Calcium in PDB 5wsv: Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam

The structure of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam, PDB code: 5wsv was solved by J.Li, Y.Chen, Y.Deng, Q.Lu, M.Zhang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.88 / 2.33
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	38.683, 89.755, 45.724, 90.00, 106.03, 90.00
R / Rfree (%)	22.8 / 26.2

The binding sites of Calcium atom in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam (pdb code 5wsv). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam, PDB code: 5wsv:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Calcium binding site 1 out of 8 in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca201 b:48.1 occ:1.00 OD1 A:ASP20 2.1 39.2 1.0 OD1 A:ASP22 2.3 62.7 1.0 OE1 A:GLU31 2.3 42.6 1.0 O A:THR26 2.5 46.4 1.0 OD1 A:ASP24 2.6 60.2 1.0 OE2 A:GLU31 2.7 46.0 1.0 CD A:GLU31 2.8 43.2 1.0 OD2 A:ASP24 2.9 60.9 1.0 CG A:ASP24 3.0 58.5 1.0 CG A:ASP22 3.3 61.3 1.0 CG A:ASP20 3.3 40.4 1.0 OD2 A:ASP22 3.6 66.0 1.0 C A:THR26 3.6 45.5 1.0 CA A:ASP20 4.1 38.8 1.0 OD2 A:ASP20 4.1 40.4 1.0 OG1 A:THR26 4.1 46.4 1.0 N A:ASP24 4.2 56.4 1.0 CB A:ASP20 4.2 40.1 1.0 CB A:ASP24 4.2 56.3 1.0 N A:ASP22 4.3 52.2 1.0 N A:THR26 4.4 47.8 1.0 CG A:GLU31 4.4 42.1 1.0 C A:ASP20 4.4 38.1 1.0 N A:ILE27 4.5 42.7 1.0 N A:GLY23 4.5 56.8 1.0 CA A:THR26 4.5 46.7 1.0 CA A:ILE27 4.5 42.0 1.0 N A:LYS21 4.5 39.7 1.0 CB A:ASP22 4.6 60.2 1.0 CG2 A:THR28 4.7 49.8 1.0 CA A:ASP24 4.7 55.3 1.0 CA A:ASP22 4.8 56.1 1.0 N A:THR28 4.8 45.2 1.0 C A:ASP22 4.9 56.2 1.0 N A:GLY25 4.9 52.0 1.0 O A:ASP20 5.0 36.6 1.0 CB A:THR26 5.0 47.0 1.0

Calcium binding site 2 out of 8 in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca202 b:49.4 occ:1.00 OD1 A:ASN60 2.3 64.7 1.0 OE2 A:GLU67 2.3 45.7 1.0 OD1 A:ASP56 2.4 53.2 1.0 OE1 A:GLU67 2.5 45.0 1.0 O A:THR62 2.6 52.3 1.0 ND2 A:ASN60 2.6 65.7 1.0 CD A:GLU67 2.7 43.9 1.0 OD1 A:ASP58 2.7 70.5 1.0 CG A:ASN60 2.7 65.0 1.0 OD2 A:ASP64 3.3 56.7 1.0 CG A:ASP56 3.5 52.2 1.0 CG A:ASP58 3.6 69.3 1.0 C A:THR62 3.7 52.0 1.0 OD2 A:ASP58 3.8 73.8 1.0 CG A:GLU67 4.2 44.3 1.0 CB A:ASN60 4.2 64.3 1.0 OD2 A:ASP56 4.2 54.9 1.0 N A:ASP64 4.3 48.1 1.0 CG A:ASP64 4.3 55.2 1.0 CB A:ASP56 4.3 50.9 1.0 CA A:ASP56 4.4 49.9 1.0 OG1 A:THR62 4.4 57.1 1.0 N A:THR62 4.4 55.6 1.0 CA A:ILE63 4.5 48.0 1.0 N A:ILE63 4.5 48.1 1.0 N A:ASN60 4.5 65.2 1.0 CA A:THR62 4.6 54.9 1.0 N A:ALA57 4.7 51.9 1.0 N A:ASP58 4.8 63.1 1.0 CA A:ASN60 4.8 64.1 1.0 C A:ASP56 4.8 50.5 1.0 C A:ILE63 4.8 46.9 1.0 CB A:ASP58 5.0 68.3 1.0 N A:GLY61 5.0 60.2 1.0

