Calcium in PDB 5e8i: Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg

The structure of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg, PDB code: 5e8i was solved by C.Hou, O.V.Tsodikov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.00 / 3.45
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	86.647, 86.647, 230.282, 90.00, 90.00, 120.00
R / Rfree (%)	22.2 / 27.1

The binding sites of Calcium atom in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg (pdb code 5e8i). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg, PDB code: 5e8i:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Calcium binding site 1 out of 8 in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca101 b:0.7 occ:1.00 O6 B:DG9 3.0 0.3 1.0 N7 B:DA8 3.9 0.6 1.0 N7 B:DG9 3.9 0.9 1.0 C6 B:DG9 4.0 0.0 1.0 C5 B:DG9 4.3 0.7 1.0 C8 B:DA8 4.5 0.4 1.0 OE2 D:GLU303 4.6 0.7 1.0 C5 B:DA8 4.6 0.8 1.0 N6 B:DA8 4.7 0.3 1.0

Calcium binding site 2 out of 8 in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca101 b:0.1 occ:1.00 N7 C:DG21 3.0 0.3 1.0 O6 C:DG21 3.9 0.0 1.0 C8 C:DG21 3.9 0.2 1.0 C5 C:DG21 4.0 0.7 1.0 O6 C:DG22 4.1 0.5 1.0 C6 C:DG21 4.3 0.0 1.0 C2' C:DC20 4.9 0.9 1.0 C6 C:DC20 4.9 0.2 1.0 C6 C:DG22 5.0 0.4 1.0 N7 C:DG22 5.0 0.2 1.0

Calcium binding site 3 out of 8 in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca102 b:0.1 occ:1.00 N2 C:DG21 4.0 0.7 1.0 N3 C:DG21 4.5 0.8 1.0 C1' C:DG22 4.5 0.5 1.0 O4' C:DG22 4.5 0.8 1.0 C2 C:DG21 4.8 0.5 1.0

Calcium binding site 4 out of 8 in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg within 5.0Å range: probe atom residue distance (Å) B Occ F:Ca101 b:0.3 occ:1.00 N7 F:DG21 3.1 0.7 1.0 C8 F:DG21 3.8 0.6 1.0 C5 F:DG21 4.1 0.6 1.0 O6 F:DG22 4.3 0.8 1.0 O6 F:DG21 4.4 0.5 1.0 C2' F:DC20 4.6 0.3 1.0 OP2 F:DG21 4.6 0.5 1.0 C6 F:DG21 4.7 0.0 1.0 C6 F:DC20 4.9 0.6 1.0 N7 F:DG22 4.9 0.3 1.0

Calcium binding site 5 out of 8 in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg within 5.0Å range: probe atom residue distance (Å) B Occ F:Ca102 b:0.7 occ:1.00 N2 F:DG21 4.0 0.6 1.0 N3 F:DG21 4.4 0.2 1.0 O4' E:DG6 4.5 0.5 1.0 N2 E:DG5 4.6 0.4 1.0 C2 F:DG21 4.7 0.6 1.0 O4' F:DG22 4.7 0.6 1.0 C4' F:DG22 5.0 0.4 1.0 C5' F:DG22 5.0 0.5 1.0

Calcium binding site 6 out of 8 in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg within 5.0Å range: probe atom residue distance (Å) B Occ H:Ca101 b:0.6 occ:1.00 N2 I:DG21 4.0 0.8 1.0 O4' H:DG6 4.2 1.0 1.0 N2 H:DG5 4.5 0.0 1.0 C4' H:DG6 4.8 0.9 1.0 N3 I:DG21 4.8 0.6 1.0 C2 I:DG21 4.9 0.2 1.0 N3 H:DG5 5.0 0.2 1.0

Calcium binding site 7 out of 8 in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg within 5.0Å range: probe atom residue distance (Å) B Occ I:Ca101 b:0.3 occ:1.00 N7 I:DG21 3.8 0.3 1.0 O6 I:DG22 3.8 0.5 1.0 O6 I:DG21 4.5 0.2 1.0 C8 I:DG21 4.6 0.1 1.0 C5 I:DG21 4.7 0.2 1.0 C6 I:DG22 4.8 0.1 1.0 C6 I:DG21 5.0 0.4 1.0 N7 I:DG22 5.0 0.6 1.0

Calcium binding site 8 out of 8 in the Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Crystal Structure of the Dna Binding Domain of Human Transcription Factor FLI1 in Complex with A 10-Mer Dna Accggaagtg within 5.0Å range: probe atom residue distance (Å) B Occ L:Ca101 b:0.4 occ:1.00 N2 L:DG21 3.8 0.5 1.0 N3 L:DG21 4.3 0.1 1.0 C2 L:DG21 4.5 0.3 1.0 O4' L:DG22 4.7 0.7 1.0 C1' L:DG22 4.7 0.6 1.0 N2 K:DG5 4.8 0.8 1.0 O4' K:DG6 4.9 0.8 1.0

C.Hou, O.V.Tsodikov. Structural Basis For Dimerization and Dna Binding of Transcription Factor FLI1. Biochemistry V. 54 7365 2015.
ISSN: ISSN 0006-2960
PubMed: 26618620
DOI: 10.1021/ACS.BIOCHEM.5B01121