Atomistry » Calcium » PDB 5eym-5fk0 » 5f2g
Atomistry »
  Calcium »
    PDB 5eym-5fk0 »
      5f2g »

Calcium in PDB 5f2g: Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp

Enzymatic activity of Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp

All present enzymatic activity of Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp:
4.1.2.40;

Protein crystallography data

The structure of Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp, PDB code: 5f2g was solved by C.Lowkam, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.73 / 1.92
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 63.712, 106.858, 238.445, 90.00, 90.00, 90.00
R / Rfree (%) 15.5 / 19.6

Calcium Binding Sites:

The binding sites of Calcium atom in the Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp (pdb code 5f2g). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 5 binding sites of Calcium where determined in the Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp, PDB code: 5f2g:
Jump to Calcium binding site number: 1; 2; 3; 4; 5;

Calcium binding site 1 out of 5 in 5f2g

Go back to Calcium Binding Sites List in 5f2g
Calcium binding site 1 out of 5 in the Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca3002

b:53.5
occ:1.00
 O A:HOH3450 2.6 42.1 1.0
O B:HOH618 2.6 43.5 1.0
O A:HOH3115 2.6 47.5 1.0
O A:HOH3347 2.6 35.9 1.0
O A:HOH3235 2.7 45.6 1.0
O A:HOH3358 2.7 29.0 1.0
HB2 A:GLU197 3.7 28.7 1.0
O A:HOH3431 3.7 62.4 1.0
H A:GLU197 4.1 20.4 1.0
HD1 B:PHE63 4.2 37.6 1.0
HA B:PHE63 4.3 28.2 1.0
O A:HOH3228 4.4 27.6 1.0
O A:HOH3499 4.4 30.0 1.0
O A:HOH3101 4.4 61.8 1.0
O B:PRO62 4.5 27.3 1.0
CB A:GLU197 4.6 23.9 1.0
N A:GLU197 4.7 17.0 1.0
O A:HOH3412 4.7 38.7 1.0
HA A:LYS196 4.7 20.8 1.0
O A:HOH3483 4.8 34.4 1.0
O A:SER195 4.8 21.8 1.0
O B:HOH704 4.9 40.7 1.0
O B:HOH885 4.9 28.9 1.0
OD2 A:ASP242 4.9 62.1 0.5
OE1 A:GLU197 4.9 90.8 1.0
HB3 A:GLU197 5.0 28.7 1.0
HA A:GLU197 5.0 24.2 1.0

Calcium binding site 2 out of 5 in 5f2g

Go back to Calcium Binding Sites List in 5f2g
Calcium binding site 2 out of 5 in the Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca3003

b:42.5
occ:1.00
 O A:GLU238 2.3 23.5 1.0
O A:THR241 2.4 19.4 1.0
OE2 B:GLU84 2.4 42.0 1.0
OE1 B:GLU83 2.6 50.5 1.0
O A:HOH3139 2.6 49.3 1.0
OE2 A:GLU238 2.6 54.0 1.0
HA A:ASP242 3.3 30.9 0.5
O A:HOH3223 3.4 33.0 1.0
HA A:ASP242 3.4 32.2 0.5
CD A:GLU238 3.5 43.5 1.0
HG3 A:GLU238 3.5 43.2 1.0
C A:GLU238 3.5 28.2 1.0
C A:THR241 3.5 22.4 1.0
CD B:GLU84 3.6 40.3 1.0
CD B:GLU83 3.6 85.5 1.0
HA A:ALA239 3.7 28.4 1.0
HG3 B:GLU83 3.8 57.4 1.0
H A:THR241 3.8 27.2 0.6
H A:THR241 3.8 27.2 0.4
CG A:GLU238 4.0 36.0 1.0
HG2 B:GLU83 4.0 57.4 1.0
CG B:GLU83 4.0 47.8 1.0
CA A:ASP242 4.1 25.7 0.5
CA A:ASP242 4.2 26.8 0.5
HA A:GLU238 4.2 27.1 1.0
HZ1 A:LYS325 4.2 63.3 0.9
HG23 A:THR241 4.3 30.1 1.0
N A:ASP242 4.3 24.8 0.5
N A:ASP242 4.3 21.0 0.5
OE1 B:GLU84 4.3 50.0 1.0
HG3 B:GLU84 4.3 30.3 1.0
OE1 A:GLU238 4.4 41.9 1.0
N A:ALA239 4.4 23.9 1.0
CA A:GLU238 4.4 22.6 1.0
CA A:ALA239 4.4 23.7 1.0
O A:HOH3381 4.5 42.9 1.0
N A:THR241 4.5 22.7 1.0
CG B:GLU84 4.5 25.3 1.0
CA A:THR241 4.6 20.8 1.0
HE1 A:TYR245 4.6 28.9 1.0
OE2 B:GLU83 4.7 57.5 1.0
OD1 A:ASP242 4.7 35.2 0.5
HG2 A:GLU238 4.8 43.2 1.0
HB3 A:ASP242 4.8 37.3 0.5
HG22 A:THR241 4.8 30.1 1.0
HG2 B:GLU84 4.8 30.3 1.0
CB A:GLU238 4.8 23.2 1.0
C A:ALA239 4.8 22.1 1.0
HZ2 A:LYS325 4.9 63.3 0.9
CG2 A:THR241 4.9 25.1 1.0
HB2 A:ASP242 4.9 40.9 0.5
H A:SER240 5.0 24.6 0.6
NZ A:LYS325 5.0 52.7 0.9
H A:SER240 5.0 26.3 0.4

