Calcium in PDB 5gib: Succinic Acid Bound Trypsin Crystallized As Dimer

All present enzymatic activity of Succinic Acid Bound Trypsin Crystallized As Dimer:
3.4.21.4;

The structure of Succinic Acid Bound Trypsin Crystallized As Dimer, PDB code: 5gib was solved by N.H.V.Kutumbarao, R.Manohar, L.Karthik, P.Malathy, K.Gunasekaran, D.Velmurugan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	53.58 / 2.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.290, 58.260, 136.470, 90.00, 90.00, 90.00
R / Rfree (%)	22.9 / 28.6

The binding sites of Calcium atom in the Succinic Acid Bound Trypsin Crystallized As Dimer (pdb code 5gib). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Succinic Acid Bound Trypsin Crystallized As Dimer, PDB code: 5gib:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Succinic Acid Bound Trypsin Crystallized As Dimer


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Succinic Acid Bound Trypsin Crystallized As Dimer within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca302 b:27.8 occ:1.00 OE2 A:GLU62 2.5 24.5 1.0 O A:ASN54 2.5 20.3 1.0 OE2 A:GLU52 2.5 23.7 1.0 O A:VAL57 2.5 24.1 1.0 CD A:GLU62 3.4 28.9 1.0 CG A:GLU62 3.5 19.5 1.0 C A:ASN54 3.5 16.3 1.0 C A:VAL57 3.6 19.1 1.0 CD A:GLU52 3.7 18.2 1.0 N A:GLU59 3.8 16.3 1.0 OE1 A:GLU59 3.9 12.8 1.0 CG A:GLU59 4.0 28.8 1.0 CA A:VAL58 4.0 7.7 1.0 N A:VAL58 4.2 10.3 1.0 OE1 A:GLU52 4.3 26.7 1.0 CB A:GLU59 4.3 32.7 1.0 CA A:ILE55 4.3 15.7 1.0 N A:ILE55 4.4 21.1 1.0 C A:VAL58 4.4 10.4 1.0 CD A:GLU59 4.4 26.0 1.0 N A:ASN54 4.4 6.2 1.0 CA A:ASN54 4.5 8.0 1.0 OE1 A:GLU62 4.5 24.2 1.0 N A:VAL57 4.6 14.2 1.0 CA A:VAL57 4.7 18.4 1.0 CA A:GLU59 4.7 16.8 1.0 CG A:GLU52 4.8 9.0 1.0 N A:ASP53 4.8 8.8 1.0 CB A:ASN54 4.8 12.9 1.0 C A:ILE55 4.9 11.4 1.0 CA A:GLU52 4.9 9.9 1.0 CB A:GLU62 5.0 11.7 1.0 CB A:GLU52 5.0 11.1 1.0

Calcium binding site 2 out of 2 in the Succinic Acid Bound Trypsin Crystallized As Dimer


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Succinic Acid Bound Trypsin Crystallized As Dimer within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca301 b:38.8 occ:1.00 O B:ASN54 2.4 16.4 1.0 OE2 B:GLU62 2.4 21.9 1.0 O B:VAL57 2.6 16.5 1.0 OE2 B:GLU59 2.9 32.4 1.0 OE2 B:GLU52 2.9 15.3 1.0 CD B:GLU62 3.3 22.8 1.0 C B:ASN54 3.4 19.2 1.0 CG B:GLU62 3.5 16.6 1.0 CD B:GLU59 3.6 29.5 1.0 N B:ASN54 3.6 16.2 1.0 CG B:GLU59 3.8 30.2 1.0 C B:VAL57 3.8 16.8 1.0 CD B:GLU52 3.8 21.2 1.0 O B:HOH411 3.9 22.3 1.0 CA B:ASN54 3.9 13.0 1.0 N B:GLU59 4.1 24.1 1.0 CB B:GLU59 4.1 32.2 1.0 N B:ASP53 4.1 20.4 1.0 CB B:ASN54 4.2 14.3 1.0 OE1 B:GLU62 4.3 18.8 1.0 OE1 B:GLU52 4.5 10.2 1.0 N B:ILE55 4.5 17.1 1.0 CA B:VAL58 4.5 13.9 1.0 CA B:GLU52 4.5 17.0 1.0 OE1 B:GLU59 4.6 14.5 1.0 N B:VAL58 4.6 17.8 1.0 C B:VAL58 4.7 15.7 1.0 CG B:GLU52 4.7 28.8 1.0 CB B:GLU52 4.7 14.2 1.0 C B:ASP53 4.8 19.5 1.0 CA B:GLU59 4.8 27.2 1.0 CA B:VAL57 4.8 15.0 1.0 N B:VAL57 4.9 14.2 1.0 C B:GLU52 4.9 19.7 1.0 CA B:ILE55 4.9 14.5 1.0

N.H.V.Kutumbarao, R.Manohar, L.Karthik, P.Malathy, K.Gunasekaran, D.Velmurugan. Trypsin Bound with Succinic Acid To Be Published.