Calcium in PDB 5w7e: Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
Enzymatic activity of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
All present enzymatic activity of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine:
3.1.1.77;
Protein crystallography data
The structure of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine, PDB code: 5w7e
was solved by
A.Gorelik,
K.Illes,
B.Nagar,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
40.07 /
1.83
|
|
Space group
|
P 1 21 1
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
81.425,
85.373,
93.398,
90.00,
103.64,
90.00
|
|
R / Rfree (%)
|
15.7 /
19.8
|
Calcium Binding Sites:
The binding sites of Calcium atom in the Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
(pdb code 5w7e). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 6 binding sites of Calcium where determined in the
Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine, PDB code: 5w7e:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
Calcium binding site 1 out
of 6 in 5w7e
Go back to
Calcium Binding Sites List in 5w7e
Calcium binding site 1 out
of 6 in the Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca601
b:17.6
occ:1.00
|
OD2
|
A:ASP183
|
2.2
|
18.1
|
1.0
|
|
OD1
|
A:ASP207
|
2.3
|
24.0
|
1.0
|
|
OD1
|
A:ASP187
|
2.3
|
17.2
|
1.0
|
|
OD2
|
A:ASP204
|
2.3
|
19.0
|
1.0
|
|
O
|
A:HIS189
|
2.3
|
15.1
|
1.0
|
|
OD1
|
A:ASP185
|
2.3
|
20.4
|
1.0
|
|
CG
|
A:ASP187
|
3.2
|
19.2
|
1.0
|
|
H
|
A:ASP187
|
3.2
|
24.6
|
1.0
|
|
CG
|
A:ASP185
|
3.3
|
24.7
|
1.0
|
|
H
|
A:HIS189
|
3.3
|
16.7
|
1.0
|
|
HA
|
A:ASP207
|
3.4
|
27.0
|
1.0
|
|
CG
|
A:ASP204
|
3.5
|
21.1
|
1.0
|
|
CG
|
A:ASP183
|
3.5
|
17.6
|
1.0
|
|
CG
|
A:ASP207
|
3.5
|
22.2
|
1.0
|
|
C
|
A:HIS189
|
3.5
|
15.5
|
1.0
|
|
OD2
|
A:ASP187
|
3.6
|
19.7
|
1.0
|
|
OD2
|
A:ASP185
|
3.6
|
25.0
|
1.0
|
|
HB2
|
A:ASP204
|
3.6
|
20.8
|
1.0
|
|
HA
|
A:ASP183
|
3.7
|
22.2
|
1.0
|
|
H
|
A:ASP185
|
3.7
|
28.6
|
1.0
|
|
HB2
|
A:HIS189
|
3.9
|
16.6
|
1.0
|
|
CB
|
A:ASP204
|
4.0
|
17.4
|
1.0
|
|
N
|
A:ASP187
|
4.0
|
20.5
|
1.0
|
