Calcium in PDB 5t2o: Engineered Variant of I-Onui Meganuclease Targeting the Anopheles AGAP011377 Gene; Harbors 53 Point Mutations Relative to Wild-Type I- Onui

The structure of Engineered Variant of I-Onui Meganuclease Targeting the Anopheles AGAP011377 Gene; Harbors 53 Point Mutations Relative to Wild-Type I- Onui, PDB code: 5t2o was solved by B.L.Stoddard, R.Werther, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.24 / 2.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.180, 64.835, 167.005, 90.00, 90.00, 90.00
R / Rfree (%)	22.4 / 28.1

The binding sites of Calcium atom in the Engineered Variant of I-Onui Meganuclease Targeting the Anopheles AGAP011377 Gene; Harbors 53 Point Mutations Relative to Wild-Type I- Onui (pdb code 5t2o). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Engineered Variant of I-Onui Meganuclease Targeting the Anopheles AGAP011377 Gene; Harbors 53 Point Mutations Relative to Wild-Type I- Onui, PDB code: 5t2o:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Engineered Variant of I-Onui Meganuclease Targeting the Anopheles AGAP011377 Gene; Harbors 53 Point Mutations Relative to Wild-Type I- Onui


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Engineered Variant of I-Onui Meganuclease Targeting the Anopheles AGAP011377 Gene; Harbors 53 Point Mutations Relative to Wild-Type I- Onui within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:34.2 occ:1.00 OP1 B:DC14 2.4 32.6 1.0 O A:ALA21 2.4 32.1 1.0 OE2 A:GLU178 2.5 36.0 1.0 OP2 C:DT16 2.5 34.6 1.0 O C:HOH103 3.0 31.4 1.0 C A:ALA21 3.5 34.3 1.0 CD A:GLU178 3.5 33.9 1.0 P C:DT16 3.6 37.8 1.0 P B:DC14 3.7 34.9 1.0 OE1 A:GLU178 3.7 34.2 1.0 C5' C:DT16 3.7 32.4 1.0 O C:HOH101 3.9 29.8 1.0 CA A:ALA21 3.9 31.6 1.0 O3' B:DT13 4.0 37.3 1.0 O5' C:DT16 4.1 33.6 1.0 OP1 C:DT16 4.2 35.2 1.0 O A:ASP20 4.2 33.2 1.0 NE2 A:GLN204 4.4 31.7 1.0 C4' C:DT16 4.5 32.7 1.0 OE1 A:GLN204 4.5 30.5 1.0 OP2 B:DC14 4.5 38.2 1.0 OE1 A:GLU22 4.5 34.3 1.0 N A:GLU22 4.6 33.9 1.0 CE A:LYS103 4.7 37.4 1.0 C4' B:DC14 4.8 34.7 1.0 CG A:GLU178 4.8 32.6 1.0 O5' B:DC14 4.8 34.7 1.0 CB A:ALA21 4.9 34.5 1.0 CD A:GLN204 4.9 33.2 1.0 C5' B:DC14 4.9 35.2 1.0 N A:ALA21 5.0 34.1 1.0 O3' C:DA15 5.0 33.0 1.0

Calcium binding site 2 out of 2 in the Engineered Variant of I-Onui Meganuclease Targeting the Anopheles AGAP011377 Gene; Harbors 53 Point Mutations Relative to Wild-Type I- Onui


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Engineered Variant of I-Onui Meganuclease Targeting the Anopheles AGAP011377 Gene; Harbors 53 Point Mutations Relative to Wild-Type I- Onui within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:42.8 occ:1.00 OP1 C:DA15 2.4 31.1 1.0 O A:GLY177 2.4 36.9 1.0 OE2 A:GLU22 2.5 37.9 1.0 OP2 B:DT15 2.9 35.1 1.0 C A:GLY177 3.2 36.9 1.0 CA A:GLY177 3.3 34.5 1.0 CD A:GLU22 3.6 35.1 1.0 O A:SER176 3.9 40.5 1.0 P C:DA15 3.9 30.7 1.0 NZ A:LYS262 4.2 36.6 1.0 P B:DT15 4.2 33.5 1.0 O C:HOH101 4.3 29.8 1.0 C5' B:DT15 4.4 36.5 1.0 N A:GLY177 4.4 37.4 1.0 OE1 A:GLU22 4.4 34.3 1.0 CG A:GLU22 4.5 35.7 1.0 N A:GLU178 4.5 36.1 1.0 C A:SER176 4.5 38.0 1.0 C5' C:DA15 4.6 32.5 1.0 O5' B:DT15 4.6 34.5 1.0 O3' C:DA14 4.6 31.7 1.0 OP2 C:DA15 4.7 37.3 1.0 O5' C:DA15 4.8 32.3 1.0 CD2 A:LEU49 4.9 37.1 1.0 C4' C:DA15 5.0 33.1 1.0

B.L.Stoddard, R.Werther. Tbd To Be Published.