Calcium in PDB 5yl7: Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717)

The structure of Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717), PDB code: 5yl7 was solved by J.H.Lee, C.W.Lee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	47.948, 74.562, 83.012, 90.00, 90.00, 90.00
R / Rfree (%)	13 / 15.5

The binding sites of Calcium atom in the Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717) (pdb code 5yl7). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717), PDB code: 5yl7:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca801 b:6.5 occ:1.00 O A:VAL255 2.3 6.7 1.0 O A:GLU260 2.3 7.8 1.0 OD2 A:ASP149 2.4 8.3 1.0 O A:VAL262 2.4 6.4 1.0 OD1 A:ASP193 2.4 7.4 1.0 OD1 A:ASN258 2.5 8.7 1.0 OD2 A:ASP193 2.5 6.7 1.0 CG A:ASP193 2.8 7.4 1.0 CG A:ASP149 3.4 7.8 1.0 CG A:ASN258 3.4 7.1 1.0 C A:VAL255 3.5 5.8 1.0 C A:GLU260 3.5 8.0 1.0 C A:VAL262 3.6 5.7 1.0 N A:VAL262 3.7 6.7 1.0 ND2 A:ASN258 3.8 8.1 1.0 C A:GLY261 4.0 7.2 1.0 CB A:ASP149 4.1 9.2 1.0 CA A:VAL262 4.2 6.0 1.0 CA A:THR256 4.2 7.2 1.0 N A:THR256 4.2 6.0 1.0 CB A:ASP193 4.3 6.8 1.0 CA A:GLY261 4.4 7.4 1.0 OD1 A:ASP149 4.4 8.9 1.0 N A:GLY261 4.4 7.7 1.0 N A:GLU260 4.4 10.2 1.0 CA A:GLU260 4.4 10.5 1.0 CA A:VAL255 4.5 7.1 1.0 CB A:GLU260 4.6 12.9 1.0 O A:GLY261 4.6 7.3 1.0 N A:ALA263 4.7 5.9 1.0 N A:VAL255 4.8 6.3 1.0 CB A:VAL255 4.8 7.5 1.0 CB A:ASN258 4.8 8.7 1.0 N A:ASN258 4.8 8.7 1.0 C A:THR256 4.9 8.2 1.0 O A:ASP193 4.9 6.9 1.0 N A:ASN257 4.9 8.7 1.0 CA A:ALA263 5.0 5.6 1.0

Calcium binding site 2 out of 4 in the Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca802 b:7.6 occ:1.00 O A:GLY216 2.3 8.1 1.0 O A:HOH1308 2.3 11.0 1.0 O A:HOH1048 2.4 7.8 1.0 OD2 A:ASP218 2.4 6.7 1.0 O A:GLY215 2.4 8.7 1.0 OD1 A:ASP213 2.5 7.9 1.0 OD2 A:ASP213 2.5 7.4 1.0 CG A:ASP213 2.8 8.3 1.0 C A:GLY215 3.2 9.8 1.0 C A:GLY216 3.4 8.9 1.0 CG A:ASP218 3.5 7.1 1.0 O A:HOH902 3.5 0.7 1.0 N A:GLY216 3.8 10.2 1.0 CA A:CA803 3.9 5.9 1.0 CA A:GLY216 4.0 9.1 1.0 CA A:GLY215 4.1 11.5 1.0 CB A:ALA211 4.1 9.0 1.0 OD2 A:ASP203 4.1 6.1 1.0 N A:GLY215 4.1 10.6 1.0 CB A:ASP218 4.2 6.9 1.0 CB A:ASP213 4.3 7.6 1.0 O A:HOH1152 4.3 21.4 1.0 O A:HOH1363 4.3 89.7 1.0 N A:ARG217 4.4 7.9 1.0 OD1 A:ASP218 4.5 8.3 1.0 CG A:ASP203 4.5 5.3 1.0 O A:ILE205 4.5 8.3 1.0 C A:ARG217 4.6 8.1 1.0 O A:HOH1381 4.6 23.9 1.0 N A:ASP218 4.6 7.2 1.0 OD1 A:ASP203 4.6 6.8 1.0 O A:HOH1279 4.7 9.2 1.0 CA A:ARG217 4.7 7.8 1.0 C A:ASP213 4.9 8.9 1.0