|
N
|
A:HIS189
|
4.0
|
13.9
|
1.0
|
|
CA
|
A:CA602
|
4.1
|
20.1
|
1.0
|
|
H
|
A:SER186
|
4.1
|
34.1
|
1.0
|
|
HB3
|
A:ASP204
|
4.1
|
20.8
|
1.0
|
|
HA
|
A:SER190
|
4.1
|
18.2
|
1.0
|
|
CA
|
A:ASP207
|
4.2
|
22.5
|
1.0
|
|
CA
|
A:HIS189
|
4.2
|
13.2
|
1.0
|
|
OD1
|
A:ASP183
|
4.3
|
16.7
|
1.0
|
|
CB
|
A:ASP207
|
4.3
|
21.4
|
1.0
|
|
CA
|
A:ASP183
|
4.3
|
18.5
|
1.0
|
|
H
|
A:LYS188
|
4.3
|
23.3
|
0.5
|
|
H
|
A:LYS188
|
4.3
|
23.3
|
0.5
|
|
CB
|
A:ASP187
|
4.4
|
18.4
|
1.0
|
|
N
|
A:ASP185
|
4.4
|
23.9
|
1.0
|
|
OD2
|
A:ASP207
|
4.4
|
19.9
|
1.0
|
|
N
|
A:SER186
|
4.4
|
28.4
|
1.0
|
|
CB
|
A:ASP183
|
4.4
|
16.9
|
1.0
|
|
HB2
|
A:ASP207
|
4.5
|
25.7
|
1.0
|
|
OD1
|
A:ASP204
|
4.5
|
20.0
|
1.0
|
|
CB
|
A:HIS189
|
4.5
|
13.8
|
1.0
|
|
H
|
A:VAL184
|
4.5
|
25.2
|
1.0
|
|
CA
|
A:ASP187
|
4.6
|
17.5
|
1.0
|
|
N
|
A:SER190
|
4.6
|
14.2
|
1.0
|
|
CB
|
A:ASP185
|
4.6
|
25.5
|
1.0
|
|
C
|
A:ASP183
|
4.6
|
21.9
|
1.0
|
|
N
|
A:LYS188
|
4.6
|
19.4
|
1.0
|
|
HB2
|
A:ASP183
|
4.7
|
20.3
|
1.0
|
|
C
|
A:ASP185
|
4.7
|
25.9
|
1.0
|
|
N
|
A:VAL184
|
4.7
|
21.0
|
1.0
|
|
C
|
A:ASP187
|
4.7
|
16.7
|
1.0
|
|
O
|
A:ASP207
|
4.7
|
24.6
|
1.0
|
|
HB3
|
A:ASP187
|
4.8
|
22.1
|
1.0
|
|
CA
|
A:ASP185
|
4.8
|
25.2
|
1.0
|
|
CA
|
A:SER190
|
4.8
|
15.1
|
1.0
|
|
C
|
A:ASP207
|
4.9
|
25.5
|
1.0
|
|
HB3
|
A:HIS189
|
4.9
|
16.6
|
1.0
|
|
HB3
|
A:ASP185
|
5.0
|
30.6
|
1.0
|
|
Calcium binding site 2 out
of 6 in 5w7e
Go back to
Calcium Binding Sites List in 5w7e
Calcium binding site 2 out
of 6 in the Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca602
b:20.1
occ:1.00
|
OD2
|
A:ASP187
|
2.2
|
19.7
|
1.0
|
|
O
|
A:VAL212
|
2.3
|
21.5
|
1.0
|
|
O
|
A:ASN206
|
2.3
|
23.9
|
1.0
|
|
OD2
|
A:ASP185
|
2.3
|
25.0
|
1.0
|
|
O
|
A:ASP209
|
2.5
|
24.7
|
1.0
|
|
OD2
|
A:ASP204
|
2.5
|
19.0
|
1.0
|
|
OD1
|
A:ASP204
|
2.6
|
20.0
|
1.0
|
|
CG
|
A:ASP204
|
2.9
|
21.1
|
1.0
|
|
HA
|
A:ASP207
|
3.3
|
27.0
|
1.0
|
|
CG
|
A:ASP187
|
3.4
|
19.2
|
1.0
|
|
C
|
A:ASN206
|
3.4
|
23.8
|
1.0
|
|
CG
|
A:ASP185
|
3.4
|
24.7
|
1.0
|
|
C
|
A:VAL212
|
3.5
|
22.0
|
1.0
|
|
H
|
A:ASP209