Calcium binding site 3 out of 8 in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca203 b:38.1 occ:1.00 O A:TYR99 2.3 41.0 1.0 OD1 A:ASP93 2.3 36.7 1.0 O A:HOH301 2.4 32.8 1.0 OE2 A:GLU104 2.4 36.8 1.0 OD1 A:ASN97 2.4 41.7 1.0 OD2 A:ASP95 2.4 34.0 1.0 OE1 A:GLU104 2.7 39.0 1.0 CD A:GLU104 2.9 36.3 1.0 CG A:ASN97 3.1 42.0 1.0 CG A:ASP95 3.1 34.4 1.0 OD1 A:ASP95 3.2 35.1 1.0 ND2 A:ASN97 3.4 43.5 1.0 C A:TYR99 3.4 41.4 1.0 CG A:ASP93 3.6 35.8 1.0 N A:TYR99 4.1 41.0 1.0 N A:ASN97 4.2 38.2 1.0 CA A:ASP93 4.2 33.6 1.0 CA A:TYR99 4.3 42.1 1.0 N A:ILE100 4.3 41.5 1.0 CB A:ASN97 4.3 41.5 1.0 N A:ASP95 4.3 33.0 1.0 CG A:GLU104 4.4 33.9 1.0 CB A:ASP93 4.4 34.3 1.0 CA A:ILE100 4.4 40.2 1.0 CB A:ASP95 4.5 34.3 1.0 OD2 A:ASP93 4.5 36.4 1.0 C A:ASP93 4.5 34.3 1.0 OE1 A:GLN135 4.5 64.8 1.0 N A:GLY96 4.5 35.4 1.0 N A:SER101 4.5 39.3 1.0 N A:LYS94 4.6 34.4 1.0 CA A:ASN97 4.7 40.1 1.0 N A:GLY98 4.8 40.1 1.0 CA A:ASP95 4.8 33.7 1.0 C A:ILE100 4.9 40.4 1.0 CB A:TYR99 4.9 43.6 1.0 C A:ASP95 4.9 34.3 1.0

Calcium binding site 4 out of 8 in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca204 b:53.6 occ:1.00 OD1 A:ASP133 2.2 57.6 1.0 OD1 A:ASP129 2.2 55.2 1.0 O A:GLN135 2.4 56.3 1.0 OD1 A:ASP131 2.4 73.8 1.0 OE2 A:GLU140 2.5 50.4 1.0 OE1 A:GLU140 2.6 50.9 1.0 CG A:ASP133 2.9 60.9 1.0 CD A:GLU140 2.9 49.0 1.0 OD2 A:ASP133 3.1 59.1 1.0 CG A:ASP131 3.2 70.2 1.0 CG A:ASP129 3.3 57.5 1.0 OD2 A:ASP131 3.4 71.0 1.0 C A:GLN135 3.5 55.1 1.0 OD2 A:ASP129 4.0 57.8 1.0 ND2 A:ASN137 4.0 48.9 1.0 CB A:ASP133 4.2 62.4 1.0 CA A:VAL136 4.2 46.2 1.0 N A:VAL136 4.2 49.1 1.0 N A:GLN135 4.2 60.5 1.0 N A:ASP133 4.3 63.9 1.0 N A:ASN137 4.4 44.3 1.0 CG A:GLU140 4.4 47.5 1.0 CA A:GLN135 4.4 59.0 1.0 CB A:ASP129 4.5 58.5 1.0 N A:ASP131 4.5 67.2 1.0 CA A:ASP129 4.5 59.1 1.0 CB A:ASP131 4.6 69.5 1.0 CA A:ASP133 4.7 62.8 1.0 CG A:ASN137 4.7 47.6 1.0 N A:ILE130 4.7 62.4 1.0 N A:GLY132 4.8 65.7 1.0 C A:VAL136 4.8 44.5 1.0 N A:GLY134 4.8 62.3 1.0 CA A:ASP131 4.9 68.1 1.0 C A:ASP129 5.0 60.9 1.0 C A:ASP133 5.0 62.4 1.0 CB A:GLN135 5.0 61.1 1.0