Calcium binding site 3 out of 4 in the Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca803 b:5.9 occ:1.00 OD2 A:ASP213 2.3 7.4 1.0 O A:HOH1008 2.4 7.4 1.0 O A:HOH1279 2.4 9.2 1.0 OD2 A:ASP203 2.4 6.1 1.0 O A:ASP220 2.4 5.9 1.0 OD1 A:ASP218 2.5 8.3 1.0 OD2 A:ASP218 2.5 6.7 1.0 CG A:ASP218 2.9 7.1 1.0 CG A:ASP203 3.3 5.3 1.0 CG A:ASP213 3.5 8.3 1.0 C A:ASP220 3.6 5.9 1.0 CB A:ASP203 3.6 6.0 1.0 CA A:CA802 3.9 7.6 1.0 O A:HOH902 4.1 0.7 1.0 CB A:ASP220 4.1 6.9 1.0 CB A:ASP213 4.1 7.6 1.0 CA A:ASP220 4.3 6.6 1.0 NH1 A:ARG275 4.3 7.2 1.0 OD1 A:ASP203 4.4 6.8 1.0 N A:ASP220 4.4 6.3 1.0 CB A:ASP218 4.4 6.9 1.0 O A:HOH1152 4.4 21.4 1.0 O A:HOH1048 4.5 7.8 1.0 OD1 A:ASP213 4.5 7.9 1.0 O A:HOH1432 4.6 13.8 1.0 N A:ALA221 4.6 5.6 1.0 O A:ARG222 4.7 6.5 1.0 O A:HOH1305 4.7 15.4 1.0 O A:HOH1103 4.7 19.9 1.0 CG A:ASP220 4.7 9.4 1.0 O A:HOH1308 4.8 11.0 1.0 CA A:ALA221 4.9 5.9 1.0

Calcium binding site 4 out of 4 in the Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Proteases From Pseudoalteromonas Arctica Pamc 21717 (PRO21717) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca804 b:6.1 occ:1.00 O A:VAL362 2.3 5.6 1.0 O A:HOH1322 2.4 10.5 1.0 OD2 A:ASP385 2.4 6.7 1.0 O A:HOH1001 2.5 11.5 1.0 OE2 A:GLU451 2.5 8.0 1.0 O A:HOH1037 2.5 10.2 1.0 O A:HOH1281 2.5 9.2 1.0 OD1 A:ASP385 2.8 7.2 1.0 O A:HOH908 2.9 0.8 1.0 CG A:ASP385 3.0 6.1 1.0 O A:HOH1441 3.1 0.7 1.0 C A:VAL362 3.5 5.1 1.0 CD A:GLU451 3.6 6.8 1.0 O A:HOH1273 3.6 68.2 1.0 OE1 A:GLU451 3.9 8.3 1.0 N A:VAL362 4.2 5.5 1.0 O A:HOH1166 4.2 23.4 1.0 CB A:ASP385 4.4 5.3 1.0 O A:HOH1280 4.4 14.9 1.0 N A:VAL363 4.4 4.8 1.0 CA A:VAL363 4.4 5.0 1.0 CA A:VAL362 4.5 5.1 1.0 O A:HOH1463 4.5 24.1 1.0 O A:HOH981 4.6 10.6 1.0 O A:HOH1230 4.6 18.6 1.0 N A:ASN364 4.7 4.5 1.0 O A:HOH1267 4.7 13.1 1.0 O A:ASN383 4.8 7.7 1.0 CG A:GLU451 4.8 6.5 1.0 CG2 A:VAL362 4.9 6.8 1.0 NZ A:LYS455 4.9 10.4 1.0 O A:HOH1448 5.0 34.4 1.0

H.J.Park, C.W.Lee, D.Kim, H.Do, S.J.Han, J.E.Kim, B.H.Koo, J.H.Lee, J.H.Yim. Crystal Structure of A Cold-Active Protease (PRO21717) From the Psychrophilic Bacterium, Pseudoalteromonas Arctica Pamc 21717, at 1.4 Angstrom Resolution: Structural Adaptations to Cold and Functional Analysis of A Laundry Detergent Enzyme Plos One V. 13 91740 2018.
ISSN: ESSN 1932-6203
PubMed: 29466378
DOI: 10.1371/JOURNAL.PONE.0191740