Calcium in PDB 5kyi: Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature

Enzymatic activity of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature

All present enzymatic activity of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature:
3.1.31.1;

Protein crystallography data

The structure of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature, PDB code: 5kyi was solved by C.M.Kougentakis, J.L.Schlessman, B.Garcia-Moreno E., with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	38.36 / 1.70
*Space group*	P 1 2₁ 1
*Cell size a, b, c (Å), α, β, γ (°)*	31.129, 60.426, 38.446, 90.00, 93.86, 90.00
*R / R_free (%)*	17.5 / 20.3

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature (pdb code 5kyi). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature, PDB code: 5kyi:

Calcium binding site 1 out of 1 in 5kyi

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Calcium Binding Sites List in 5kyi

Calcium binding site 1 out of 1 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature

Mono view

Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Ca202 b:17.4 occ:1.00	OD1	A:ASP40	2.7	11.5	1.0
	OD2	A:ASP21	2.8	12.2	1.0
	O	A:HOH370	2.9	16.2	1.0
	O	A:HOH313	3.0	11.9	1.0
	OE2	A:GLU43	3.0	43.5	1.0
	O	A:THR41	3.1	12.4	1.0
	O5P	A:THP201	3.1	9.2	1.0
	O	A:HOH355	3.4	17.0	1.0
	CG	A:ASP21	3.6	12.1	1.0
	OD1	A:ASP21	3.7	11.0	1.0
	CG	A:ASP40	3.9	9.8	1.0
	O	A:HOH388	4.0	25.9	1.0
	CD	A:GLU43	4.0	39.7	1.0
	N	A:THR41	4.0	10.3	1.0
	NH2	A:ARG35	4.1	5.1	1.0
	OG1	A:THR41	4.1	13.3	1.0
	O	A:HOH341	4.1	11.6	1.0
	P2	A:THP201	4.1	8.2	1.0
	C	A:THR41	4.2	11.5	1.0
	O4P	A:THP201	4.2	8.0	1.0
	OD2	A:ASP40	4.6	14.9	1.0
	CA	A:THR41	4.6	10.7	1.0
	O	A:HOH308	4.7	26.9	1.0
	CZ	A:ARG35	4.7	5.3	1.0
	O6P	A:THP201	4.7	9.4	1.0
	CA	A:ASP40	4.7	7.8	1.0
	OE1	A:GLU43	4.8	38.5	1.0
	C	A:ASP40	4.8	8.2	1.0
	NE	A:ARG35	4.8	5.1	1.0
	CG	A:GLU43	4.9	33.2	1.0
	CB	A:ASP40	4.9	8.3	1.0
	CB	A:ASP21	5.0	10.5	1.0

Reference:

C.M.Kougentakis, J.L.Schlessman, B.Garcia-Moreno E.. Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs T62K/V66E pH 9 at Cryogenic Temperature To Be Published.

Page generated: Mon Jul 15 07:09:50 2024

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