Calcium in PDB 5hoh: Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp

All present enzymatic activity of Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp:
3.1.27.3;

The structure of Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp, PDB code: 5hoh was solved by U.Langhorst, R.Loris, V.P.Denisov, J.Doumen, P.Roose, D.Maes, B.Halle, J.Steyaert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.350, 60.510, 101.150, 90.00, 90.00, 90.00
R / Rfree (%)	16.6 / 21.2

The binding sites of Calcium atom in the Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp (pdb code 5hoh). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp, PDB code: 5hoh:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca305 b:12.0 occ:1.00 OD2 C:ASP66 4.6 34.8 1.0 O C:SER64 4.7 29.7 1.0 OD1 C:ASP66 4.8 35.4 1.0

Calcium binding site 2 out of 2 in the Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Ribonuclease T1 (ASN9ALA/THR93ALA Doublemutant) Complexed with 2'Gmp within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca306 b:21.4 occ:1.00 O D:GLY47 2.4 9.1 1.0 OD1 D:ASP49 2.5 26.1 1.0 C D:GLY47 3.5 11.1 1.0 CG D:ASP49 3.5 27.7 1.0 OD2 D:ASP49 3.8 33.3 1.0 CA D:GLY47 4.1 9.6 1.0 N D:PHE48 4.6 10.1 1.0 N D:ASP49 4.6 11.6 1.0 C D:PHE48 4.7 9.9 1.0 CA D:PHE48 4.8 10.3 1.0 CB D:ASP49 4.9 20.2 1.0

U.Langhorst, R.Loris, V.P.Denisov, J.Doumen, P.Roose, D.Maes, B.Halle, J.Steyaert. Dissection of the Structural and Functional Role of A Conserved Hydration Site in Rnase T1. Protein Sci. V. 8 722 1999.
ISSN: ISSN 0961-8368
PubMed: 10211818