|
3.5
|
36.9
|
1.0
|
|
HB
|
A:VAL212
|
3.5
|
30.5
|
1.0
|
|
C
|
A:ASP209
|
3.5
|
27.3
|
1.0
|
|
H
|
A:VAL212
|
3.7
|
29.7
|
1.0
|
|
HB3
|
A:TYR213
|
3.7
|
21.5
|
1.0
|
|
HA
|
A:LYS210
|
3.7
|
32.9
|
1.0
|
|
OD1
|
A:ASP185
|
3.8
|
20.4
|
1.0
|
|
H
|
A:ASN206
|
3.8
|
28.7
|
1.0
|
|
OD1
|
A:ASP187
|
3.9
|
17.2
|
1.0
|
|
CA
|
A:ASP207
|
4.0
|
22.5
|
1.0
|
|
CA
|
A:CA601
|
4.1
|
17.6
|
1.0
|
|
HB3
|
A:ASN206
|
4.1
|
32.5
|
1.0
|
|
N
|
A:ASP207
|
4.1
|
23.2
|
1.0
|
|
HG12
|
A:VAL212
|
4.2
|
29.6
|
1.0
|
|
CA
|
A:VAL212
|
4.2
|
24.2
|
1.0
|
|
CB
|
A:VAL212
|
4.2
|
25.4
|
1.0
|
|
N
|
A:ASP209
|
4.2
|
30.8
|
1.0
|
|
HB3
|
A:ASP209
|
4.2
|
34.7
|
1.0
|
|
N
|
A:VAL212
|
4.3
|
24.8
|
1.0
|
|
N
|
A:LYS210
|
4.4
|
26.0
|
1.0
|
|
CB
|
A:ASP204
|
4.4
|
17.4
|
1.0
|
|
HD2
|
A:TYR213
|
4.4
|
24.2
|
1.0
|
|
CA
|
A:ASN206
|
4.4
|
25.5
|
1.0
|
|
CA
|
A:LYS210
|
4.4
|
27.4
|
1.0
|
|
CA
|
A:ASP209
|
4.4
|
28.5
|
1.0
|
|
HA
|
A:TYR213
|
4.4
|
22.5
|
1.0
|
|
HB3
|
A:ASP187
|
4.5
|
22.1
|
1.0
|
|
N
|
A:ASN206
|
4.5
|
23.9
|
1.0
|
|
N
|
A:TYR213
|
4.5
|
20.8
|
1.0
|
|
CB
|
A:TYR213
|
4.6
|
17.9
|
1.0
|
|
CB
|
A:ASP187
|
4.6
|
18.4
|
1.0
|
|
C
|
A:ASP207
|
4.6
|
25.5
|
1.0
|
|
CB
|
A:ASP185
|
4.7
|
25.5
|
1.0
|
|
CG1
|
A:VAL212
|
4.7
|
24.6
|
1.0
|
|
HB2
|
A:ASP204
|
4.7
|
20.8
|
1.0
|
|
CA
|
A:TYR213
|
4.7
|
18.8
|
1.0
|
|
CB
|
A:ASN206
|
4.7
|
27.1
|
1.0
|
|
HB3
|
A:ASP204
|
4.8
|
20.8
|
1.0
|
|
HB2
|
A:ASP185
|
4.8
|
30.6
|
1.0
|
|
C
|
A:LYS210
|
4.8
|
24.6
|
1.0
|
|
HB3
|
A:ASP185
|
4.8
|
30.6
|
1.0
|
|
CB
|
A:ASP209
|
4.9
|
28.9
|
1.0
|
|
H
|
A:SER208
|
4.9
|
33.5
|
1.0
|
|
HA
|
A:ASP204
|
4.9
|
20.4
|
1.0
|
|
N
|
A:SER208
|
4.9
|
27.9
|
1.0
|
|
HB2
|
A:ASP187
|
4.9
|
22.1
|
1.0
|
|
OD1
|
A:ASP207
|
4.9
|
24.0
|
1.0
|
|
H
|
A:ASP207
|
4.9
|
27.9
|
1.0
|
|
HB2
|
A:HIS189
|
4.9
|
16.6
|
1.0
|
|
Calcium binding site 3 out
of 6 in 5w7e
Go back to
Calcium Binding Sites List in 5w7e
Calcium binding site 3 out
of 6 in the Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca603
b:19.2
occ:1.00
|
OD2
|
A:ASP226
|
2.2
|
24.7
|
1.0
|
|
OE2
|
A:GLU244
