Calcium in PDB 5ewe: Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G
Enzymatic activity of Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G
All present enzymatic activity of Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G:
2.7.7.7;
Protein crystallography data
The structure of Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G, PDB code: 5ewe
was solved by
Y.Su,
M.Egli,
F.P.Guengerich,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
42.99 /
1.66
|
Space group
|
P 61
|
Cell size a, b, c (Å), α, β, γ (°)
|
99.282,
99.282,
81.895,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
17.1 /
20.7
|
Calcium Binding Sites:
The binding sites of Calcium atom in the Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G
(pdb code 5ewe). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the
Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G, PDB code: 5ewe:
Jump to Calcium binding site number:
1;
2;
Calcium binding site 1 out
of 2 in 5ewe
Go back to
Calcium Binding Sites List in 5ewe
Calcium binding site 1 out
of 2 in the Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca502
b:11.8
occ:1.00
|
OD1
|
A:ASP13
|
2.3
|
20.4
|
1.0
|
O1B
|
A:CTP501
|
2.3
|
11.4
|
1.0
|
O
|
A:MET14
|
2.3
|
13.3
|
1.0
|
OD2
|
A:ASP115
|
2.3
|
14.2
|
1.0
|
O1G
|
A:CTP501
|
2.3
|
13.5
|
1.0
|
O1A
|
A:CTP501
|
2.4
|
20.2
|
1.0
|
CA
|
A:CA503
|
3.4
|
22.9
|
0.2
|
PB
|
A:CTP501
|
3.4
|
12.7
|
1.0
|
CG
|
A:ASP13
|
3.4
|
24.8
|
1.0
|
CG
|
A:ASP115
|
3.4
|
16.8
|
1.0
|
C
|
A:MET14
|
3.5
|
12.3
|
1.0
|
PG
|
A:CTP501
|
3.6
|
13.2
|
1.0
|
PA
|
A:CTP501
|
3.6
|
16.2
|
1.0
|
O3A
|
A:CTP501
|
3.8
|
17.2
|
1.0
|
O3B
|
A:CTP501
|
3.8
|
13.7
|
1.0
|
O
|
A:HOH638
|
3.9
|
32.8
|
1.0
|
OD1
|
A:ASP115
|
3.9
|
18.7
|
1.0
|
OD2
|
A:ASP13
|
3.9
|
35.1
|
1.0
|
N
|
A:MET14
|
4.0
|
12.6
|
1.0
|
NZ
|
A:LYS231
|
4.1
|
18.8
|
1.0
|
C5'
|
A:CTP501
|
4.2
|
17.5
|
1.0
|
CA
|
A:MET14
|
4.3
|
11.3
|
1.0
|
O2G
|
A:CTP501
|
4.3
|
14.3
|
1.0
|
C
|
A:ASP13
|
4.3
|
13.1
|
1.0
|
O5'
|
A:CTP501
|
4.4
|
18.6
|
1.0
|
N
|
A:ASP15
|
4.5
|
10.2
|
1.0
|
CB
|
A:ASP13
|
4.6
|
14.0
|
1.0
|
N
|
A:CYS16
|
4.6
|
11.4
|
1.0
|
CA
|
A:ASP15
|
4.7
|
10.5
|
1.0
|
CB
|
A:ASP115
|
4.7
|
14.6
|
1.0
|
CB
|
A:MET14
|
4.7
|
12.0
|
1.0
|
O2B
|
A:CTP501
|
4.8
|
13.8
|
1.0
|
O
|
A:ASP13
|
4.8
