
|
23.8
|
1.0
|
O3A
|
B:ATP301
|
3.7
|
22.0
|
0.8
|
H
|
B:SER117
|
3.8
|
20.2
|
1.0
|
O3B
|
B:ATP301
|
3.8
|
18.2
|
0.2
|
O3A
|
B:ATP301
|
3.8
|
21.1
|
0.2
|
N
|
B:ILE114
|
3.9
|
20.7
|
1.0
|
HH11
|
B:ARG195
|
3.9
|
32.3
|
0.6
|
HH21
|
B:ARG195
|
3.9
|
36.1
|
0.4
|
O3B
|
B:ATP301
|
3.9
|
17.4
|
0.8
|
NH1
|
B:ARG195
|
4.0
|
22.6
|
0.6
|
OD1
|
B:ASP157
|
4.1
|
38.3
|
1.0
|
HB3
|
B:SER117
|
4.1
|
18.8
|
1.0
|
O2A
|
B:ATP301
|
4.1
|
24.7
|
0.2
|
O1G
|
B:ATP301
|
4.1
|
18.7
|
0.2
|
CB
|
B:ASP113
|
4.2
|
27.0
|
1.0
|
HA
|
B:GLU115
|
4.2
|
23.7
|
1.0
|
OG
|
B:SER117
|
4.2
|
15.3
|
1.0
|
HE
|
B:ARG195
|
4.2
|
31.3
|
0.4
|
CA
|
B:ILE114
|
4.2
|
22.6
|
1.0
|
O1G
|
B:ATP301
|
4.3
|
19.1
|
0.8
|
O5'
|
B:ATP301
|
4.3
|
35.4
|
0.8
|
HB
|
B:ILE114
|
4.3
|
26.8
|
1.0
|
C
|
B:ASP113
|
4.4
|
21.1
|
1.0
|
H5'2
|
B:ATP301
|
4.4
|
49.5
|
0.8
|
N
|
B:GLU115
|
4.4
|
18.4
|
1.0
|
H5'1
|
B:ATP301
|
4.4
|
49.1
|
0.2
|
HB2
|
B:ASP157
|
4.5
|
58.2
|
1.0
|
N
|
B:GLU116
|
4.6
|
23.8
|
1.0
|
HA
|
B:ASP113
|
4.6
|
27.8
|
1.0
|
CB
|
B:SER117
|
4.6
|
14.9
|
1.0
|
HB2
|
B:ASP113
|
4.6
|
36.4
|
1.0
|
N
|
B:SER117
|
4.6
|
20.2
|
1.0
|
HB3
|
B:ASP113
|
4.7
|
36.4
|
1.0
|
CB
|
B:ASP157
|
4.7
|
39.4
|
1.0
|
CA
|
B:GLU115
|
4.7
|
21.2
|
1.0
|
CA
|
B:ASP113
|
4.7
|
21.9
|
1.0
|
NH2
|
B:ARG195
|
4.7
|
25.1
|
0.4
|
O2B
|
B:ATP301
|
4.7
|
16.4
|
0.2
|
O2B
|
B:ATP301
|
4.8
|
16.7
|
0.8
|
O2G
|
B:ATP301
|
4.8
|
17.3
|
0.8
|
O5'
|
B:ATP301
|
4.8
|
35.4
|
0.2
|
CB
|
B:ILE114
|
4.8
|
24.3
|
1.0
|
O2G
|
B:ATP301
|
4.9
|
17.5
|
0.2
|
C5'
|
B:ATP301
|
4.9
|
41.3
|
0.8
|
O2A
|
B:ATP301
|
4.9
|
25.4
|
0.8
|
C
|
B:GLU115
|
4.9
|
21.5
|
1.0
|
O
|
B:ASP113
|
5.0
|
20.9
|
1.0
|
|
Reference:
N.G.Bharambe,
D.V.Barathy,
W.Syed,
S.S.Visweswariah,
M.Cola Sigmaf O,
S.Misquith,
K.Suguna.
Substrate Specificity Determinants of Class III Nucleotidyl Cyclases Febs J. V. 283 3723 2016.
ISSN: ISSN 1742-464X
PubMed: 27542992
DOI: 10.1111/FEBS.13837
Page generated: Sun Jul 14 17:42:20 2024
|