|
2.2
|
27.8
|
1.0
|
|
OD2
|
A:ASP222
|
2.3
|
27.5
|
1.0
|
|
O
|
A:ILE232
|
2.3
|
28.0
|
1.0
|
|
OD1
|
A:ASN228
|
2.3
|
22.7
|
1.0
|
|
OD1
|
A:ASN230
|
2.4
|
22.0
|
1.0
|
|
CG
|
A:ASN230
|
3.3
|
21.7
|
1.0
|
|
H
|
A:ILE232
|
3.3
|
29.0
|
1.0
|
|
CD
|
A:GLU244
|
3.4
|
28.1
|
1.0
|
|
HD21
|
A:ASN230
|
3.4
|
27.6
|
1.0
|
|
CG
|
A:ASP226
|
3.4
|
24.9
|
1.0
|
|
CG
|
A:ASN228
|
3.4
|
23.8
|
1.0
|
|
CG
|
A:ASP222
|
3.5
|
29.6
|
1.0
|
|
HA
|
A:ASP226
|
3.5
|
33.8
|
1.0
|
|
H
|
A:ASN228
|
3.5
|
31.4
|
1.0
|
|
C
|
A:ILE232
|
3.5
|
28.3
|
1.0
|
|
HB
|
A:ILE232
|
3.5
|
30.0
|
1.0
|
|
H
|
A:ASN230
|
3.6
|
23.3
|
1.0
|
|
HD21
|
A:ASN228
|
3.6
|
29.6
|
1.0
|
|
ND2
|
A:ASN230
|
3.7
|
23.0
|
1.0
|
|
HB3
|
A:ASP222
|
3.9
|
37.4
|
1.0
|
|
ND2
|
A:ASN228
|
3.9
|
24.6
|
1.0
|
|
HB3
|
A:TRP233
|
3.9
|
37.5
|
1.0
|
|
OE1
|
A:GLU244
|
3.9
|
29.3
|
1.0
|
|
N
|
A:ILE232
|
4.0
|
24.1
|
1.0
|
|
HB2
|
A:ASP222
|
4.0
|
37.4
|
1.0
|
|
HB2
|
A:GLU244
|
4.0
|
36.6
|
1.0
|
|
CB
|
A:ASP222
|
4.1
|
31.2
|
1.0
|
|
CA
|
A:ILE232
|
4.1
|
25.8
|
1.0
|
|
CA
|
A:ASP226
|
4.2
|
28.2
|
1.0
|
|
OD1
|
A:ASP226
|
4.2
|
23.6
|
1.0
|
|
CB
|
A:ILE232
|
4.3
|
25.0
|
1.0
|
|
CB
|
A:ASP226
|
4.3
|
27.0
|
1.0
|
|
HB3
|
A:PRO218
|
4.3
|
44.9
|
1.0
|
|
N
|
A:ASN228
|
4.3
|
26.1
|
1.0
|
|
H
|
A:SER227
|
4.3
|
33.0
|
1.0
|
|
N
|
A:ASN230
|
4.3
|
19.4
|
1.0
|
|
H
|
A:CYS229
|
4.4
|
25.8
|
1.0
|
|
H
|
A:GLY231
|
4.4
|
26.2
|
1.0
|
|
OD1
|
A:ASP222
|
4.5
|
29.8
|
1.0
|
|
HB2
|
A:ASP226
|
4.5
|
32.4
|
1.0
|
|
HD22
|
A:ASN230
|
4.5
|
27.6
|
1.0
|
|
C
|
A:ASP226
|
4.5
|
27.0
|
1.0
|
|
CB
|
A:ASN230
|
4.5
|
18.6
|
1.0
|
|
HA
|
A:TRP233
|
4.5
|
37.7
|
1.0
|
|
N
|
A:SER227
|
4.6
|
27.5
|
1.0
|
|
CG
|
A:GLU244
|
4.6
|
31.2
|
1.0
|
|
N
|
A:TRP233
|
4.6
|
29.5
|
1.0
|
|
HG22
|
A:ILE232
|
4.6
|
32.1
|
1.0
|
|
N
|
A:CYS229
|
4.7
|
21.5
|
1.0
|
|
N
|
A:GLY231
|
4.7
|
21.8
|
1.0
|
|
CB
|
A:ASN228
|
4.7
|
21.5
|
1.0
|
|
CB
|
A:GLU244
|
4.8
|
30.5
|
1.0
|
|
CB
|
A:TRP233
|
4.8
|
31.2
|
1.0
|
|
HD22
|
A:ASN228
|
4.8
|
29.6
|
1.0
|
|
CA
|
A:ASN230
|
4.8
|
18.6
|
1.0
|
|
CA
|
A:ASN228
|
4.8
|
22.8