|
12.5
|
1.0
|
O3G
|
A:CTP501
|
4.8
|
14.2
|
1.0
|
O2A
|
A:CTP501
|
4.8
|
18.5
|
1.0
|
C
|
A:ASP15
|
4.8
|
12.0
|
1.0
|
CA
|
A:ASP13
|
4.8
|
14.8
|
1.0
|
CE
|
A:LYS231
|
4.8
|
26.9
|
1.0
|
N
|
A:PHE17
|
4.9
|
9.5
|
1.0
|
O
|
A:ASP115
|
4.9
|
13.8
|
1.0
|
CB
|
A:PHE17
|
4.9
|
10.4
|
1.0
|
O
|
A:HOH855
|
5.0
|
30.6
|
1.0
|
|
Calcium binding site 2 out
of 2 in 5ewe
Go back to
Calcium Binding Sites List in 5ewe
Calcium binding site 2 out
of 2 in the Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Ternary Complex of Human Dna Polymerase Eta Inserting Rctp Opposite Template G within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca503
b:22.9
occ:0.25
|
O3'
|
P:DT8
|
2.3
|
24.2
|
0.7
|
O3'
|
P:DT8
|
2.3
|
24.4
|
0.3
|
OE2
|
A:GLU116
|
2.5
|
32.7
|
1.0
|
O1A
|
A:CTP501
|
2.5
|
20.2
|
1.0
|
OD1
|
A:ASP115
|
2.6
|
18.7
|
1.0
|
C3'
|
P:DT8
|
2.8
|
24.1
|
0.3
|
C3'
|
P:DT8
|
2.9
|
24.6
|
0.7
|
CG
|
A:GLU116
|
2.9
|
24.1
|
1.0
|
CD
|
A:GLU116
|
3.0
|
24.0
|
1.0
|
OD1
|
A:ASP13
|
3.1
|
20.4
|
1.0
|
O
|
A:HOH638
|
3.2
|
32.8
|
1.0
|
OD2
|
A:ASP13
|
3.2
|
35.1
|
1.0
|
CG
|
A:ASP115
|
3.3
|
16.8
|
1.0
|
CA
|
A:CA502
|
3.4
|
11.8
|
1.0
|
OD2
|
A:ASP115
|
3.4
|
14.2
|
1.0
|
CG
|
A:ASP13
|
3.4
|
24.8
|
1.0
|
PA
|
A:CTP501
|
3.7
|
16.2
|
1.0
|
C4'
|
P:DT8
|
3.8
|
20.5
|
0.3
|
C2'
|
P:DT8
|
3.9
|
22.4
|
0.7
|
C2'
|
P:DT8
|
3.9
|
22.3
|
0.3
|
O5'
|
A:CTP501
|
4.0
|
18.6
|
1.0
|
OG
|
A:SER113
|
4.0
|
19.7
|
1.0
|
C4'
|
P:DT8
|
4.1
|
20.4
|
0.7
|
C5'
|
A:CTP501
|
4.1
|
17.5
|
1.0
|
OE1
|
A:GLU116
|
4.2
|
25.8
|
1.0
|
CB
|
A:GLU116
|
4.2
|
17.9
|
1.0
|
O2A
|
A:CTP501
|
4.4
|
18.5
|
1.0
|
O
|
A:ASP115
|
4.5
|
13.8
|
1.0
|
C5'
|
P:DT8
|
4.6
|
23.4
|
0.3
|
C
|
A:ASP115
|
4.6
|
16.1
|
1.0
|
CB
|
A:ASP13
|
4.7
|
14.0
|
1.0
|
CB
|
A:ASP115
|
4.7
|
14.6
|
1.0
|
N
|
A:GLU116
|
4.8
|
14.0
|
1.0
|
O1G
|
A:CTP501
|
4.8
|
13.5
|
1.0
|
C5'
|
P:DT8
|
4.8
|
24.7
|
0.7
|
O1B
|
A:CTP501
|
4.9
|
11.4
|
1.0
|
CA
|
A:GLU116
|
5.0
|
14.1
|
1.0
|
C1'
|
P:DT8
|
5.0
|
19.3
|
0.3
|
O4'
|
P:DT8
|
5.0
|
20.7
|
0.3
|
|
Reference:
Y.Su,
M.Egli,
F.P.Guengerich.
Mechanism of Ribonucleotide Incorporation By Human Dna Polymerase Eta. J.Biol.Chem. V. 291 3747 2016.
ISSN: ESSN 1083-351X
PubMed: 26740629
DOI: 10.1074/JBC.M115.706226
Page generated: Sat Dec 12 05:24:08 2020
|