Calcium in PDB 5uvl: Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation

All present enzymatic activity of Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation:
3.4.21.64;

The structure of Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation, PDB code: 5uvl was solved by J.M.Martin-Garcia, C.E.Conrad, G.Nelson, N.Stander, N.A.Zatsepin, J.Zook, L.Zhu, J.Geiger, E.Chun, D.Kissick, M.C.Hilgart, C.Ogata, A.Ishchenko, N.Nagaratnam, S.Roy-Chowdhury, J.Coe, G.Subramanian, A.Schaffer, D.James, G.Ketawala, N.Venugopalan, S.Xu, S.Corcoran, D.Ferguson, U.Weierstall, J.C.H.Spence, V.Cherezov, P.Fromme, R.F.Fischetti, W.Liu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.01 / 2.65
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	68.300, 68.300, 108.200, 90.00, 90.00, 90.00
R / Rfree (%)	22.5 / 24.3

The binding sites of Calcium atom in the Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation (pdb code 5uvl). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation, PDB code: 5uvl:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1003 b:55.1 occ:1.00 O A:PRO175 2.4 60.1 1.0 O A:VAL177 2.5 70.2 1.0 OD2 A:ASP200 2.5 88.0 1.0 OD1 A:ASP200 2.6 80.0 1.0 CG A:ASP200 2.9 77.0 1.0 C A:PRO175 3.5 52.1 1.0 C A:VAL177 3.7 61.5 1.0 O A:VAL198 4.2 58.0 1.0 N A:VAL177 4.2 59.2 1.0 CA A:PRO175 4.2 54.6 1.0 CB A:ASP200 4.4 68.2 1.0 N A:SER176 4.5 49.7 1.0 C A:SER176 4.5 61.3 1.0 O A:GLU174 4.6 62.3 1.0 CA A:CYS178 4.6 61.6 1.0 N A:CYS178 4.6 62.0 1.0 CA A:VAL177 4.6 58.3 1.0 N A:THR179 4.6 56.3 1.0 CA A:SER176 4.7 57.0 1.0 OG1 A:THR179 4.9 58.5 1.0

Calcium binding site 2 out of 2 in the Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-Crystals Using Synchrotron Radiation within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1004 b:51.9 occ:1.00 O A:THR16 2.4 77.5 1.0 OD2 A:ASP260 2.4 69.2 1.0 OD1 A:ASP260 2.6 73.0 1.0 CG A:ASP260 2.8 71.0 1.0 C A:THR16 3.6 69.0 1.0 CB A:ASP260 4.3 67.4 1.0 CB A:THR16 4.4 65.3 1.0 CA A:THR16 4.4 62.5 1.0 N A:SER17 4.5 69.1 1.0 CA A:SER17 4.5 71.5 1.0 OD1 A:ASN257 4.6 72.4 1.0 NH2 A:ARG12 4.8 54.0 1.0 OG1 A:THR16 4.9 66.7 1.0 CG A:ASN257 4.9 70.2 1.0

J.M.Martin-Garcia, C.E.Conrad, G.Nelson, N.Stander, N.A.Zatsepin, J.Zook, L.Zhu, J.Geiger, E.Chun, D.Kissick, M.C.Hilgart, C.Ogata, A.Ishchenko, N.Nagaratnam, S.Roy-Chowdhury, J.Coe, G.Subramanian, A.Schaffer, D.James, G.Ketwala, N.Venugopalan, S.Xu, S.Corcoran, D.Ferguson, U.Weierstall, J.C.H.Spence, V.Cherezov, P.Fromme, R.F.Fischetti, W.Liu. Serial Millisecond Crystallography of Membrane and Soluble Protein Microcrystals Using Synchrotron Radiation. Iucrj V. 4 439 2017.
ISSN: ESSN 2052-2525
PubMed: 28875031
DOI: 10.1107/S205225251700570X