Calcium in PDB 5kn1: Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form

The structure of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form, PDB code: 5kn1 was solved by K.M.Lewis, S.Byrd, C.Kang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.63 / 2.14
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	135.669, 165.604, 156.626, 90.00, 90.00, 90.00
R / Rfree (%)	18.4 / 20.1

The structure of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 30; Page 4, Binding sites: 31 - 40;

Binding sites:

The binding sites of Calcium atom in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form (pdb code 5kn1). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 40 binding sites of Calcium where determined in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form, PDB code: 5kn1:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Calcium binding site 1 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:51.8 occ:1.00 OD1 A:ASP13 2.2 53.4 1.0 O A:VAL15 2.3 49.6 1.0 OE2 A:GLU55 2.3 53.5 1.0 O A:HOH654 2.3 65.4 1.0 OE2 A:GLU59 2.5 51.1 1.0 CG A:ASP13 3.3 57.2 1.0 H A:VAL15 3.4 59.6 1.0 C A:VAL15 3.4 47.0 1.0 CD A:GLU55 3.5 64.2 1.0 HB A:VAL15 3.5 49.0 1.0 CD A:GLU59 3.7 50.9 1.0 OD2 A:ASP13 3.9 69.3 1.0 HA A:ASP13 3.9 64.8 1.0 HG2 A:GLU55 4.0 55.7 1.0 N A:VAL15 4.0 49.6 1.0 HA A:VAL16 4.0 67.8 1.0 CA A:VAL15 4.1 46.2 1.0 CG A:GLU55 4.2 46.4 1.0 HG3 A:GLU55 4.2 55.7 1.0 HG2 A:GLU59 4.2 55.0 1.0 HG3 A:GLU59 4.3 55.0 1.0 CB A:VAL15 4.3 40.8 1.0 CB A:ASP13 4.3 58.0 1.0 CG A:GLU59 4.3 45.9 1.0 HG12 A:VAL16 4.4 67.3 1.0 HB2 A:ASP13 4.4 69.6 1.0 OE1 A:GLU55 4.4 67.5 1.0 H A:ARG14 4.5 59.1 1.0 N A:VAL16 4.5 45.2 1.0 CA A:ASP13 4.5 54.0 1.0 OE1 A:GLU59 4.6 46.1 1.0 CA A:VAL16 4.8 56.5 1.0 HG12 A:VAL15 4.8 46.8 1.0 N A:ARG14 4.8 49.3 1.0 C A:ASP13 4.9 52.4 1.0 HG13 A:VAL16 4.9 67.3 1.0

Calcium binding site 2 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:35.7 occ:1.00 O A:HOH606 2.2 34.1 1.0 OE2 A:GLU105 2.3 39.8 1.0 OE2 A:GLU91 2.4 42.3 1.0 OE1 A:GLU240 2.4 33.5 1.0 OD2 A:ASP107 2.5 39.4 1.0 OE1 A:GLU91 2.5 43.6 1.0 O A:HOH559 2.6 40.7 1.0 CD A:GLU91 2.7 41.5 1.0 CD A:GLU105 3.0 47.8 1.0 OE1 A:GLU105 3.1 55.6 1.0 HB2 A:ASP107 3.3 45.6 1.0 CG A:ASP107 3.4 40.2 1.0 CD A:GLU240 3.4 40.6 1.0 HZ2 B:LYS85 3.5 84.5 1.0 HZ1 B:LYS85 3.6 84.5 1.0 HG A:SER94 3.7 55.2 1.0 CB A:ASP107 3.7 38.0 1.0 OE2 A:GLU240 3.8 38.9 1.0 HB3 A:ASP107 3.8 45.6 1.0 HA A:GLU240 3.9 41.3 1.0 OE1 A:GLU243 4.0 40.1 1.0 CA A:CA403 4.0 36.0 1.0 NZ B:LYS85 4.0 70.4 1.0 CG A:GLU91 4.2 46.7 1.0 OG A:SER94 4.2 46.0 1.0 HB2 A:GLU240 4.4 43.7 1.0 OD1 A:ASP107 4.5 38.2 1.0 CG A:GLU105 4.5 41.9 1.0 HG2 A:GLU91 4.5 56.0 1.0 HZ3 B:LYS85 4.6 84.5 1.0 O A:HOH643 4.6 50.0 1.0 CG A:GLU240 4.7 32.1 1.0 HG2 A:GLU105 4.7 50.2 1.0 CA A:GLU240 4.7 34.5 1.0 O A:HOH577 4.7 33.6 1.0 HE3 B:LYS85 4.8 0.9 1.0 HB3 A:GLU91 4.8 53.7 1.0 HG3 A:GLU91 4.8 56.0 1.0 CB A:GLU240 4.8 36.4 1.0 O A:HOH609 4.8 53.2 1.0 HG3 A:GLU105 4.9 50.2 1.0 O A:HOH709 4.9 59.3 1.0 O A:HOH652 4.9 56.3 1.0 HB2 A:GLU91 5.0 53.7 1.0 CB A:GLU91 5.0 44.7 1.0

