Calcium in PDB 6erc: Peroxidase A From Dictyostelium Discoideum (Ddpoxa)
Enzymatic activity of Peroxidase A From Dictyostelium Discoideum (Ddpoxa)
All present enzymatic activity of Peroxidase A From Dictyostelium Discoideum (Ddpoxa):
1.11.1.7;
Protein crystallography data
The structure of Peroxidase A From Dictyostelium Discoideum (Ddpoxa), PDB code: 6erc
was solved by
A.Nicolussi,
G.Mlynek,
P.G.Furtmueller,
K.Djinovic-Carugo,
C.Obinger,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
48.67 /
2.50
|
Space group
|
P 31 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
128.253,
128.253,
146.015,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
22.8 /
25.7
|
Other elements in 6erc:
The structure of Peroxidase A From Dictyostelium Discoideum (Ddpoxa) also contains other interesting chemical elements:
Calcium Binding Sites:
The binding sites of Calcium atom in the Peroxidase A From Dictyostelium Discoideum (Ddpoxa)
(pdb code 6erc). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the
Peroxidase A From Dictyostelium Discoideum (Ddpoxa), PDB code: 6erc:
Jump to Calcium binding site number:
1;
2;
Calcium binding site 1 out
of 2 in 6erc
Go back to
Calcium Binding Sites List in 6erc
Calcium binding site 1 out
of 2 in the Peroxidase A From Dictyostelium Discoideum (Ddpoxa)
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Peroxidase A From Dictyostelium Discoideum (Ddpoxa) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca605
b:47.4
occ:1.00
|
HG
|
A:SER326
|
2.1
|
65.7
|
1.0
|
OG
|
A:SER326
|
2.3
|
54.7
|
1.0
|
O
|
A:HOH755
|
2.3
|
42.9
|
1.0
|
OD1
|
A:ASP377
|
2.4
|
54.5
|
1.0
|
O
|
A:TYR379
|
2.4
|
56.9
|
1.0
|
O
|
A:HOH707
|
2.4
|
42.5
|
1.0
|
O
|
A:ASN375
|
2.4
|
54.6
|
1.0
|
O
|
A:SER326
|
2.5
|
54.6
|
1.0
|
C
|
A:SER326
|
3.2
|
54.6
|
1.0
|
HA
|
A:SER326
|
3.3
|
64.4
|
1.0
|
HG3
|
A:GLU374
|
3.3
|
69.2
|
1.0
|
H
|
A:ASP377
|
3.3
|
69.7
|
1.0
|
HB2
|
A:TYR379
|
3.4
|
69.3
|
1.0
|
CB
|
A:SER326
|
3.4
|
53.5
|
1.0
|
CG
|
A:ASP377
|
3.4
|
56.4
|
1.0
|
C
|
A:TYR379
|
3.5
|
56.5
|
1.0
|
CA
|
A:SER326
|
3.5
|
53.6
|
1.0
|
C
|
A:ASN375
|
3.6
|
55.1
|
1.0
|
H
|
A:TYR379
|
3.8
|
69.2
|
1.0
|
HG2
|
A:GLU374
|
3.8
|
69.2
|
1.0
|
OD2
|
A:ASP377
|
3.9
|
57.5
|
1.0
|
HB3
|
A:SER326
|
3.9
|
64.2
|
1.0
|
HA
|
A:ILE376
|
3.9
|
68.8
|
1.0
|
N
|
A:ASP377
|
4.0
|
58.1
|
1.0
|
CG
|
A:GLU374
|
4.0
|
57.7
|
1.0
|
HB2
|
A:SER326
|
4.1
|
64.2
|
1.0
|
HA
|
A:MET380
|
4.1
|
67.7
|
1.0
|
CA
|
A:TYR379
|
4.1
|
58.1
|
1.0
|
HB3
|
A:GLU374
|
4.1
|
65.6
|
1.0
|
OE1
|
A:GLN78
|
4.1
|
57.8
|
1.0
|
CB
|
A:TYR379
|
4.2
|
57.7
|
1.0
|
O
|
A:ASP377
|
4.2
|
57.7
|
1.0
|
N
|
A:TYR379
|
4.2
|
57.7
|
1.0
|
O
|
A:HOH752
|
4.3
|
43.4
|
1.0
|
HA
|
A:GLU327
|
4.3
|
66.0
|
1.0
|
H
|
A:ASN375
|
4.3
|
69.3
|
1.0
|
HE22
|
A:GLN78
|
4.3
|
68.8
|
1.0
|
HB3
|
A:ASN375
|
4.3
|
68.6
|
1.0
|
N
|
A:GLU327
|
4.4
|
53.0
|
1.0
|
N
|
A:ASN375
|
4.4
|
57.7
|
1.0
|
N
|
A:MET380
|
4.5
|
55.8
|
1.0
|
N
|
A:ILE376
|
4.5
|
56.3
|
1.0
|
C
|
A:ASP377
|
4.5
|
57.9
|
1.0
|
CA
|
A:ASN375
|
4.5
|
56.9
|
1.0
|
CA
|
A:ILE376
|
4.6
|
57.3
|
1.0
|
HD2
|
A:TYR379
|
4.6
|
69.4
|
1.0
|
CA