|
1.0
|
|
C
|
A:ASN228
|
4.8
|
22.2
|
1.0
|
|
HG2
|
A:GLU244
|
4.8
|
37.5
|
1.0
|
|
C
|
A:ASN230
|
4.9
|
21.1
|
1.0
|
|
HB3
|
A:ASN230
|
4.9
|
22.3
|
1.0
|
|
CA
|
A:TRP233
|
4.9
|
31.4
|
1.0
|
|
HG
|
A:SER227
|
4.9
|
36.5
|
1.0
|
|
Calcium binding site 4 out
of 6 in 5w7e
Go back to
Calcium Binding Sites List in 5w7e
Calcium binding site 4 out
of 6 in the Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca601
b:19.0
occ:1.00
|
OD1
|
B:ASP187
|
2.2
|
20.1
|
1.0
|
|
OD1
|
B:ASP207
|
2.3
|
27.0
|
1.0
|
|
OD1
|
B:ASP183
|
2.3
|
22.2
|
1.0
|
|
OD1
|
B:ASP185
|
2.3
|
21.5
|
1.0
|
|
OD2
|
B:ASP204
|
2.3
|
22.0
|
1.0
|
|
O
|
B:HIS189
|
2.3
|
15.9
|
1.0
|
|
CG
|
B:ASP185
|
3.2
|
25.3
|
1.0
|
|
CG
|
B:ASP187
|
3.2
|
24.1
|
1.0
|
|
H
|
B:ASP187
|
3.3
|
27.8
|
1.0
|
|
HA
|
B:ASP207
|
3.4
|
34.9
|
1.0
|
|
CG
|
B:ASP207
|
3.4
|
28.0
|
1.0
|
|
HA
|
B:ASP183
|
3.4
|
25.5
|
1.0
|
|
OD2
|
B:ASP185
|
3.5
|
26.9
|
1.0
|
|
H
|
B:HIS189
|
3.5
|
21.7
|
1.0
|
|
CG
|
B:ASP204
|
3.5
|
20.5
|
1.0
|
|
C
|
B:HIS189
|
3.5
|
18.9
|
1.0
|
|
CG
|
B:ASP183
|
3.5
|
20.2
|
1.0
|
|
H
|
B:ASP185
|
3.6
|
30.7
|
1.0
|
|
HB2
|
B:ASP204
|
3.7
|
24.2
|
1.0
|
|
OD2
|
B:ASP187
|
3.7
|
26.1
|
1.0
|
|
CB
|
B:ASP204
|
4.0
|
20.2
|
1.0
|
|
HA
|
B:SER190
|
4.0
|
21.3
|
1.0
|
|
HB3
|
B:ASP204
|
4.0
|
24.2
|
1.0
|
|
HB2
|
B:HIS189
|
4.1
|
22.9
|
1.0
|
|
N
|
B:HIS189
|
4.1
|
18.1
|
1.0
|
|
N
|
B:ASP187
|
4.1
|
23.2
|
1.0
|
|
CA
|
B:ASP183
|
4.1
|
21.3
|
1.0
|
|
CA
|
B:CA602
|
4.2
|
24.1
|
1.0
|
|
CA
|
B:ASP207
|
4.2
|
29.1
|
1.0
|
|
CB
|
B:ASP207
|
4.2
|
30.8
|
1.0
|
|
H
|
B:SER186
|
4.2
|
30.9
|
1.0
|
|
HB2
|
B:ASP207
|
4.3
|
36.9
|
1.0
|
|
H
|
B:VAL184
|
4.3
|
32.3
|
1.0
|
|
CA
|
B:HIS189
|
4.3
|
17.7
|
1.0
|
|
H
|
B:LYS188
|
4.3
|
18.0
|
1.0
|
|
OD2
|
B:ASP207
|
4.3
|
26.9
|
1.0
|
|
N
|
B:ASP185
|
4.3
|
25.6
|
1.0
|
|
CB
|
B:ASP183
|
4.4
|
20.3
|
1.0
|
|
C
|
B:ASP183
|
4.4
|
24.1
|
1.0
|
|
OD2
|
B:ASP183
|
4.4
|
19.5
|
1.0
|
|
CB
|
B:ASP187
|
4.5
|
19.5
|
1.0
|
|
N
|
B:VAL184
|
4.5
|
26.9
|
1.0
|
|
N
|
B:SER190
|
4.5
|
15.4
|
1.0
|
|
N
|
B:SER186
|
4.5
|
25.8
|
1.0
