Calcium in PDB 5t2h: Engineered Variant of I-Onui Meganuclease Targeting the Human Tcra Gene; Harbors 43 Point Mutations Relative to Wild-Type I-Onui

The structure of Engineered Variant of I-Onui Meganuclease Targeting the Human Tcra Gene; Harbors 43 Point Mutations Relative to Wild-Type I-Onui, PDB code: 5t2h was solved by B.L.Stoddard, R.Werther, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.77 / 2.52
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	39.696, 68.928, 157.530, 90.00, 90.00, 90.00
R / Rfree (%)	21.2 / 26.5

The binding sites of Calcium atom in the Engineered Variant of I-Onui Meganuclease Targeting the Human Tcra Gene; Harbors 43 Point Mutations Relative to Wild-Type I-Onui (pdb code 5t2h). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Engineered Variant of I-Onui Meganuclease Targeting the Human Tcra Gene; Harbors 43 Point Mutations Relative to Wild-Type I-Onui, PDB code: 5t2h:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Engineered Variant of I-Onui Meganuclease Targeting the Human Tcra Gene; Harbors 43 Point Mutations Relative to Wild-Type I-Onui


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Engineered Variant of I-Onui Meganuclease Targeting the Human Tcra Gene; Harbors 43 Point Mutations Relative to Wild-Type I-Onui within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:41.2 occ:1.00 OE2 A:GLU178 2.3 47.6 1.0 OP1 B:DC14 2.4 37.7 1.0 O A:ALA21 2.4 43.0 1.0 O A:HOH504 2.5 37.1 1.0 OP2 C:DA16 2.6 43.3 1.0 C A:ALA21 3.4 40.6 1.0 P C:DA16 3.5 47.8 1.0 C5' C:DA16 3.5 46.3 1.0 CD A:GLU178 3.5 48.4 1.0 P B:DC14 3.7 40.9 1.0 OP1 C:DA16 3.7 50.4 1.0 CA A:ALA21 3.9 43.6 1.0 O5' C:DA16 3.9 42.1 1.0 O B:HOH101 4.0 38.9 1.0 O3' B:DT13 4.0 46.6 1.0 NE2 A:GLN204 4.0 49.1 1.0 OE1 A:GLU178 4.3 60.0 1.0 CG A:GLU178 4.3 39.9 1.0 OP2 B:DC14 4.5 47.0 1.0 O A:ASP20 4.5 42.6 1.0 C4' C:DA16 4.5 40.5 1.0 N A:GLU22 4.6 36.2 1.0 CB A:ALA21 4.7 40.1 1.0 C4' B:DC14 4.8 35.6 1.0 O5' B:DC14 4.8 48.0 1.0 C5' B:DC14 4.9 39.8 1.0 CD A:GLN204 4.9 44.3 1.0 O3' C:DT15 4.9 50.6 1.0 OE1 A:GLU22 5.0 49.9 1.0

Calcium binding site 2 out of 2 in the Engineered Variant of I-Onui Meganuclease Targeting the Human Tcra Gene; Harbors 43 Point Mutations Relative to Wild-Type I-Onui


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Engineered Variant of I-Onui Meganuclease Targeting the Human Tcra Gene; Harbors 43 Point Mutations Relative to Wild-Type I-Onui within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:43.8 occ:1.00 OP1 C:DT15 2.3 39.8 1.0 O A:GLY177 2.3 46.1 1.0 OP2 B:DA15 2.4 37.5 1.0 OE2 A:GLU22 2.5 38.4 1.0 O A:HOH515 2.5 37.4 1.0 O A:HOH519 2.7 48.2 1.0 C A:GLY177 3.4 41.9 1.0 CD A:GLU22 3.5 46.8 1.0 O B:HOH101 3.5 38.9 1.0 P C:DT15 3.8 38.7 1.0 P B:DA15 3.8 39.8 1.0 CA A:GLY177 3.9 37.3 1.0 OE1 A:GLU22 3.9 49.9 1.0 O3' C:DA14 4.3 41.1 1.0 C5' C:DT15 4.3 46.1 1.0 C5' B:DA15 4.3 42.2 1.0 O5' B:DA15 4.4 35.5 1.0 OP1 B:DA15 4.4 41.7 1.0 O5' C:DT15 4.5 49.7 1.0 C4' C:DT15 4.6 48.3 1.0 N A:GLU178 4.6 38.8 1.0 CG A:GLU22 4.6 41.1 1.0 O A:ALA176 4.7 46.3 1.0 OP2 C:DT15 4.8 47.3 1.0 O3' B:DC14 4.9 34.8 1.0 CA A:GLU178 4.9 38.5 1.0 CD2 A:LEU49 5.0 42.8 1.0

B.L.Stoddard, B.L.Stoddard, R.Werther. N/A N/A.