Calcium binding site 5 out of 8 in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca201 b:48.5 occ:1.00 OD1 C:ASP22 2.2 49.3 1.0 OD1 C:ASP24 2.3 46.0 1.0 O C:THR26 2.4 49.3 1.0 OE2 C:GLU31 2.4 40.8 1.0 OD1 C:ASP20 2.6 38.9 1.0 OE1 C:GLU31 3.1 42.8 1.0 CD C:GLU31 3.1 42.2 1.0 CG C:ASP22 3.2 49.9 1.0 CG C:ASP24 3.2 48.1 1.0 OD2 C:ASP22 3.5 51.5 1.0 C C:THR26 3.5 48.0 1.0 OD2 C:ASP24 3.6 48.9 1.0 CG C:ASP20 3.7 41.1 1.0 OG1 C:THR26 4.0 58.6 1.0 CA C:ASP20 4.1 40.8 1.0 N C:THR26 4.2 47.9 1.0 N C:ASP24 4.3 48.4 1.0 CA C:THR26 4.4 50.3 1.0 C C:ASP20 4.4 42.1 1.0 N C:ASP22 4.4 49.8 1.0 N C:ILE27 4.4 45.9 1.0 N C:LYS21 4.4 44.1 1.0 CB C:ASP24 4.5 48.4 1.0 CA C:ILE27 4.5 45.0 1.0 CB C:ASP20 4.5 40.1 1.0 CG C:GLU31 4.5 42.9 1.0 CB C:ASP22 4.5 50.3 1.0 N C:GLY23 4.6 47.2 1.0 OD2 C:ASP20 4.7 44.1 1.0 CA C:ASP24 4.8 48.4 1.0 N C:GLY25 4.8 47.2 1.0 CB C:THR26 4.8 53.9 1.0 CA C:ASP22 4.9 49.6 1.0 C C:ASP22 4.9 49.1 1.0 C C:ASP24 5.0 47.9 1.0 O C:ASP20 5.0 42.2 1.0

Calcium binding site 6 out of 8 in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca202 b:60.0 occ:1.00 OD1 C:ASN60 2.1 64.4 1.0 O C:HOH304 2.4 45.5 1.0 OE2 C:GLU67 2.4 45.6 1.0 O C:THR62 2.5 56.7 1.0 OE1 C:GLU67 2.5 47.5 1.0 CD C:GLU67 2.8 45.8 1.0 OD1 C:ASP56 2.9 68.2 1.0 OD1 C:ASP58 2.9 71.0 1.0 CG C:ASN60 2.9 65.1 1.0 ND2 C:ASN60 3.2 66.0 1.0 CG C:ASP56 3.4 65.0 1.0 C C:THR62 3.5 54.8 1.0 OD2 C:ASP56 3.7 63.6 1.0 CG C:ASP58 3.8 73.5 1.0 OD2 C:ASP58 3.9 75.5 1.0 CB C:ASN60 4.2 66.6 1.0 CA C:ILE63 4.2 51.8 1.0 N C:ILE63 4.2 51.6 1.0 CG C:GLU67 4.3 44.5 1.0 N C:THR62 4.3 56.0 1.0 CB C:ASP56 4.3 63.3 1.0 OG1 C:THR62 4.4 52.8 1.0 N C:ASP64 4.4 49.0 1.0 N C:ASN60 4.5 68.1 1.0 CA C:THR62 4.5 55.1 1.0 OD1 C:ASP64 4.6 59.6 1.0 CA C:ASP56 4.6 62.4 1.0 C C:ILE63 4.6 49.8 1.0 CG C:ASP64 4.7 55.8 1.0 N C:ALA57 4.7 65.1 1.0 CA C:ASN60 4.8 66.7 1.0 N C:ASP58 4.8 72.5 1.0 N C:GLY61 5.0 59.6 1.0

