Calcium in PDB 6dbv: Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas

All present enzymatic activity of Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas:
2.3.2.27;

The structure of Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Calcium atom in the Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas (pdb code 6dbv). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas, PDB code: 6dbv:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1102 b:0.6 occ:1.00 CA A:CA1103 2.3 0.2 1.0 OE2 A:GLU984 2.5 0.6 1.0 OP1 E:DC17 2.5 94.3 1.0 OD2 A:ASP620 2.6 0.8 1.0 CD A:GLU984 2.7 1.0 1.0 OP2 E:DC17 3.0 0.0 1.0 CG A:GLU984 3.0 95.6 1.0 P E:DC17 3.1 82.9 1.0 NE2 A:HIS817 3.3 54.2 1.0 OE1 A:GLU984 3.5 0.8 1.0 CG A:ASP620 3.8 70.9 1.0 CE1 A:HIS817 3.8 46.0 1.0 CB A:GLU984 4.2 76.4 1.0 O5' E:DC17 4.2 82.9 1.0 OD1 A:ASP620 4.3 62.5 1.0 O3' E:DA16 4.4 69.3 1.0 CD2 A:HIS817 4.6 47.4 1.0 CG A:ASP730 4.8 69.1 1.0 CB A:ASP730 4.8 49.9 1.0 CB A:ASP620 4.9 48.8 1.0 OD2 A:ASP730 4.9 0.9 1.0

Calcium binding site 2 out of 4 in the Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1103 b:0.2 occ:1.00 CA A:CA1102 2.3 0.6 1.0 OE2 A:GLU984 2.4 0.6 1.0 OP2 E:DC17 2.5 0.0 1.0 CD A:GLU984 2.7 1.0 1.0 OE1 A:GLU984 2.7 0.8 1.0 P E:DC17 2.9 82.9 1.0 O5' E:DC17 3.1 82.9 1.0 OP1 E:DC17 3.1 94.3 1.0 CG A:GLU984 3.9 95.6 1.0 OD2 A:ASP620 4.2 0.8 1.0 C3' E:DC17 4.2 75.9 1.0 C5' E:DC17 4.4 74.3 1.0 O3' E:DA16 4.5 69.3 1.0 OP2 E:DA18 4.5 78.5 1.0 C2' E:DC17 4.5 76.1 1.0 NE2 A:HIS817 4.7 54.2 1.0 CB A:GLU984 4.8 76.4 1.0 C4' E:DC17 4.9 69.3 1.0

Calcium binding site 3 out of 4 in the Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca1102 b:0.6 occ:1.00 CA C:CA1103 2.1 0.5 1.0 OD2 C:ASP620 2.4 0.5 1.0 OE2 C:GLU984 3.1 0.9 1.0 OE1 C:GLU984 3.2 0.1 1.0 CG C:ASP620 3.2 79.8 1.0 OD1 C:ASP620 3.4 63.7 1.0 CD C:GLU984 3.5 1.0 1.0 O C:GLY621 3.6 43.2 1.0 OP1 H:DA47 4.2 0.6 1.0 CB C:ASP620 4.6 51.0 1.0 O5' H:DT46 4.7 97.5 1.0 C C:GLY621 4.8 31.6 1.0 OD2 C:ASP730 4.9 1.0 1.0

Calcium binding site 4 out of 4 in the Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Cryo-Em Structure of Rag in Complex with 12-Rss and 23-Rss Substrate Dnas within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca1103 b:0.5 occ:1.00 CA C:CA1102 2.1 0.6 1.0 OD2 C:ASP620 2.5 0.5 1.0 OD1 C:ASP620 2.8 63.7 1.0 CG C:ASP620 3.0 79.8 1.0 OD2 C:ASP730 3.2 1.0 1.0 OP1 H:DA47 4.0 0.6 1.0 O C:GLY621 4.2 43.2 1.0 CG C:ASP730 4.5 68.4 1.0 CB C:ASP620 4.5 51.0 1.0 OE1 C:GLU984 4.6 0.1 1.0 OE2 C:GLU684 4.8 0.1 1.0 OE2 C:GLU984 4.9 0.9 1.0 N C:GLY621 5.0 35.4 1.0

H.Ru, W.Mi, P.Zhang, F.W.Alt, D.G.Schatz, M.Liao, H.Wu. Dna Melting Initiates the Rag Catalytic Pathway. Nat. Struct. Mol. Biol. V. 25 732 2018.
ISSN: ESSN 1545-9985
PubMed: 30061602
DOI: 10.1038/S41594-018-0098-5