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Calcium in PDB 5hsa: Alcohol Oxidase AOX1 From Pichia Pastoris

Enzymatic activity of Alcohol Oxidase AOX1 From Pichia Pastoris

All present enzymatic activity of Alcohol Oxidase AOX1 From Pichia Pastoris:
1.1.3.13;

Protein crystallography data

The structure of Alcohol Oxidase AOX1 From Pichia Pastoris, PDB code: 5hsa was solved by P.Neumann, R.Ficner, I.Feussner, C.Koch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.88 / 2.35
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 117.100, 165.190, 164.310, 90.00, 95.67, 90.00
R / Rfree (%) 17.7 / 20.5

Other elements in 5hsa:

The structure of Alcohol Oxidase AOX1 From Pichia Pastoris also contains other interesting chemical elements:

Chlorine (Cl) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the Alcohol Oxidase AOX1 From Pichia Pastoris (pdb code 5hsa). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the Alcohol Oxidase AOX1 From Pichia Pastoris, PDB code: 5hsa:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Calcium binding site 1 out of 8 in 5hsa

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Calcium binding site 1 out of 8 in the Alcohol Oxidase AOX1 From Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Alcohol Oxidase AOX1 From Pichia Pastoris within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca705

b:64.3
occ:1.00
 O A:HOH1075 2.4 51.5 1.0
O A:GLU382 2.4 58.9 1.0
O A:HOH1109 2.4 50.8 1.0
O A:HOH1107 2.5 45.9 1.0
O A:HOH1005 2.5 66.9 1.0
C A:GLU382 3.6 57.1 1.0
CA A:GLU382 4.5 61.0 1.0
N A:TYR383 4.5 51.1 1.0
CA A:TYR383 4.6 46.9 1.0
OD2 A:ASP386 4.7 67.0 1.0
OD1 A:ASP386 4.7 68.0 1.0

Calcium binding site 2 out of 8 in 5hsa

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Calcium binding site 2 out of 8 in the Alcohol Oxidase AOX1 From Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Alcohol Oxidase AOX1 From Pichia Pastoris within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca706

b:76.6
occ:1.00
 O B:HOH1007 2.2 96.0 1.0
O B:HOH984 2.3 39.6 1.0
O B:GLU382 2.3 66.0 1.0
O B:HOH941 2.4 47.2 1.0
C B:GLU382 3.5 65.0 1.0
CA B:GLU382 4.4 65.0 1.0
O B:HOH919 4.4 60.9 1.0
N B:TYR383 4.5 62.7 1.0
OD1 B:ASP386 4.5 71.3 1.0
CA B:TYR383 4.6 59.5 1.0
OE1 B:GLU382 4.6 77.3 1.0
OD2 B:ASP386 4.6 71.6 1.0
CB B:GLU382 5.0 67.9 1.0
CG B:ASP386 5.0 70.4 1.0

Calcium binding site 3 out of 8 in 5hsa

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Calcium binding site 3 out of 8 in the Alcohol Oxidase AOX1 From Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Alcohol Oxidase AOX1 From Pichia Pastoris within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca707

b:76.9
occ:1.00
 O C:HOH1122 2.3 59.4 1.0
O C:GLU382 2.3 47.8 1.0
O C:HOH1066 2.4 52.1 1.0
O C:HOH980 2.5 67.3 1.0
O C:HOH1078 2.5 53.0 1.0
O C:HOH1095 2.5 55.0 1.0
C C:GLU382 3.6 48.7 1.0
OD1 C:ASP386 4.4 64.2 1.0
CA C:GLU382 4.5 52.0 1.0
N C:TYR383 4.5 46.0 1.0
OD2 C:ASP386 4.5 62.4 1.0
O C:HOH997 4.5 52.2 1.0
CA C:TYR383 4.5 43.1 1.0
CG C:GLU382 4.7 65.3 1.0
CG C:ASP386 4.9 62.1 1.0