|
A:ASP377
|
4.6
|
57.9
|
1.0
|
CB
|
A:ASP377
|
4.6
|
58.1
|
1.0
|
CB
|
A:GLU374
|
4.6
|
54.7
|
1.0
|
HB3
|
A:TYR379
|
4.7
|
69.3
|
1.0
|
C
|
A:ILE376
|
4.7
|
59.0
|
1.0
|
CA
|
A:MET380
|
4.8
|
56.4
|
1.0
|
CD
|
A:GLN78
|
4.8
|
58.0
|
1.0
|
NE2
|
A:GLN78
|
4.8
|
57.3
|
1.0
|
HD13
|
A:ILE82
|
4.8
|
73.9
|
1.0
|
CA
|
A:GLU327
|
4.9
|
55.0
|
1.0
|
N
|
A:SER326
|
5.0
|
52.5
|
1.0
|
C
|
A:GLU374
|
5.0
|
55.5
|
1.0
|
CB
|
A:ASN375
|
5.0
|
57.2
|
1.0
|
|
Calcium binding site 2 out
of 2 in 6erc
Go back to
Calcium Binding Sites List in 6erc
Calcium binding site 2 out
of 2 in the Peroxidase A From Dictyostelium Discoideum (Ddpoxa)
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Peroxidase A From Dictyostelium Discoideum (Ddpoxa) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca601
b:51.8
occ:1.00
|
HG
|
B:SER326
|
2.1
|
70.0
|
1.0
|
OD1
|
B:ASP377
|
2.3
|
59.2
|
1.0
|
O
|
B:TYR379
|
2.3
|
59.2
|
1.0
|
OG
|
B:SER326
|
2.4
|
58.3
|
1.0
|
O
|
B:SER326
|
2.4
|
57.2
|
1.0
|
O
|
B:ASN375
|
2.4
|
60.5
|
1.0
|
O
|
B:HOH724
|
2.4
|
46.0
|
1.0
|
O
|
B:HOH748
|
2.5
|
48.2
|
1.0
|
H
|
B:ASP377
|
3.1
|
72.7
|
1.0
|
C
|
B:SER326
|
3.3
|
57.7
|
1.0
|
C
|
B:TYR379
|
3.5
|
60.5
|
1.0
|
CG
|
B:ASP377
|
3.5
|
61.0
|
1.0
|
HB2
|
B:TYR379
|
3.5
|
75.3
|
1.0
|
HA
|
B:SER326
|
3.5
|
67.8
|
1.0
|
CB
|
B:SER326
|
3.5
|
56.5
|
1.0
|
HB3
|
B:GLU374
|
3.5
|
74.7
|
1.0
|
C
|
B:ASN375
|
3.7
|
60.6
|
1.0
|
CA
|
B:SER326
|
3.7
|
56.5
|
1.0
|
H
|
B:TYR379
|
3.8
|
74.7
|
1.0
|
N
|
B:ASP377
|
3.9
|
60.5
|
1.0
|
HA
|
B:ILE376
|
4.0
|
69.6
|
1.0
|
HB3
|
B:SER326
|
4.1
|
67.7
|
1.0
|
OD2
|
B:ASP377
|
4.1
|
61.7
|
1.0
|
HA
|
B:MET380
|
4.1
|
70.0
|
1.0
|
HB2
|
B:SER326
|
4.2
|
67.7
|
1.0
|
O
|
B:ASP377
|
4.2
|
59.5
|
1.0
|
CA
|
B:TYR379
|
4.2
|
62.6
|
1.0
|
N
|
B:TYR379
|
4.2
|
62.3
|
1.0
|
HA
|
B:GLU327
|
4.2
|
71.9
|
1.0
|
CB
|
B:TYR379
|
4.3
|
62.7
|
1.0
|
H
|
B:ASN375
|
4.3
|
74.6
|
1.0
|
O
|
B:HOH750
|
4.4
|
43.0
|
1.0
|
N
|
B:ASN375
|
4.4
|
62.2
|
1.0
|
N
|
B:GLU327
|
4.4
|
58.1
|
1.0
|
CB
|
B:GLU374
|
4.4
|
62.3
|
1.0
|
C
|
B:ASP377
|
4.4
|
61.0
|
1.0
|
HE22
|
B:GLN78
|
4.5
|
76.2
|
1.0
|
OE1
|
B:GLN78
|
4.5
|
60.9
|
1.0
|
HB2
|
B:GLU374
|
4.5
|
74.7
|
1.0
|
N
|
B:MET380
|
4.5
|
59.7
|
1.0
|
CA
|
B:ASP377
|
4.5
|
60.4
|
1.0
|
HB3
|
B:ASN375
|
4.6
|
73.8
|
1.0
|
N
|
B:ILE376
|
4.6
|
59.6
|
1.0
|
CA
|
B:ASN375
|
4.6
|
61.8
|
1.0
|
CA
|
B:ILE376
|
4.6
|
58.0
|
1.0
|
CB
|
B:ASP377
|
4.6
|
61.6
|
1.0
|
C
|
B:ILE376
|
4.7
|
59.4
|
1.0
|
HB3
|
B:TYR379
|
4.8
|
75.3
|
1.0
|
HD2
|
B:TYR379
|
4.8
|
78.2
|
1.0
|
CA
|
B:MET380
|
4.8
|
58.3
|
1.0
|
CA
|
B:GLU327
|
4.8
|
59.9
|
1.0
|
C
|
B:GLU374
|
4.9
|
61.2
|
1.0
|
NE2
|
B:GLN78
|
4.9
|
63.5
|
1.0
|
CD
|
B:GLN78
|
5.0
|
63.0
|
1.0
|
|
Reference:
A.Nicolussi,
J.D.Dunn,
G.Mlynek,
M.Bellei,
M.Zamocky,
G.Battistuzzi,
K.Djinovic-Carugo,
P.G.Furtmuller,
T.Soldati,
C.Obinger.
Secreted Heme Peroxidase From Dictyostelium Discoideum: Insights Into Catalysis, Structure, and Biological Role. J. Biol. Chem. V. 293 1330 2018.
ISSN: ESSN 1083-351X
PubMed: 29242189
DOI: 10.1074/JBC.RA117.000463
Page generated: Mon Jul 15 18:37:14 2024
|