Calcium binding site 3 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:36.0 occ:1.00 O A:HOH675 2.4 36.1 1.0 OD2 A:ASP107 2.4 39.4 1.0 OE2 A:GLU91 2.4 42.3 1.0 O A:HOH600 2.5 37.2 1.0 OE1 A:GLU243 2.5 40.1 1.0 OE2 A:GLU243 2.5 43.7 1.0 O A:HOH530 2.5 38.7 1.0 OD1 A:ASP107 2.6 38.2 1.0 CG A:ASP107 2.9 40.2 1.0 CD A:GLU243 2.9 45.4 1.0 CD A:GLU91 3.6 41.5 1.0 HG2 A:GLU91 3.6 56.0 1.0 HB3 A:TYR239 3.8 43.0 1.0 CA A:CA402 4.0 35.7 1.0 O A:HOH516 4.1 39.1 1.0 CG A:GLU91 4.1 46.7 1.0 HB2 A:TYR239 4.2 43.0 1.0 OG A:SER94 4.2 46.0 1.0 O A:HOH606 4.2 34.1 1.0 HG A:SER94 4.3 55.2 1.0 CB A:ASP107 4.3 38.0 1.0 O A:HOH652 4.4 56.3 1.0 HG3 A:GLU91 4.4 56.0 1.0 CG A:GLU243 4.4 38.6 1.0 CB A:TYR239 4.5 35.8 1.0 OE1 A:GLU91 4.6 43.6 1.0 HB2 A:ASP107 4.6 45.6 1.0 O A:HOH546 4.7 47.0 1.0 O A:GLU92 4.7 37.1 1.0 HG3 A:GLU243 4.8 46.3 1.0 HB3 A:ASP107 4.8 45.6 1.0 HG2 A:GLU243 4.8 46.3 1.0 OE2 A:GLU105 4.8 39.8 1.0 HB2 A:ASP93 4.9 55.7 1.0 HA A:ASP107 4.9 37.4 1.0

Calcium binding site 4 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca404 b:74.3 occ:1.00 O A:HOH551 2.3 38.6 1.0 O A:HOH608 2.5 32.8 1.0 O A:HOH686 2.6 51.8 0.5 O A:HOH579 2.6 40.8 1.0 OE1 A:GLU109 3.9 40.1 1.0 O A:HOH625 3.9 52.0 0.5 O A:HOH627 4.0 39.8 1.0 OE2 A:GLU109 4.1 45.0 1.0 O A:HOH691 4.3 16.9 1.0 OD2 A:ASP113 4.3 39.6 1.0 CD A:GLU109 4.5 37.6 1.0 O A:HOH590 4.5 44.5 1.0 HG1 A:THR114 5.0 46.9 1.0

Calcium binding site 5 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca405 b:58.4 occ:1.00 O A:HOH673 2.0 44.8 1.0 OE2 A:GLU117 2.6 46.1 1.0 OE1 A:GLU117 2.7 47.5 1.0 CD A:GLU117 3.0 48.6 1.0 O A:HOH623 4.4 44.8 1.0 CG A:GLU117 4.4 40.2 1.0 HG3 A:GLU117 4.6 48.2 1.0 HG2 A:GLU117 4.9 48.2 1.0 HB2 A:GLU117 5.0 35.5 1.0

Calcium binding site 6 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca406 b:63.3 occ:1.00 OD1 A:ASP121 2.5 32.6 1.0 O A:HOH549 2.5 40.8 1.0 CG A:ASP121 3.4 37.2 1.0 OD2 A:ASP121 3.6 36.6 1.0 HG3 A:GLU117 4.1 48.2 1.0 HA A:ASP121 4.5 40.1 1.0 HG2 A:GLU117 4.5 48.2 1.0 CG A:GLU117 4.7 40.2 1.0 CB A:ASP121 4.8 27.9 1.0 OE1 A:GLU117 4.8 47.5 1.0 O A:GLU117 4.9 32.0 1.0 CA A:ASP121 5.0 33.4 1.0