|
|
OD1
|
B:ASP204
|
4.5
|
21.5
|
1.0
|
|
CB
|
B:ASP185
|
4.5
|
24.1
|
1.0
|
|
HB2
|
B:ASP183
|
4.6
|
24.4
|
1.0
|
|
N
|
B:LYS188
|
4.6
|
15.0
|
1.0
|
|
CA
|
B:ASP187
|
4.7
|
17.5
|
1.0
|
|
CB
|
B:HIS189
|
4.7
|
19.1
|
1.0
|
|
CA
|
B:SER190
|
4.7
|
17.8
|
1.0
|
|
O
|
B:ASP207
|
4.7
|
33.0
|
1.0
|
|
CA
|
B:ASP185
|
4.8
|
23.1
|
1.0
|
|
C
|
B:ASP185
|
4.8
|
25.1
|
1.0
|
|
C
|
B:ASP187
|
4.9
|
15.3
|
1.0
|
|
HB3
|
B:ASP187
|
4.9
|
23.4
|
1.0
|
|
C
|
B:ASP207
|
4.9
|
33.5
|
1.0
|
|
O
|
B:HOH884
|
5.0
|
28.5
|
1.0
|
|
HB3
|
B:ASP185
|
5.0
|
28.9
|
1.0
|
|
Calcium binding site 5 out
of 6 in 5w7e
Go back to
Calcium Binding Sites List in 5w7e
Calcium binding site 5 out
of 6 in the Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca602
b:24.1
occ:1.00
|
O
|
B:VAL212
|
2.3
|
22.7
|
1.0
|
|
O
|
B:ASN206
|
2.3
|
24.4
|
1.0
|
|
OD2
|
B:ASP187
|
2.3
|
26.1
|
1.0
|
|
OD2
|
B:ASP185
|
2.4
|
26.9
|
1.0
|
|
O
|
B:ASP209
|
2.5
|
25.4
|
1.0
|
|
OD2
|
B:ASP204
|
2.5
|
22.0
|
1.0
|
|
OD1
|
B:ASP204
|
2.5
|
21.5
|
1.0
|
|
CG
|
B:ASP204
|
2.9
|
20.5
|
1.0
|
|
HA
|
B:ASP207
|
3.2
|
34.9
|
1.0
|
|
CG
|
B:ASP187
|
3.4
|
24.1
|
1.0
|
|
C
|
B:ASN206
|
3.4
|
26.8
|
1.0
|
|
H
|
B:ASP209
|
3.4
|
42.2
|
1.0
|
|
CG
|
B:ASP185
|
3.4
|
25.3
|
1.0
|
|
C
|
B:VAL212
|
3.4
|
23.8
|
1.0
|
|
C
|
B:ASP209
|
3.5
|
29.6
|
1.0
|
|
HB
|
B:VAL212
|
3.5
|
34.3
|
1.0
|
|
H
|
B:VAL212
|
3.7
|
32.6
|
1.0
|
|
HA
|
B:LYS210
|
3.7
|
35.8
|
1.0
|
|
HB3
|
B:TYR213
|
3.8
|
25.2
|
1.0
|
|
H
|
B:ASN206
|
3.8
|
33.2
|
1.0
|
|
OD1
|
B:ASP187
|
3.8
|
20.1
|
1.0
|
|
OD1
|
B:ASP185
|
3.9
|
21.5
|
1.0
|
|
CA
|
B:ASP207
|
4.0
|
29.1
|
1.0
|
|
HB3
|
B:ASP209
|
4.1
|
39.5
|
1.0
|
|
N
|
B:ASP207
|
4.1
|
29.1
|
1.0
|
|
HB3
|
B:ASN206
|
4.1
|
43.6
|
1.0
|
|
CA
|
B:CA601
|
4.2
|
19.0
|
1.0
|
|
N
|
B:ASP209
|
4.2
|
35.2
|
1.0
|
|
CA
|
B:VAL212
|
4.2
|
26.4
|
1.0
|
|
CB
|
B:VAL212
|
4.3
|
28.6
|
1.0
|
|
N
|
B:VAL212
|
4.3
|
27.2
|
1.0
|
|
CA
|
B:ASP209
|
4.3
|
32.4
|
1.0
|
|
N
|
B:LYS210
|
4.4
|
28.1
|
1.0
|
|
CB
|
B:ASP204
|
4.4
|
20.2
|
1.0
|
|
HG12
|
B:VAL212
|
4.4
|
32.9
|
1.0
|
|
CA
|
