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Calcium in PDB 5mnb: Cationic Trypsin in Complex with 2-Aminopyridine (Deuterated Sample at 295 K)

Enzymatic activity of Cationic Trypsin in Complex with 2-Aminopyridine (Deuterated Sample at 295 K)

All present enzymatic activity of Cationic Trypsin in Complex with 2-Aminopyridine (Deuterated Sample at 295 K):
3.4.21.4;

Protein crystallography data

The structure of Cationic Trypsin in Complex with 2-Aminopyridine (Deuterated Sample at 295 K), PDB code: 5mnb was solved by J.Schiebel, A.Heine, G.Klebe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 16.94 / 0.94
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 54.989, 58.537, 67.618, 90.00, 90.00, 90.00
R / Rfree (%) 9.6 / 10.7

Calcium Binding Sites:

The binding sites of Calcium atom in the Cationic Trypsin in Complex with 2-Aminopyridine (Deuterated Sample at 295 K) (pdb code 5mnb). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Cationic Trypsin in Complex with 2-Aminopyridine (Deuterated Sample at 295 K), PDB code: 5mnb:

Calcium binding site 1 out of 1 in 5mnb

Go back to Calcium Binding Sites List in 5mnb
Calcium binding site 1 out of 1 in the Cationic Trypsin in Complex with 2-Aminopyridine (Deuterated Sample at 295 K)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Cationic Trypsin in Complex with 2-Aminopyridine (Deuterated Sample at 295 K) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca301

b:8.2
occ:1.00
OE1 A:GLU70 2.3 9.8 1.0
O A:VAL75 2.3 9.7 1.0
OE2 A:GLU80 2.3 9.7 1.0
O A:ASN72 2.3 8.8 1.0
O A:HOH469 2.4 11.4 1.0
O A:HOH405 2.4 9.7 1.0
H1 A:HOH405 2.6 11.7 1.0
H2 A:HOH405 3.1 11.7 1.0
CD A:GLU70 3.3 8.5 1.0
HA A:VAL76 3.4 12.5 1.0
CD A:GLU80 3.4 9.6 1.0
C A:VAL75 3.4 8.9 1.0
C A:ASN72 3.5 7.6 1.0
H A:GLU77 3.5 12.3 1.0
H A:VAL75 3.6 10.7 1.0
HG3 A:GLU77 3.7 14.4 1.0
CG A:GLU80 3.8 12.3 1.0
OE2 A:GLU70 3.8 10.6 1.0
H A:ASP71 3.9 9.7 1.0
HA A:GLU70 3.9 9.4 1.0
CA A:VAL76 4.1 10.4 1.0
N A:GLU77 4.2 10.2 1.0
N A:VAL76 4.2 10.2 1.0
HB3 A:ASN72 4.2 11.0 1.0
HB2 A:GLU77 4.3 13.9 1.0
N A:VAL75 4.3 8.9 1.0
H A:ASN72 4.3 9.6 1.0
OE1 A:GLU77 4.3 11.5 1.0
CA A:ILE73 4.3 8.0 1.0
N A:ILE73 4.3 7.9 1.0
N A:ASN72 4.4 8.0 1.0
CA A:VAL75 4.5 9.3 1.0
CA A:ASN72 4.5 8.1 1.0
HB3 A:GLU70 4.5 9.6 1.0
O A:HOH466 4.5 12.6 1.0
CG A:GLU77 4.5 12.0 1.0
OE1 A:GLU80 4.5 10.8 1.0
C A:ILE73 4.6 8.1 1.0
N A:ASP71 4.6 8.1 1.0
CG A:GLU70 4.6 8.6 1.0
HB A:VAL75 4.6 12.8 1.0
C A:VAL76 4.7 10.8 1.0
CA A:GLU70 4.7 7.9 1.0
CB A:GLU77 4.8 11.6 1.0
CB A:GLU70 4.8 8.0 1.0
CB A:ASN72 4.9 9.2 1.0
CD A:GLU77 4.9 12.9 1.0
O A:HOH558 4.9 26.8 1.0
N A:ASN74 4.9 8.0 1.0
H A:ASN74 4.9 9.6 0.8
H A:ASN74 4.9 9.6 0.2
O A:ILE73 5.0 9.7 1.0
HG2 A:GLU70 5.0 10.3 1.0

Reference:

J.Schiebel, R.Gaspari, A.Sandner, K.Ngo, H.D.Gerber, A.Cavalli, A.Ostermann, A.Heine, G.Klebe. Charges Shift Protonation: Neutron Diffraction Reveals That Aniline and 2-Aminopyridine Become Protonated Upon Binding to Trypsin. Angew. Chem. Int. Ed. Engl. V. 56 4887 2017.
ISSN: ESSN 1521-3773
PubMed: 28371253
DOI: 10.1002/ANIE.201701038
Page generated: Mon Jul 15 08:29:12 2024

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