Calcium in PDB 5e2j: Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius

All present enzymatic activity of Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius:
3.2.1.4;

The structure of Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius, PDB code: 5e2j was solved by Y.Y.Hsiao, H.J.Wang, C.P.Tseng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.96 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	55.421, 144.374, 158.704, 90.00, 90.00, 90.00
R / Rfree (%)	20.2 / 23.7

The structure of Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Calcium atom in the Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius (pdb code 5e2j). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius, PDB code: 5e2j:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:41.3 occ:1.00 OE1 A:GLU308 2.4 50.0 1.0 OD2 A:ASP302 2.4 41.3 1.0 OD1 A:ASP307 2.4 35.2 1.0 OD2 A:ASP307 2.4 34.9 1.0 O A:GLU304 2.4 43.0 1.0 O A:ALA344 2.4 46.0 1.0 O A:HOH807 2.4 39.5 1.0 OE2 A:GLU308 2.5 47.5 1.0 CG A:ASP307 2.8 39.7 1.0 CD A:GLU308 2.8 47.0 1.0 CG A:ASP302 3.5 44.7 1.0 C A:GLU304 3.6 42.3 1.0 C A:ALA344 3.6 44.7 1.0 OD1 A:ASP302 3.9 42.1 1.0 O A:HOH772 4.0 39.6 1.0 CB A:ASP307 4.3 40.5 1.0 CG A:GLU308 4.3 47.4 1.0 CA A:ASP345 4.3 37.8 1.0 CA A:LEU305 4.4 44.0 1.0 N A:LEU305 4.4 40.0 1.0 N A:ASP345 4.4 37.7 1.0 CB A:GLU304 4.5 41.0 1.0 N A:GLU308 4.6 41.3 1.0 CA A:GLU304 4.6 42.9 1.0 OD1 A:ASP345 4.6 46.1 1.0 CG A:GLU304 4.6 38.1 1.0 CA A:ALA344 4.7 44.4 1.0 CB A:ASP302 4.8 47.0 1.0 O A:HOH737 4.8 40.5 1.0 N A:VAL346 4.9 33.1 1.0 N A:ASP307 4.9 44.6 1.0 C A:LEU305 4.9 48.4 1.0 CA A:ASP307 5.0 41.9 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Single Mutant Thermostable Endoglucanase (D468A) From Alicyclobacillus Acidocaldarius within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:55.9 occ:1.00 OD2 B:ASP302 2.4 56.9 1.0 OD1 B:ASP307 2.4 54.6 1.0 OD2 B:ASP307 2.4 57.9 1.0 O B:ALA344 2.4 50.4 1.0 O B:GLU304 2.5 62.2 1.0 O B:HOH744 2.6 74.2 1.0 OE2 B:GLU308 2.6 54.5 1.0 OE1 B:GLU308 2.7 60.5 1.0 CG B:ASP307 2.7 56.8 1.0 CD B:GLU308 3.0 60.4 1.0 CG B:ASP302 3.4 56.4 1.0 C B:ALA344 3.5 49.2 1.0 OD1 B:ASP302 3.7 57.7 1.0 C B:GLU304 3.7 61.3 1.0 CA B:ASP345 4.1 50.4 1.0 O B:HOH711 4.1 49.7 1.0 CB B:ASP307 4.3 59.2 1.0 N B:ASP345 4.3 47.5 1.0 CA B:LEU305 4.4 61.1 1.0 OD1 B:ASP345 4.4 52.9 1.0 N B:LEU305 4.4 63.1 1.0 CG B:GLU308 4.5 62.6 1.0 CA B:ALA344 4.6 47.3 1.0 CB B:GLU304 4.6 58.4 1.0 CA B:GLU304 4.7 61.3 1.0 CB B:ASP302 4.7 54.7 1.0 CG B:GLU304 4.7 61.2 1.0 N B:VAL346 4.8 50.5 1.0 N B:GLU308 4.8 65.0 1.0 O B:HOH742 4.9 48.5 1.0 N B:ASP307 4.9 60.5 1.0 C B:LEU305 5.0 60.4 1.0 CG B:ASP345 5.0 53.4 1.0

H.J.Wang, Y.Y.Hsiao, Y.P.Chen, T.Y.Ma, C.P.Tseng. Polarity Alteration of A Calcium Site Induces A Hydrophobic Interaction Network and Enhances CEL9A Endoglucanase Thermostability. Appl.Environ.Microbiol. V. 82 1662 2016.
ISSN: ESSN 1098-5336
PubMed: 26729722
DOI: 10.1128/AEM.03326-15