Calcium binding site 4 out of 8 in 5hsa

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Calcium binding site 4 out of 8 in the Alcohol Oxidase AOX1 From Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Alcohol Oxidase AOX1 From Pichia Pastoris within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca708

b:97.6
occ:1.00
 O C:HOH1072 2.4 50.3 0.3
O C:HOH844 2.4 62.1 1.0
O C:ASP635 2.4 44.6 1.0
O C:HOH1101 2.5 55.9 1.0
O C:HOH1091 2.5 40.6 0.8
C2 C:GOL705 2.9 50.5 0.9
O1 C:GOL705 3.0 48.7 0.9
C3 C:GOL705 3.3 51.0 0.9
C1 C:GOL705 3.5 49.5 0.9
C C:ASP635 3.6 42.3 1.0
O3 C:GOL705 3.8 51.4 0.9
O2 C:GOL705 4.1 51.7 0.9
CA C:ASP635 4.1 42.2 1.0
N C:LEU636 4.7 41.0 1.0
CB C:ASP635 4.8 42.0 1.0
O C:GLU634 5.0 44.4 1.0
CA C:LEU636 5.0 39.8 1.0

Calcium binding site 5 out of 8 in 5hsa

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Calcium binding site 5 out of 8 in the Alcohol Oxidase AOX1 From Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Alcohol Oxidase AOX1 From Pichia Pastoris within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Ca704

b:97.6
occ:1.00
 O D:GLU382 2.3 80.0 1.0
O D:HOH962 2.3 62.7 1.0
O D:HOH906 2.3 57.2 1.0
O D:HOH807 2.4 71.7 1.0
C D:GLU382 3.5 80.3 1.0
OD1 D:ASP386 4.1 87.6 1.0
OD2 D:ASP386 4.3 87.9 1.0
N D:TYR383 4.4 79.1 1.0
CA D:TYR383 4.4 77.5 1.0
CA D:GLU382 4.5 81.4 1.0
NZ D:LYS387 4.6 80.9 1.0
CG D:ASP386 4.6 87.9 1.0

Calcium binding site 6 out of 8 in 5hsa

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Calcium binding site 6 out of 8 in the Alcohol Oxidase AOX1 From Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Alcohol Oxidase AOX1 From Pichia Pastoris within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Ca704

b:98.7
occ:1.00
 O E:GLU382 2.5 48.9 1.0
O E:HOH951 2.5 51.2 1.0
C E:GLU382 3.6 47.9 1.0
NZ E:LYS581 4.0 61.7 1.0
CA E:GLU382 4.5 52.5 1.0
CG E:GLU382 4.5 65.2 1.0
N E:TYR383 4.6 44.2 1.0
CA E:TYR383 4.6 40.6 1.0
OD1 E:ASP386 4.9 74.2 1.0
OD2 E:ASP386 5.0 71.8 1.0

Calcium binding site 7 out of 8 in 5hsa

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Calcium binding site 7 out of 8 in the Alcohol Oxidase AOX1 From Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Alcohol Oxidase AOX1 From Pichia Pastoris within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Ca706

b:89.9
occ:1.00
 O F:HOH1028 2.1 77.7 0.5
O F:HOH979 2.3 72.4 1.0
O F:HOH1089 2.3 45.7 1.0
O F:GLU382 2.3 53.9 1.0
C F:GLU382 3.6 53.7 1.0
OD2 F:ASP386 4.2 72.0 1.0
OD1 F:ASP386 4.3 71.0 1.0
N F:TYR383 4.5 50.9 1.0
CA F:TYR383 4.5 47.3 1.0
CA F:GLU382 4.5 56.6 1.0
O F:HOH946 4.5 48.2 1.0
CG F:ASP386 4.7 69.2 1.0

Calcium binding site 8 out of 8 in 5hsa

Go back to Calcium Binding Sites List in 5hsa
Calcium binding site 8 out of 8 in the Alcohol Oxidase AOX1 From Pichia Pastoris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Alcohol Oxidase AOX1 From Pichia Pastoris within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Ca704

b:0.4
occ:1.00
 O G:HOH953 2.4 87.0 0.5
O G:HOH988 2.4 81.0 0.5
O G:GLU382 2.4 65.1 1.0
C G:GLU382 3.6 64.6 1.0
CA G:TYR383 4.5 54.6 1.0
N G:TYR383 4.5 59.6 1.0
CA G:GLU382 4.5 68.5 1.0
OD2 G:ASP386 4.8 76.6 1.0
OD1 G:ASP386 4.8 76.5 1.0

Reference:

C.Koch, P.Neumann, O.Valerius, I.Feussner, R.Ficner. Crystal Structure of Alcohol Oxidase From Pichia Pastoris. Plos One V. 11 49846 2016.
ISSN: ESSN 1932-6203
PubMed: 26905908
DOI: 10.1371/JOURNAL.PONE.0149846
Page generated: Sun Jul 14 20:13:54 2024

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