Calcium in PDB 5tx6: Structure of Tgf-BETA2 Derivative with Deletion of Residues 52-71 and 10 Single Amino Acid Mutations (Mmtgf-BETA2-7M)

The structure of Structure of Tgf-BETA2 Derivative with Deletion of Residues 52-71 and 10 Single Amino Acid Mutations (Mmtgf-BETA2-7M), PDB code: 5tx6 was solved by E.M.Petrunak, A.P.Hinck, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.48 / 2.75
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	81.744, 81.744, 80.926, 90.00, 90.00, 120.00
R / Rfree (%)	21.3 / 27.2

The binding sites of Calcium atom in the Structure of Tgf-BETA2 Derivative with Deletion of Residues 52-71 and 10 Single Amino Acid Mutations (Mmtgf-BETA2-7M) (pdb code 5tx6). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Structure of Tgf-BETA2 Derivative with Deletion of Residues 52-71 and 10 Single Amino Acid Mutations (Mmtgf-BETA2-7M), PDB code: 5tx6:

Calcium binding site 1 out of 1 in the Structure of Tgf-BETA2 Derivative with Deletion of Residues 52-71 and 10 Single Amino Acid Mutations (Mmtgf-BETA2-7M)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Tgf-BETA2 Derivative with Deletion of Residues 52-71 and 10 Single Amino Acid Mutations (Mmtgf-BETA2-7M) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca201 b:27.4 occ:1.00 O A:GLN100 2.4 22.6 1.0 OE1 A:GLU99 2.4 29.2 1.0 OE2 A:GLU99 2.7 28.8 1.0 CD A:GLU99 2.9 21.9 1.0 C A:GLN100 3.5 24.6 1.0 H A:GLN100 3.9 26.6 1.0 HA A:LEU101 4.0 28.0 1.0 N A:GLN100 4.2 22.2 1.0 HB2 A:GLN100 4.3 20.6 1.0 CA A:GLN100 4.3 17.5 1.0 HD23 A:LEU101 4.4 22.6 1.0 HB3 A:GLN100 4.4 20.6 1.0 CG A:GLU99 4.4 22.8 1.0 N A:LEU101 4.5 27.8 1.0 CB A:GLN100 4.6 17.2 1.0 CA A:LEU101 4.7 23.4 1.0 H A:SER102 4.8 28.8 1.0 HG3 A:GLU99 4.8 27.4 1.0 HG2 A:GLU99 4.9 27.4 1.0 HB3 A:GLU99 4.9 29.6 1.0

S.K.Kim, L.Barron, C.S.Hinck, E.M.Petrunak, K.E.Cano, A.Thangirala, B.Iskra, M.Brothers, M.Vonberg, B.Leal, B.Richter, R.Kodali, A.B.Taylor, S.Du, C.O.Barnes, T.Sulea, G.Calero, P.J.Hart, M.J.Hart, B.Demeler, A.P.Hinck. An Engineered Transforming Growth Factor Beta (Tgf-Beta ) Monomer That Functions As A Dominant Negative to Block Tgf-Beta Signaling. J. Biol. Chem. V. 292 7173 2017.
ISSN: ESSN 1083-351X
PubMed: 28228478
DOI: 10.1074/JBC.M116.768754