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Calcium in PDB 6dmn: Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C

Enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C

All present enzymatic activity of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C:
3.1.26.4;

Protein crystallography data

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C, PDB code: 6dmn was solved by N.L.Samara, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.86 / 1.27
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 81.465, 37.681, 62.022, 90.00, 95.93, 90.00
R / Rfree (%) 15 / 17.1

Other elements in 6dmn:

The structure of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C also contains other interesting chemical elements:

Potassium (K) 1 atom
Iodine (I) 4 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C (pdb code 6dmn). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C, PDB code: 6dmn:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 6dmn

Go back to Calcium Binding Sites List in 6dmn
Calcium binding site 1 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca201

b:17.7
occ:0.75
OD1 A:ASP192 2.2 24.7 1.0
O A:HOH311 2.2 25.8 0.8
O A:HOH340 2.3 23.5 0.8
OD2 A:ASP71 2.3 22.1 1.0
OP1 b:C5 2.4 18.6 1.0
O A:HOH302 2.4 21.5 0.8
O1 A:EDO211 2.5 24.8 0.8
CG A:ASP192 3.3 25.1 1.0
HA A:ASP192 3.3 29.0 1.0
CG A:ASP71 3.4 18.1 1.0
P b:C5 3.5 18.1 1.0
C1 A:EDO211 3.7 27.3 0.8
H A:TYR193 3.7 33.0 1.0
O A:HOH302 3.7 18.6 0.2
OD1 A:ASP71 3.7 19.2 1.0
OP2 b:G6 3.8 15.6 0.2
OP2 b:C5 3.9 21.2 1.0
HB2 A:ASP192 4.0 28.6 1.0
CB A:ASP192 4.0 23.8 1.0
C2 A:EDO211 4.1 29.1 0.8
CA A:ASP192 4.1 24.2 1.0
OD2 A:ASP192 4.2 28.6 1.0
CA A:CA202 4.2 16.5 0.9
H A:GLY194 4.3 37.7 1.0
HG22 A:ILE189 4.3 25.8 1.0
O5' b:C5 4.3 17.4 1.0
O A:VAL72 4.3 17.6 1.0
OE2 A:GLU188 4.3 31.4 1.0
N A:TYR193 4.4 27.5 1.0
O A:HOH307 4.4 43.9 1.0
O2 A:EDO211 4.6 29.5 0.8
OD2 A:ASP132 4.6 19.0 1.0
C5' b:C5 4.7 17.4 1.0
CB A:ASP71 4.7 17.7 1.0
HB3 A:ASP71 4.7 21.2 1.0
O3' B:U4 4.7 17.9 1.0
C A:ASP192 4.8 22.8 1.0
O b:HOH103 4.8 29.0 1.0
HB3 A:ASP192 4.9 28.6 1.0
HB2 A:TYR193 4.9 32.4 1.0
HB2 A:ASP71 4.9 21.2 1.0
HD1 A:TYR193 5.0 25.8 1.0
CG2 A:ILE189 5.0 21.5 1.0

Calcium binding site 2 out of 2 in 6dmn

Go back to Calcium Binding Sites List in 6dmn
Calcium binding site 2 out of 2 in the Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with An Rna/Dna Hybrid: Soaked in 2 Mm CA2+ and 200 Mm K+ at 21 C within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca202

b:16.5
occ:0.90
OD1 A:ASP71 2.2 19.2 1.0
OD1 A:ASP132 2.4 16.2 1.0
OE1 A:GLU109 2.4 18.3 1.0
OD2 A:ASP132 2.5 19.0 1.0
OP1 b:C5 2.6 18.6 1.0
CG A:ASP132 2.8 16.7 1.0
O A:HOH355 2.8 16.3 1.0
O3' B:U4 2.8 17.9 1.0
OE2 A:GLU109 3.0 16.6 1.0
CD A:GLU109 3.1 17.0 1.0
CG A:ASP71 3.2 18.1 1.0
P b:C5 3.3 18.1 1.0
OD2 A:ASP71 3.6 22.1 1.0
HG12 A:VAL72 3.8 19.1 1.0
H A:VAL72 3.8 18.9 1.0
HA A:ASP71 3.9 17.5 1.0
C3' B:U4 4.1 16.7 1.0
H A:ASP132 4.1 17.1 1.0
C5' b:C5 4.2 17.4 1.0
CB A:ASP132 4.2 16.6 1.0
CA A:CA201 4.2 17.7 0.8
HG21 A:ILE189 4.3 25.8 1.0
O A:HOH340 4.3 23.5 0.8
O5' b:C5 4.3 17.4 1.0
O2' B:U4 4.3 16.7 1.0
CB A:ASP71 4.3 17.7 1.0
HB2 A:SER133 4.4 18.0 1.0
C4' B:U4 4.4 16.6 1.0
OP2 b:C5 4.5 21.2 1.0
HB3 A:ASP132 4.6 19.9 1.0
HB2 A:ASP71 4.6 21.2 1.0
CG A:GLU109 4.6 16.5 1.0
CA A:ASP71 4.6 14.6 1.0
N A:VAL72 4.6 15.8 1.0
HG22 A:ILE189 4.7 25.8 1.0
CG1 A:VAL72 4.7 15.9 1.0
HB2 A:ASP132 4.7 19.9 1.0
N A:ASP132 4.8 14.3 1.0
C2' B:U4 4.8 15.9 1.0
HG3 A:GLU109 4.9 19.9 1.0
CA A:ASP132 4.9 15.3 1.0
HG2 A:GLU109 4.9 19.9 1.0
CG2 A:ILE189 5.0 21.5 1.0

Reference:

N.L.Samara, W.Yang. Cation Trafficking Propels Rna Hydrolysis. Nat. Struct. Mol. Biol. V. 25 715 2018.
ISSN: ESSN 1545-9985
PubMed: 30076410
DOI: 10.1038/S41594-018-0099-4
Page generated: Mon Jul 15 17:58:58 2024

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