B:LYS210
|
4.4
|
29.8
|
1.0
|
|
CA
|
B:ASN206
|
4.4
|
29.4
|
1.0
|
|
HA
|
B:TYR213
|
4.5
|
24.8
|
1.0
|
|
HB3
|
B:ASP187
|
4.5
|
23.4
|
1.0
|
|
N
|
B:ASN206
|
4.5
|
27.7
|
1.0
|
|
N
|
B:TYR213
|
4.5
|
23.8
|
1.0
|
|
HD2
|
B:TYR213
|
4.5
|
32.6
|
1.0
|
|
CB
|
B:ASP187
|
4.6
|
19.5
|
1.0
|
|
C
|
B:ASP207
|
4.6
|
33.5
|
1.0
|
|
CB
|
B:TYR213
|
4.7
|
21.0
|
1.0
|
|
CB
|
B:ASP185
|
4.7
|
24.1
|
1.0
|
|
CB
|
B:ASP209
|
4.7
|
32.9
|
1.0
|
|
HB2
|
B:ASP204
|
4.7
|
24.2
|
1.0
|
|
HB2
|
B:ASP185
|
4.7
|
28.9
|
1.0
|
|
CB
|
B:ASN206
|
4.8
|
36.4
|
1.0
|
|
CA
|
B:TYR213
|
4.8
|
20.7
|
1.0
|
|
H
|
B:SER208
|
4.8
|
44.3
|
1.0
|
|
HB3
|
B:ASP185
|
4.8
|
28.9
|
1.0
|
|
C
|
B:LYS210
|
4.8
|
29.0
|
1.0
|
|
HB3
|
B:ASP204
|
4.8
|
24.2
|
1.0
|
|
N
|
B:SER208
|
4.8
|
36.9
|
1.0
|
|
CG1
|
B:VAL212
|
4.9
|
27.4
|
1.0
|
|
HA
|
B:ASP204
|
4.9
|
27.8
|
1.0
|
|
HB2
|
B:ASP187
|
4.9
|
23.4
|
1.0
|
|
H
|
B:ASP207
|
4.9
|
35.0
|
1.0
|
|
OD1
|
B:ASP207
|
5.0
|
27.0
|
1.0
|
|
HB2
|
B:HIS189
|
5.0
|
22.9
|
1.0
|
|
Calcium binding site 6 out
of 6 in 5w7e
Go back to
Calcium Binding Sites List in 5w7e
Calcium binding site 6 out
of 6 in the Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Murine Acyloxyacyl Hydrolase (Aoah), S262A Mutant, with Dimyristoyl Phosphatidylcholine within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca603
b:23.5
occ:1.00
|
OD2
|
B:ASP226
|
2.2
|
30.4
|
1.0
|
|
OE2
|
B:GLU244
|
2.3
|
32.8
|
1.0
|
|
OD1
|
B:ASN228
|
2.3
|
29.4
|
1.0
|
|
O
|
B:ILE232
|
2.3
|
34.5
|
1.0
|
|
OD1
|
B:ASN230
|
2.4
|
28.1
|
1.0
|
|
OD2
|
B:ASP222
|
2.4
|
33.7
|
1.0
|
|
H
|
B:ILE232
|
3.3
|
37.2
|
1.0
|
|
CG
|
B:ASN230
|
3.3
|
27.5
|
1.0
|
|
CG
|
B:ASP226
|
3.4
|
30.3
|
1.0
|
|
HD21
|
B:ASN230
|
3.4
|
33.9
|
1.0
|
|
CG
|
B:ASN228
|
3.4
|
28.2
|
1.0
|
|
HB
|
B:ILE232
|
3.4
|
39.4
|
1.0
|
|
CD
|
B:GLU244
|
3.4
|
32.9
|
1.0
|
|
C
|
B:ILE232
|
3.5
|
35.2
|
1.0
|
|
CG
|
B:ASP222
|
3.5
|
36.0
|
1.0
|
|
HA
|
B:ASP226
|
3.5
|
42.0
|
1.0
|
|
H
|
B:ASN228
|
3.5
|
35.7
|
1.0
|
|
H
|
B:ASN230
|
3.6
|
28.9
|
1.0
|
|
HD21
|
B:ASN228
|
3.7
|
33.3
|
1.0
|
|
ND2