Calcium binding site 7 out of 8 in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca203 b:36.0 occ:1.00 O C:TYR99 2.2 31.0 1.0 OD1 C:ASP93 2.2 33.1 1.0 OD1 C:ASN97 2.3 33.0 1.0 OD1 C:ASP95 2.4 39.1 1.0 OE2 C:GLU104 2.5 39.5 1.0 OE1 C:GLU104 2.5 41.2 1.0 CD C:GLU104 2.9 40.2 1.0 CG C:ASP95 3.2 39.1 1.0 CG C:ASN97 3.2 33.7 1.0 OD2 C:ASP95 3.3 40.0 1.0 C C:TYR99 3.4 30.7 1.0 CG C:ASP93 3.4 30.8 1.0 ND2 C:ASN97 3.6 34.1 1.0 N C:TYR99 4.0 32.2 1.0 N C:ASN97 4.1 33.7 1.0 OD2 C:ASP93 4.2 29.5 1.0 CA C:TYR99 4.2 31.7 1.0 CG C:GLU104 4.4 38.1 1.0 CB C:ASP93 4.4 31.6 1.0 N C:ILE100 4.4 30.3 1.0 N C:GLY96 4.4 34.8 1.0 CA C:ASP93 4.4 32.0 1.0 CB C:ASN97 4.4 34.4 1.0 CA C:ILE100 4.5 30.5 1.0 N C:ASP95 4.5 37.4 1.0 CB C:ASP95 4.6 39.2 1.0 CB C:TYR99 4.7 32.8 1.0 N C:SER101 4.7 31.6 1.0 CA C:ASN97 4.7 33.8 1.0 C C:ASP93 4.7 32.3 1.0 N C:GLY98 4.7 33.5 1.0 N C:LYS94 4.8 33.3 1.0 CA C:ASP95 4.9 37.4 1.0 C C:ASN97 4.9 33.3 1.0

Calcium binding site 8 out of 8 in the Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Crystal Structure of Myosin Viia IQ5 in Complex with CA2+-Cam within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca204 b:42.4 occ:1.00 OD1 C:ASP129 1.8 49.9 1.0 OD1 C:ASP133 2.2 45.4 1.0 OD1 C:ASP131 2.3 48.6 1.0 O C:GLN135 2.4 37.8 1.0 OE1 C:GLU140 2.4 36.9 1.0 O C:HOH306 2.4 35.9 1.0 OE2 C:GLU140 2.7 36.4 1.0 CD C:GLU140 2.9 36.6 1.0 CG C:ASP129 3.0 48.9 1.0 CG C:ASP133 3.1 45.6 1.0 CG C:ASP131 3.2 49.9 1.0 OD2 C:ASP133 3.5 43.0 1.0 OD2 C:ASP131 3.5 49.0 1.0 C C:GLN135 3.6 38.0 1.0 OD2 C:ASP129 3.7 49.6 1.0 CB C:ASP129 4.1 47.4 1.0 CA C:ASP129 4.3 47.5 1.0 N C:ASP133 4.3 48.7 1.0 N C:GLN135 4.3 41.6 1.0 CA C:VAL136 4.3 33.1 1.0 N C:VAL136 4.4 35.0 1.0 N C:ASP131 4.4 48.6 1.0 CB C:ASP133 4.4 46.2 1.0 N C:ASN137 4.4 31.3 1.0 CG C:GLU140 4.4 36.2 1.0 CB C:ASP131 4.5 50.7 1.0 CA C:GLN135 4.5 41.3 1.0 N C:GLY132 4.7 50.6 1.0 N C:ILE130 4.7 49.9 1.0 C C:ASP129 4.8 48.8 1.0 CA C:ASP133 4.8 47.0 1.0 CG C:ASN137 4.8 32.4 1.0 CA C:ASP131 4.8 49.6 1.0 ND2 C:ASN137 4.9 32.8 1.0 C C:VAL136 4.9 30.9 1.0 C C:ASP131 4.9 49.7 1.0 N C:GLY134 4.9 47.0 1.0 OD1 C:ASN137 4.9 34.4 1.0

J.Li, Y.Chen, Y.Deng, I.C.Unarta, Q.Lu, X.Huang, M.Zhang. Ca(2+)-Induced Rigidity Change of the Myosin Viia Iq Motif-Single Alpha Helix Lever Arm Extension Structure V. 25 579 2017.
ISSN: ISSN 1878-4186
PubMed: 28262393
DOI: 10.1016/J.STR.2017.02.002