Calcium in PDB 5zrs: Crystal Structure of Pet-Degrading Cutinase CUT190 S176A/S226P/R228S Mutant in Monoethyl Adipate Bound State

Protein crystallography data

The structure of Crystal Structure of Pet-Degrading Cutinase CUT190 S176A/S226P/R228S Mutant in Monoethyl Adipate Bound State, PDB code: 5zrs was solved by N.Numoto, N.Kamiya, G.J.Bekker, Y.Yamagami, S.Inaba, K.Ishii, S.Uchiyama, F.Kawai, N.Ito, M.Oda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.61 / 1.40
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 50.457, 63.856, 78.785, 90.00, 90.00, 90.00
R / Rfree (%) 16.1 / 19.2

Other elements in 5zrs:

The structure of Crystal Structure of Pet-Degrading Cutinase CUT190 S176A/S226P/R228S Mutant in Monoethyl Adipate Bound State also contains other interesting chemical elements:

Zinc (Zn) 3 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Pet-Degrading Cutinase CUT190 S176A/S226P/R228S Mutant in Monoethyl Adipate Bound State (pdb code 5zrs). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Pet-Degrading Cutinase CUT190 S176A/S226P/R228S Mutant in Monoethyl Adipate Bound State, PDB code: 5zrs:

Calcium binding site 1 out of 1 in 5zrs

Go back to Calcium Binding Sites List in 5zrs
Calcium binding site 1 out of 1 in the Crystal Structure of Pet-Degrading Cutinase CUT190 S176A/S226P/R228S Mutant in Monoethyl Adipate Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Pet-Degrading Cutinase CUT190 S176A/S226P/R228S Mutant in Monoethyl Adipate Bound State within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca405

b:25.5
occ:1.00
O A:SER76 2.3 22.6 1.0
O A:ALA78 2.3 18.5 1.0
O A:PHE81 2.3 13.2 1.0
O A:HOH772 2.4 25.3 1.0
O A:HOH660 2.4 21.2 1.0
O A:HOH617 2.6 19.5 1.0
C A:SER76 3.4 19.2 1.0
C A:PHE81 3.5 12.6 1.0
C A:ALA78 3.5 16.4 1.0
C A:PHE77 3.9 21.7 1.0
CA A:PHE77 3.9 19.6 1.0
N A:ALA78 4.0 17.3 1.0
OD1 A:ASN133 4.0 16.0 1.0
N A:PHE77 4.1 17.5 1.0
CA A:GLY82 4.1 12.0 1.0
N A:PHE81 4.2 13.3 1.0
N A:GLY82 4.3 11.7 1.0
O A:PHE77 4.3 20.1 1.0
CA A:ALA78 4.3 19.4 1.0
N A:SER79 4.5 16.4 1.0
CA A:PHE81 4.5 11.7 1.0
CA A:SER76 4.5 17.9 1.0
N A:GLY80 4.6 18.3 1.0
CA A:SER79 4.6 15.4 1.0
C A:SER79 4.7 15.8 1.0
C A:GLY82 4.8 15.1 1.0
N A:GLY83 4.9 13.7 1.0
O A:HOH724 4.9 36.6 1.0

Reference:

N.Numoto, N.Kamiya, G.J.Bekker, Y.Yamagami, S.Inaba, K.Ishii, S.Uchiyama, F.Kawai, N.Ito, M.Oda. Structural Dynamics of the Pet-Degrading Cutinase-Like Enzyme From Saccharomonospora Viridis AHK190 in Substrate-Bound States Elucidates the CA2+-Driven Catalytic Cycle. Biochemistry V. 57 5289 2018.
ISSN: ISSN 1520-4995
PubMed: 30110540
DOI: 10.1021/ACS.BIOCHEM.8B00624
Page generated: Sat Dec 12 05:56:03 2020

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