Calcium binding site 7 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca407 b:43.1 occ:1.00 O A:HOH525 2.3 44.5 1.0 OE1 A:GLU128 2.4 46.9 1.0 OE2 A:GLU128 3.1 43.7 1.0 CD A:GLU128 3.1 42.4 1.0 O A:HOH521 4.2 37.6 1.0 CG A:GLU128 4.5 40.4 1.0 O A:HOH665 4.7 41.9 1.0 HG2 A:GLU128 4.8 48.5 1.0 HG3 A:GLU128 4.9 48.5 1.0

Calcium binding site 8 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca408 b:38.2 occ:1.00 O A:HOH521 2.2 37.6 1.0 OE1 A:GLU135 2.2 42.1 1.0 CD A:GLU135 3.1 37.6 1.0 OE2 A:GLU135 3.2 39.1 1.0 CA A:CA409 3.9 59.6 1.0 O A:LEU129 4.3 35.7 1.0 HB2 A:LEU129 4.3 48.2 1.0 OE1 A:GLU128 4.4 46.9 1.0 CG A:GLU135 4.5 42.1 1.0 HG2 A:GLU135 4.6 50.5 1.0 O A:HOH629 4.7 49.4 1.0 O A:HOH665 4.7 41.9 1.0 O A:HOH630 4.9 51.1 1.0 HG3 A:GLU135 4.9 50.5 1.0

Calcium binding site 9 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 9 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca409 b:59.6 occ:1.00 O A:HOH630 2.4 51.1 1.0 OE1 A:GLU135 2.6 42.1 1.0 CD A:GLU135 3.3 37.6 1.0 HG3 A:GLU135 3.4 50.5 1.0 CG A:GLU135 3.8 42.1 1.0 CA A:CA408 3.9 38.2 1.0 HG2 A:GLU135 4.0 50.5 1.0 OE2 A:GLU135 4.2 39.1 1.0 O A:GLU131 4.7 44.1 1.0 O A:HOH572 4.8 38.6 1.0 HB3 A:GLU131 5.0 67.6 1.0

Calcium binding site 10 out of 40 in the Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 10 of Recombinant Bovine Skeletal Calsequestrin, High-CA2+ Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca410 b:46.6 occ:1.00 O A:HOH610 2.2 68.7 1.0 O A:HOH634 2.2 40.1 1.0 O A:HOH573 2.3 39.8 1.0 O A:THR189 2.4 46.4 1.0 O A:HOH672 2.5 55.2 1.0 OG1 A:THR189 2.6 44.4 1.0 O A:HOH514 2.6 39.1 1.0 HG1 A:THR189 3.1 53.3 1.0 C A:THR189 3.4 46.6 1.0 HB A:THR189 3.5 59.8 1.0 CB A:THR189 3.6 49.9 1.0 HA A:LEU190 4.0 43.0 1.0 OE1 A:GLU194 4.0 41.5 1.0 O A:HOH522 4.1 56.9 1.0 CA A:THR189 4.1 46.2 1.0 HG1 A:THR207 4.2 59.8 1.0 OD2 A:ASP196 4.3 43.9 1.0 OG1 A:THR207 4.4 49.8 1.0 N A:LEU190 4.4 41.5 1.0 HD23 A:LEU190 4.4 59.7 1.0 O A:LEU188 4.4 49.6 1.0 OD1 A:ASP196 4.7 45.3 1.0 CA A:LEU190 4.7 35.9 1.0 HA A:THR189 4.7 55.4 1.0 H A:LYS191 4.9 50.3 1.0 O A:HOH697 4.9 64.2 1.0 CG2 A:THR189 4.9 52.8 1.0 CG A:ASP196 4.9 47.1 1.0 HG21 A:THR189 4.9 63.4 1.0 HZ2 A:LYS191 5.0 77.8 1.0

K.M.Lewis, G.R.Munske, S.S.Byrd, J.Kang, H.J.Cho, E.Rios, C.Kang. Characterization of Post-Translational Modifications to Calsequestrins of Cardiac and Skeletal Muscle. Int J Mol Sci V. 17 2016.
ISSN: ESSN 1422-0067
PubMed: 27649144
DOI: 10.3390/IJMS17091539