Calcium in PDB 5tua: Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1

The structure of Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1, PDB code: 5tua was solved by J.Guo, W.Zeng, Y.Jiang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.59 / 3.30
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	88.535, 156.371, 217.128, 90.00, 90.00, 90.00
R / Rfree (%)	31 / 32.3

The structure of Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1 also contains other interesting chemical elements:

Barium	(Ba)	9 atoms
Sodium	(Na)	1 atom

The binding sites of Calcium atom in the Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1 (pdb code 5tua). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1, PDB code: 5tua:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca801 b:0.8 occ:1.00 O A:GLU341 2.3 0.9 1.0 OD1 A:ASP337 2.3 0.1 1.0 OD1 A:ASN339 2.4 0.2 1.0 OD1 A:ASP335 2.4 0.2 1.0 OD2 A:ASP337 2.5 1.0 1.0 CG A:ASP337 2.7 0.5 1.0 CG A:ASN339 3.1 0.9 1.0 ND2 A:ASN339 3.1 0.3 1.0 C A:GLU341 3.4 99.6 1.0 CG A:ASP335 3.7 99.8 1.0 OD1 A:ASP343 3.9 0.9 1.0 N A:GLU341 4.1 95.8 1.0 CB A:ASP337 4.2 0.2 1.0 N A:ILE342 4.2 93.9 1.0 CA A:GLU341 4.3 96.5 1.0 CA A:ILE342 4.4 97.1 1.0 OD2 A:ASP335 4.4 98.3 1.0 CB A:ASN339 4.6 0.6 1.0 OE1 A:GLN346 4.6 0.4 1.0 CB A:ASP335 4.6 97.8 1.0 CB A:GLU341 4.7 98.8 1.0 N A:ASP337 4.7 0.4 1.0 CA A:ASP335 4.7 0.5 1.0 N A:ASN339 4.9 0.4 1.0 CA A:ASP337 4.9 0.5 1.0 CG A:ASP343 5.0 0.7 1.0

Calcium binding site 2 out of 2 in the Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of A Na+-Selective Mutant of Two-Pore Channel From Arabidopsis Thaliana ATTPC1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca802 b:79.6 occ:1.00 OD2 A:ASP170 2.4 88.4 1.0 CZ2 A:TRP92 2.5 84.9 1.0 CB A:ASP170 2.5 91.0 1.0 OE2 A:GLU124 2.6 81.3 1.0 CG A:ASP170 2.6 89.9 1.0 OD1 A:ASP166 2.8 80.1 1.0 CH2 A:TRP92 3.1 83.1 1.0 OE1 A:GLU124 3.2 85.8 1.0 CD A:GLU124 3.2 83.2 1.0 OD1 A:ASP170 3.6 90.5 1.0 CE2 A:TRP92 3.7 83.5 1.0 CG A:ASP166 4.0 79.7 1.0 CA A:ASP170 4.0 94.3 1.0 NH2 A:ARG191 4.0 65.2 1.0 NE1 A:TRP92 4.3 84.9 1.0 OD2 A:ASP166 4.5 78.3 1.0 CZ3 A:TRP92 4.5 79.9 1.0 O A:ASP166 4.6 88.4 1.0 N A:ASP170 4.6 95.2 1.0 CG A:GLU124 4.7 82.6 1.0 CB A:ALA187 4.7 65.0 1.0 OG A:SER120 4.7 85.9 1.0 C A:ASP166 4.9 87.2 1.0 CD2 A:TRP92 5.0 80.4 1.0

J.Guo, W.Zeng, Y.Jiang. Tuning the Ion Selectivity of Two-Pore Channels. Proc. Natl. Acad. Sci. V. 114 1009 2017U.S.A..
ISSN: ESSN 1091-6490
PubMed: 28096396
DOI: 10.1073/PNAS.1616191114