|
B:ASN230
|
3.7
|
28.2
|
1.0
|
|
HB2
|
B:ASP222
|
3.8
|
46.1
|
1.0
|
|
HB3
|
B:ASP222
|
3.8
|
46.1
|
1.0
|
|
N
|
B:ILE232
|
3.9
|
31.0
|
1.0
|
|
ND2
|
B:ASN228
|
4.0
|
27.7
|
1.0
|
|
CB
|
B:ASP222
|
4.0
|
38.5
|
1.0
|
|
OE1
|
B:GLU244
|
4.0
|
32.5
|
1.0
|
|
HB3
|
B:TRP233
|
4.0
|
46.8
|
1.0
|
|
CA
|
B:ILE232
|
4.0
|
33.4
|
1.0
|
|
HB2
|
B:GLU244
|
4.1
|
45.4
|
1.0
|
|
OD1
|
B:ASP226
|
4.2
|
28.7
|
1.0
|
|
CB
|
B:ILE232
|
4.2
|
32.8
|
1.0
|
|
CA
|
B:ASP226
|
4.2
|
35.0
|
1.0
|
|
H
|
B:SER227
|
4.2
|
39.0
|
1.0
|
|
N
|
B:ASN230
|
4.3
|
24.1
|
1.0
|
|
CB
|
B:ASP226
|
4.3
|
32.4
|
1.0
|
|
HB3
|
B:PRO218
|
4.3
|
46.6
|
1.0
|
|
N
|
B:ASN228
|
4.4
|
29.8
|
1.0
|
|
H
|
B:CYS229
|
4.4
|
33.4
|
1.0
|
|
H
|
B:GLY231
|
4.4
|
31.8
|
1.0
|
|
HD22
|
B:ASN230
|
4.5
|
33.9
|
1.0
|
|
HA
|
B:TRP233
|
4.5
|
47.7
|
1.0
|
|
N
|
B:SER227
|
4.5
|
32.5
|
1.0
|
|
C
|
B:ASP226
|
4.5
|
31.9
|
1.0
|
|
CB
|
B:ASN230
|
4.5
|
23.8
|
1.0
|
|
OD1
|
B:ASP222
|
4.6
|
36.3
|
1.0
|
|
HB2
|
B:ASP226
|
4.6
|
38.9
|
1.0
|
|
N
|
B:TRP233
|
4.6
|
37.7
|
1.0
|
|
HG22
|
B:ILE232
|
4.6
|
42.5
|
1.0
|
|
N
|
B:GLY231
|
4.6
|
26.5
|
1.0
|
|
N
|
B:CYS229
|
4.6
|
27.8
|
1.0
|
|
CG
|
B:GLU244
|
4.6
|
36.1
|
1.0
|
|
CB
|
B:ASN228
|
4.7
|
25.7
|
1.0
|
|
CA
|
B:ASN230
|
4.8
|
23.5
|
1.0
|
|
C
|
B:ASN228
|
4.8
|
27.8
|
1.0
|
|
HD22
|
B:ASN228
|
4.8
|
33.3
|
1.0
|
|
C
|
B:ASN230
|
4.8
|
26.0
|
1.0
|
|
CA
|
B:ASN228
|
4.8
|
28.0
|
1.0
|
|
CB
|
B:GLU244
|
4.8
|
37.8
|
1.0
|
|
CB
|
B:TRP233
|
4.8
|
39.0
|
1.0
|
|
CA
|
B:TRP233
|
4.9
|
39.7
|
1.0
|
|
HB3
|
B:ASN230
|
4.9
|
28.6
|
1.0
|
|
HG
|
B:SER227
|
4.9
|
43.3
|
1.0
|
|
C
|
B:GLY231
|
4.9
|
32.3
|
1.0
|
|
HG2
|
B:GLU244
|
5.0
|
43.4
|
1.0
|
|
HA
|
B:ILE232
|
5.0
|
40.0
|
1.0
|
|
CG2
|
B:ILE232
|
5.0
|
35.4
|
1.0
|
|
Reference:
A.Gorelik,
K.Illes,
B.Nagar.
Crystal Structure of the Mammalian Lipopolysaccharide Detoxifier. Proc. Natl. Acad. Sci. V. 115 E896 2018U.S.A..
ISSN: ESSN 1091-6490
PubMed: 29343645
DOI: 10.1073/PNAS.1719834115
Page generated: Mon Jul 15 13:03:44 2024
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