Calcium binding site 3 out of 5 in 5f2g

Go back to Calcium Binding Sites List in 5f2g
Calcium binding site 3 out of 5 in the Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca402

b:62.9
occ:0.78
 O B:GLN40 2.6 81.9 1.0
O B:ALA37 2.8 80.8 1.0
O B:HOH603 2.8 68.3 1.0
O B:HOH507 2.9 42.5 1.0
HA B:THR41 3.1 93.1 1.0
O B:THR41 3.4 75.0 1.0
C B:GLN40 3.7 78.7 1.0
HB1 B:ALA37 3.8 84.3 1.0
CA B:THR41 3.8 77.6 1.0
C B:THR41 3.8 74.8 1.0
C B:ALA37 3.9 71.5 1.0
N B:THR41 4.2 75.7 1.0
HA B:ALA37 4.3 81.4 1.0
H B:GLN40 4.3 86.2 1.0
CA B:ALA37 4.5 67.8 1.0
O B:HOH888 4.5 54.6 1.0
CB B:ALA37 4.6 70.2 1.0
O B:LYS42 4.7 70.8 1.0
HG3 B:GLN40 4.8 79.0 1.0
HA B:GLN38 4.8 0.1 1.0
N B:LYS42 4.9 73.0 1.0
CA B:GLN40 5.0 72.4 1.0
N B:GLN40 5.0 71.8 1.0

Calcium binding site 4 out of 5 in 5f2g

Go back to Calcium Binding Sites List in 5f2g
Calcium binding site 4 out of 5 in the Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca403

b:49.5
occ:1.00
 O B:GLU238 2.4 24.3 1.0
O B:THR241 2.4 19.1 1.0
OE2 B:GLU238 2.7 47.8 1.0
O B:HOH633 3.1 36.8 1.0
HA B:ASP242 3.3 31.4 0.5
HA B:ASP242 3.4 32.9 0.6
CD B:GLU238 3.5 39.2 1.0
HG3 B:GLU238 3.6 35.4 1.0
C B:GLU238 3.6 23.8 1.0
C B:THR241 3.6 24.0 1.0
HA B:ALA239 3.8 24.5 1.0
H B:THR241 3.9 26.8 0.6
H B:THR241 3.9 26.8 0.4
CG B:GLU238 4.1 29.5 1.0
HG23 B:THR241 4.2 30.3 1.0
CA B:ASP242 4.2 26.1 0.5
HA B:GLU238 4.3 25.2 1.0
CA B:ASP242 4.3 27.4 0.6
HE3 B:LYS325 4.3 96.6 0.9
HE2 B:LYS325 4.3 96.6 0.9
N B:ASP242 4.3 24.4 0.5
N B:ASP242 4.4 21.7 0.6
OE1 B:GLU238 4.4 38.1 1.0
CA B:GLU238 4.5 21.0 1.0
N B:ALA239 4.5 21.6 1.0
CA B:ALA239 4.5 20.4 1.0
N B:THR241 4.6 22.3 1.0
HZ1 B:LYS325 4.6 52.6 0.9
HE1 B:TYR245 4.7 28.6 1.0
CA B:THR241 4.7 23.2 1.0
HG22 B:THR241 4.7 30.3 1.0
CE B:LYS325 4.7 80.5 0.9
HG2 B:GLU238 4.8 35.4 1.0
HB3 B:ASP242 4.9 46.4 0.6
CG2 B:THR241 4.9 25.2 1.0
CB B:GLU238 4.9 19.8 1.0
C B:ALA239 4.9 19.8 1.0
OD1 B:ASP242 5.0 63.1 0.5
CG B:ASP242 5.0 58.4 0.5

Calcium binding site 5 out of 5 in 5f2g

Go back to Calcium Binding Sites List in 5f2g
Calcium binding site 5 out of 5 in the Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Tagatose-1,6-Bisphosphate Aldolase From Streptococcus Pyogenes GLU164GLN Mutant in Complex with Fbp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca404

b:62.5
occ:0.75
 O C:HOH674 2.5 41.6 1.0
O B:HOH644 2.5 40.6 1.0
O B:HOH846 2.6 56.6 1.0
O B:HOH793 2.8 0.8 1.0
O B:HOH902 3.0 56.9 1.0
O A:HOH3525 3.2 63.5 1.0
O B:HOH691 3.7 53.3 1.0
O B:HOH860 4.0 70.8 1.0
HA B:LYS317 4.2 25.9 1.0
O B:ALA319 4.4 22.4 1.0
O B:HOH760 4.5 64.0 1.0
O B:LYS317 4.5 26.1 1.0
O B:ASP316 4.6 38.7 1.0
C B:LYS317 4.6 21.1 1.0
O B:HOH636 4.8 79.0 1.0
O C:HOH415 4.8 33.7 1.0
CA B:LYS317 4.9 21.6 1.0
O C:HOH662 4.9 35.2 1.0

Reference:

C.Lowkam, B.Liotard, J.Sygusch. Non-Stereospecific Substrate Cleavage By Tagatose-Bisphosphate Class I Aldolase To Be Published.
Page generated: Wed Jul 9 05:48:35 2025

Last articles

Cl in 5H0Z
Cl in 5H0Y
Cl in 5GZK
Cl in 5GXZ
Cl in 5GWF
Cl in 5GXY
Cl in 5GXX
Cl in 5GWI
Cl in 5GWJ
Cl in 5GWB
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy